Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltK and gltJ overlap by 1 nucleotides gltJ and gltI are separated by 169 nucleotides gltI and insH-3 are separated by 249 nucleotides
b0653: gltK - glutamate and aspartate transporter subunit (NCBI), at 684,478 to 685,152
gltK
b0654: gltJ - glutamate and aspartate transporter subunit (NCBI), at 685,152 to 685,892
gltJ
b0655: gltI - glutamate and aspartate transporter subunit (NCBI), at 686,062 to 686,970
gltI
b0656: insH-3 - IS5 transposase and trans-activator (NCBI), at 687,220 to 688,236
insH-3
Position (kb) 686
687 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 685.128 kb on - strand at 685.273 kb on - strand, within gltJ at 685.314 kb on + strand, within gltJ at 685.314 kb on + strand, within gltJ at 685.367 kb on + strand, within gltJ at 685.421 kb on + strand, within gltJ at 685.567 kb on + strand, within gltJ at 685.567 kb on + strand, within gltJ at 685.588 kb on + strand, within gltJ at 685.603 kb on + strand, within gltJ at 685.603 kb on + strand, within gltJ at 685.687 kb on + strand, within gltJ at 685.687 kb on + strand, within gltJ at 685.695 kb on - strand, within gltJ at 685.695 kb on - strand, within gltJ at 685.701 kb on - strand, within gltJ at 685.737 kb on - strand, within gltJ at 685.737 kb on - strand, within gltJ at 685.741 kb on - strand, within gltJ at 685.831 kb on + strand at 685.831 kb on - strand at 685.831 kb on - strand at 685.883 kb on - strand at 685.945 kb on + strand at 685.958 kb on - strand at 685.965 kb on - strand at 685.965 kb on - strand at 685.995 kb on + strand at 685.995 kb on + strand at 686.001 kb on + strand at 686.001 kb on + strand at 686.022 kb on - strand at 686.029 kb on - strand at 686.029 kb on - strand at 686.060 kb on + strand at 686.060 kb on + strand at 686.089 kb on + strand at 686.105 kb on - strand at 686.197 kb on + strand, within gltI at 686.276 kb on + strand, within gltI at 686.341 kb on + strand, within gltI at 686.770 kb on - strand, within gltI at 686.876 kb on + strand, within gltI at 686.884 kb on - strand at 687.053 kb on + strand at 687.053 kb on + strand at 687.066 kb on - strand at 687.066 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 685,128 - -2.4 685,273 - gltJ b0654 0.16 -0.6 685,314 + gltJ b0654 0.22 +0.2 685,314 + gltJ b0654 0.22 -1.1 685,367 + gltJ b0654 0.29 -1.6 685,421 + gltJ b0654 0.36 -0.1 685,567 + gltJ b0654 0.56 -0.1 685,567 + gltJ b0654 0.56 +0.2 685,588 + gltJ b0654 0.59 -0.2 685,603 + gltJ b0654 0.61 +0.3 685,603 + gltJ b0654 0.61 -1.7 685,687 + gltJ b0654 0.72 +0.7 685,687 + gltJ b0654 0.72 -1.2 685,695 - gltJ b0654 0.73 -3.4 685,695 - gltJ b0654 0.73 -0.4 685,701 - gltJ b0654 0.74 -0.7 685,737 - gltJ b0654 0.79 -1.5 685,737 - gltJ b0654 0.79 -0.7 685,741 - gltJ b0654 0.79 -0.6 685,831 + +0.2 685,831 - -0.2 685,831 - -1.5 685,883 - -0.5 685,945 + +0.2 685,958 - -1.7 685,965 - -1.0 685,965 - +0.9 685,995 + +1.6 685,995 + -0.1 686,001 + +0.6 686,001 + -0.7 686,022 - -0.7 686,029 - -1.2 686,029 - +0.9 686,060 + +0.9 686,060 + +0.6 686,089 + -0.1 686,105 - -1.5 686,197 + gltI b0655 0.15 -0.6 686,276 + gltI b0655 0.24 +0.2 686,341 + gltI b0655 0.31 +2.1 686,770 - gltI b0655 0.78 +1.1 686,876 + gltI b0655 0.90 -0.2 686,884 - -0.2 687,053 + +0.6 687,053 + -0.6 687,066 - -2.3 687,066 - -0.0
Or see this region's nucleotide sequence