Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mrdA and ybeA are separated by 30 nucleotides ybeA and ybeB are separated by 3 nucleotides ybeB and cobC are separated by 259 nucleotides
b0635: mrdA - transpeptidase involved in peptidoglycan synthesis (penicillin-binding protein 2) (NCBI), at 665,539 to 667,440
mrdA
b0636: ybeA - hypothetical protein (NCBI), at 667,471 to 667,938
ybeA
b0637: ybeB - orf, hypothetical protein (VIMSS), at 667,942 to 668,259
ybeB
b0638: cobC - predicted alpha-ribazole-5'-P phosphatase (NCBI), at 668,519 to 669,130
cobC
Position (kb) 667
668 Strain fitness (log2 ratio) -2
-1
0
1
2 at 667.444 kb on - strand at 667.495 kb on - strand at 667.495 kb on - strand at 667.495 kb on - strand at 667.524 kb on - strand, within ybeA at 667.524 kb on - strand, within ybeA at 667.588 kb on - strand, within ybeA at 667.590 kb on - strand, within ybeA at 667.665 kb on - strand, within ybeA at 667.665 kb on - strand, within ybeA at 667.703 kb on - strand, within ybeA at 667.703 kb on - strand, within ybeA at 667.762 kb on - strand, within ybeA at 667.762 kb on - strand, within ybeA at 667.852 kb on - strand, within ybeA at 667.852 kb on - strand, within ybeA at 668.424 kb on + strand at 668.466 kb on - strand at 668.466 kb on - strand at 668.601 kb on - strand, within cobC at 668.672 kb on + strand, within cobC at 668.680 kb on - strand, within cobC at 668.733 kb on + strand, within cobC at 668.733 kb on + strand, within cobC at 668.796 kb on - strand, within cobC at 668.796 kb on - strand, within cobC at 668.930 kb on + strand, within cobC
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 667,444 - +0.9 667,495 - +1.3 667,495 - +0.9 667,495 - +1.3 667,524 - ybeA b0636 0.11 +1.6 667,524 - ybeA b0636 0.11 +1.6 667,588 - ybeA b0636 0.25 +1.6 667,590 - ybeA b0636 0.25 +1.4 667,665 - ybeA b0636 0.41 +1.1 667,665 - ybeA b0636 0.41 -0.3 667,703 - ybeA b0636 0.50 +1.5 667,703 - ybeA b0636 0.50 +0.7 667,762 - ybeA b0636 0.62 +2.1 667,762 - ybeA b0636 0.62 +0.9 667,852 - ybeA b0636 0.81 +1.9 667,852 - ybeA b0636 0.81 +2.4 668,424 + -1.3 668,466 - -0.5 668,466 - -0.7 668,601 - cobC b0638 0.13 +1.3 668,672 + cobC b0638 0.25 -0.5 668,680 - cobC b0638 0.26 +0.1 668,733 + cobC b0638 0.35 -1.0 668,733 + cobC b0638 0.35 -1.0 668,796 - cobC b0638 0.45 -2.0 668,796 - cobC b0638 0.45 -1.1 668,930 + cobC b0638 0.67 -0.4
Or see this region's nucleotide sequence