Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbY and glxK are separated by 21 nucleotides glxK and ylbA are separated by 227 nucleotides
b0513: ybbY - putative transport (VIMSS), at 539,789 to 541,090
ybbY
b0514: glxK - glycerate kinase II (NCBI), at 541,112 to 542,257
glxK
b0515: ylbA - hypothetical protein (NCBI), at 542,485 to 543,270
ylbA
Position (kb) 541
542
543 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 540.149 kb on + strand, within ybbY at 540.315 kb on + strand, within ybbY at 540.325 kb on + strand, within ybbY at 540.325 kb on + strand, within ybbY at 540.404 kb on - strand, within ybbY at 540.404 kb on - strand, within ybbY at 540.446 kb on + strand, within ybbY at 540.446 kb on + strand, within ybbY at 540.455 kb on - strand, within ybbY at 540.455 kb on - strand, within ybbY at 540.484 kb on - strand, within ybbY at 540.502 kb on + strand, within ybbY at 540.526 kb on + strand, within ybbY at 540.531 kb on - strand, within ybbY at 540.554 kb on + strand, within ybbY at 540.554 kb on + strand, within ybbY at 540.593 kb on + strand, within ybbY at 540.593 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.612 kb on + strand, within ybbY at 540.613 kb on + strand, within ybbY at 540.622 kb on + strand, within ybbY at 540.622 kb on + strand, within ybbY at 540.784 kb on + strand, within ybbY at 540.787 kb on - strand, within ybbY at 540.792 kb on - strand, within ybbY at 540.803 kb on + strand, within ybbY at 540.804 kb on - strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.813 kb on - strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.824 kb on + strand, within ybbY at 540.825 kb on - strand, within ybbY at 540.829 kb on + strand, within ybbY at 540.831 kb on + strand, within ybbY at 540.834 kb on + strand, within ybbY at 540.854 kb on + strand, within ybbY at 540.854 kb on + strand, within ybbY at 540.860 kb on + strand, within ybbY at 540.880 kb on + strand, within ybbY at 540.880 kb on + strand, within ybbY at 540.882 kb on + strand, within ybbY at 540.917 kb on + strand, within ybbY at 540.967 kb on + strand at 540.971 kb on + strand at 540.976 kb on + strand at 540.980 kb on - strand at 540.987 kb on + strand at 540.987 kb on + strand at 541.001 kb on + strand at 541.001 kb on + strand at 541.001 kb on - strand at 541.005 kb on + strand at 541.007 kb on + strand at 541.007 kb on + strand at 541.010 kb on + strand at 541.010 kb on + strand at 541.010 kb on + strand at 541.010 kb on + strand at 541.012 kb on + strand at 541.012 kb on + strand at 541.018 kb on - strand at 541.018 kb on - strand at 541.020 kb on - strand at 541.020 kb on - strand at 541.023 kb on + strand at 541.023 kb on + strand at 541.029 kb on - strand at 541.035 kb on + strand at 541.062 kb on + strand at 541.062 kb on + strand at 541.070 kb on - strand at 541.070 kb on - strand at 541.070 kb on - strand at 541.070 kb on - strand at 541.125 kb on - strand at 541.135 kb on - strand at 541.155 kb on + strand at 541.177 kb on + strand at 541.177 kb on + strand at 541.266 kb on - strand, within glxK at 541.269 kb on - strand, within glxK at 541.295 kb on - strand, within glxK at 541.296 kb on + strand, within glxK at 541.432 kb on - strand, within glxK at 541.452 kb on - strand, within glxK at 541.452 kb on - strand, within glxK at 541.472 kb on + strand, within glxK at 541.502 kb on - strand, within glxK at 541.545 kb on + strand, within glxK at 541.547 kb on + strand, within glxK at 541.560 kb on + strand, within glxK at 541.560 kb on + strand, within glxK at 541.637 kb on - strand, within glxK at 541.644 kb on + strand, within glxK at 541.719 kb on + strand, within glxK at 541.740 kb on - strand, within glxK at 541.866 kb on + strand, within glxK at 541.875 kb on + strand, within glxK at 541.886 kb on + strand, within glxK at 541.960 kb on - strand, within glxK at 541.968 kb on - strand, within glxK at 542.031 kb on + strand, within glxK at 542.031 kb on + strand, within glxK at 542.031 kb on + strand, within glxK at 542.039 kb on - strand, within glxK at 542.043 kb on - strand, within glxK at 542.053 kb on + strand, within glxK at 542.053 kb on + strand, within glxK at 542.070 kb on + strand, within glxK at 542.073 kb on + strand, within glxK at 542.086 kb on + strand, within glxK at 542.097 kb on + strand, within glxK at 542.105 kb on + strand, within glxK at 542.113 kb on - strand, within glxK at 542.119 kb on - strand, within glxK at 542.121 kb on + strand, within glxK at 542.150 kb on + strand at 542.151 kb on + strand at 542.163 kb on + strand at 542.173 kb on + strand at 542.173 kb on + strand at 542.184 kb on + strand at 542.195 kb on + strand at 542.195 kb on + strand at 542.195 kb on + strand at 542.197 kb on + strand at 542.209 kb on + strand at 542.228 kb on + strand at 542.228 kb on + strand at 542.282 kb on + strand at 542.288 kb on + strand at 542.290 kb on + strand at 542.298 kb on + strand at 542.303 kb on - strand at 542.306 kb on + strand at 542.306 kb on + strand at 542.306 kb on + strand at 542.306 kb on - strand at 542.306 kb on - strand at 542.306 kb on - strand at 542.306 kb on - strand at 542.313 kb on - strand at 542.314 kb on - strand at 542.314 kb on - strand at 542.316 kb on - strand at 542.316 kb on - strand at 542.316 kb on - strand at 542.319 kb on + strand at 542.319 kb on + strand at 542.320 kb on + strand at 542.321 kb on + strand at 542.321 kb on + strand at 542.329 kb on + strand at 542.329 kb on + strand at 542.337 kb on - strand at 542.337 kb on - strand at 542.338 kb on - strand at 542.348 kb on - strand at 542.358 kb on + strand at 542.358 kb on + strand at 542.366 kb on + strand at 542.377 kb on + strand at 542.384 kb on - strand at 542.385 kb on - strand at 542.387 kb on + strand at 542.387 kb on + strand at 542.389 kb on + strand at 542.395 kb on + strand at 542.395 kb on + strand at 542.395 kb on - strand at 542.395 kb on - strand at 542.399 kb on + strand at 542.399 kb on + strand at 542.402 kb on + strand at 542.407 kb on - strand at 542.408 kb on - strand at 542.417 kb on - strand at 542.417 kb on - strand at 542.421 kb on + strand at 542.421 kb on + strand at 542.437 kb on - strand at 542.437 kb on - strand at 542.486 kb on - strand at 542.486 kb on - strand at 542.486 kb on - strand at 542.486 kb on - strand at 542.491 kb on - strand at 542.498 kb on - strand at 542.520 kb on + strand at 542.528 kb on - strand at 542.528 kb on - strand at 542.528 kb on - strand at 542.528 kb on - strand at 542.541 kb on - strand at 542.543 kb on - strand at 542.544 kb on + strand at 542.544 kb on + strand at 542.544 kb on + strand at 542.544 kb on + strand at 542.544 kb on + strand at 542.544 kb on + strand at 542.544 kb on + strand at 542.544 kb on + strand at 542.547 kb on + strand at 542.547 kb on + strand at 542.547 kb on + strand at 542.547 kb on + strand at 542.550 kb on + strand at 542.550 kb on + strand at 542.552 kb on - strand at 542.552 kb on - strand at 542.552 kb on - strand at 542.552 kb on - strand at 542.552 kb on - strand at 542.553 kb on - strand at 542.555 kb on + strand at 542.559 kb on - strand at 542.559 kb on - strand at 542.559 kb on - strand at 542.563 kb on - strand at 542.563 kb on - strand at 542.563 kb on - strand at 542.573 kb on - strand, within ylbA at 542.573 kb on - strand, within ylbA at 542.578 kb on - strand, within ylbA at 542.660 kb on - strand, within ylbA at 542.675 kb on - strand, within ylbA at 542.695 kb on - strand, within ylbA at 542.695 kb on - strand, within ylbA at 542.709 kb on + strand, within ylbA at 542.712 kb on - strand, within ylbA at 542.724 kb on - strand, within ylbA at 542.733 kb on - strand, within ylbA at 542.733 kb on - strand, within ylbA at 542.734 kb on - strand, within ylbA at 542.741 kb on - strand, within ylbA at 542.751 kb on - strand, within ylbA at 542.758 kb on - strand, within ylbA at 542.758 kb on - strand, within ylbA at 542.780 kb on - strand, within ylbA at 542.780 kb on - strand, within ylbA at 542.782 kb on + strand, within ylbA at 542.801 kb on - strand, within ylbA at 542.801 kb on - strand, within ylbA at 542.803 kb on + strand, within ylbA at 542.813 kb on - strand, within ylbA at 542.813 kb on - strand, within ylbA at 542.841 kb on + strand, within ylbA at 542.841 kb on + strand, within ylbA at 542.846 kb on - strand, within ylbA at 542.849 kb on - strand, within ylbA at 542.849 kb on - strand, within ylbA at 542.849 kb on - strand, within ylbA at 542.856 kb on + strand, within ylbA at 542.856 kb on - strand, within ylbA at 542.856 kb on - strand, within ylbA at 542.859 kb on + strand, within ylbA at 542.869 kb on - strand, within ylbA at 542.869 kb on - strand, within ylbA at 542.893 kb on + strand, within ylbA at 542.893 kb on + strand, within ylbA at 542.894 kb on + strand, within ylbA at 542.901 kb on - strand, within ylbA at 542.905 kb on + strand, within ylbA at 542.905 kb on + strand, within ylbA at 542.908 kb on - strand, within ylbA at 542.913 kb on - strand, within ylbA at 542.927 kb on - strand, within ylbA at 542.929 kb on - strand, within ylbA at 542.936 kb on + strand, within ylbA at 542.936 kb on + strand, within ylbA at 542.937 kb on - strand, within ylbA at 542.993 kb on + strand, within ylbA at 543.001 kb on - strand, within ylbA at 543.001 kb on - strand, within ylbA at 543.046 kb on - strand, within ylbA at 543.056 kb on - strand, within ylbA at 543.064 kb on - strand, within ylbA at 543.067 kb on - strand, within ylbA at 543.082 kb on - strand, within ylbA at 543.082 kb on - strand, within ylbA at 543.084 kb on + strand, within ylbA at 543.116 kb on - strand, within ylbA at 543.116 kb on - strand, within ylbA at 543.118 kb on + strand, within ylbA at 543.121 kb on - strand, within ylbA at 543.123 kb on + strand, within ylbA at 543.179 kb on - strand, within ylbA at 543.187 kb on - strand, within ylbA at 543.189 kb on - strand, within ylbA at 543.189 kb on - strand, within ylbA at 543.189 kb on - strand, within ylbA at 543.189 kb on - strand, within ylbA at 543.194 kb on + strand at 543.212 kb on - strand at 543.212 kb on - strand at 543.226 kb on + strand at 543.234 kb on - strand at 543.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 540,149 + ybbY b0513 0.28 -2.3 540,315 + ybbY b0513 0.40 +1.6 540,325 + ybbY b0513 0.41 -2.1 540,325 + ybbY b0513 0.41 -0.4 540,404 - ybbY b0513 0.47 -0.1 540,404 - ybbY b0513 0.47 +0.7 540,446 + ybbY b0513 0.50 -0.2 540,446 + ybbY b0513 0.50 -1.8 540,455 - ybbY b0513 0.51 +0.2 540,455 - ybbY b0513 0.51 -1.4 540,484 - ybbY b0513 0.53 +0.1 540,502 + ybbY b0513 0.55 +0.1 540,526 + ybbY b0513 0.57 -0.2 540,531 - ybbY b0513 0.57 +0.8 540,554 + ybbY b0513 0.59 +0.1 540,554 + ybbY b0513 0.59 -1.3 540,593 + ybbY b0513 0.62 -0.4 540,593 + ybbY b0513 0.62 -1.2 540,607 + ybbY b0513 0.63 -0.7 540,607 + ybbY b0513 0.63 -0.6 540,607 + ybbY b0513 0.63 -0.9 540,607 + ybbY b0513 0.63 -0.6 540,612 + ybbY b0513 0.63 -0.2 540,613 + ybbY b0513 0.63 -0.6 540,622 + ybbY b0513 0.64 -1.3 540,622 + ybbY b0513 0.64 -1.0 540,784 + ybbY b0513 0.76 -0.8 540,787 - ybbY b0513 0.77 -1.6 540,792 - ybbY b0513 0.77 +3.6 540,803 + ybbY b0513 0.78 -1.9 540,804 - ybbY b0513 0.78 +0.4 540,805 + ybbY b0513 0.78 -2.3 540,805 + ybbY b0513 0.78 -1.1 540,805 + ybbY b0513 0.78 -3.4 540,805 + ybbY b0513 0.78 -1.0 540,805 + ybbY b0513 0.78 +0.0 540,813 - ybbY b0513 0.79 +3.6 540,817 + ybbY b0513 0.79 +2.8 540,817 + ybbY b0513 0.79 +0.6 540,817 + ybbY b0513 0.79 -1.1 540,817 + ybbY b0513 0.79 -0.3 540,817 + ybbY b0513 0.79 -0.7 540,817 + ybbY b0513 0.79 -0.0 540,824 + ybbY b0513 0.79 +1.0 540,825 - ybbY b0513 0.80 +1.3 540,829 + ybbY b0513 0.80 -0.8 540,831 + ybbY b0513 0.80 -0.9 540,834 + ybbY b0513 0.80 -2.1 540,854 + ybbY b0513 0.82 +0.3 540,854 + ybbY b0513 0.82 -0.4 540,860 + ybbY b0513 0.82 +0.1 540,880 + ybbY b0513 0.84 -1.0 540,880 + ybbY b0513 0.84 -0.9 540,882 + ybbY b0513 0.84 -0.1 540,917 + ybbY b0513 0.87 +0.4 540,967 + +0.6 540,971 + -1.0 540,976 + -0.7 540,980 - -0.2 540,987 + -0.1 540,987 + +0.0 541,001 + +0.9 541,001 + -0.2 541,001 - -0.4 541,005 + +0.9 541,007 + +0.4 541,007 + +0.2 541,010 + +0.0 541,010 + -0.3 541,010 + -1.5 541,010 + -2.2 541,012 + -0.5 541,012 + -1.0 541,018 - -0.8 541,018 - -2.2 541,020 - -1.2 541,020 - +0.0 541,023 + -0.9 541,023 + -2.0 541,029 - +3.6 541,035 + -0.6 541,062 + -0.3 541,062 + -1.0 541,070 - -1.0 541,070 - -0.3 541,070 - -0.2 541,070 - +3.6 541,125 - -1.3 541,135 - -1.5 541,155 + -0.9 541,177 + -0.2 541,177 + +3.6 541,266 - glxK b0514 0.13 -0.2 541,269 - glxK b0514 0.14 -0.5 541,295 - glxK b0514 0.16 +0.4 541,296 + glxK b0514 0.16 -1.0 541,432 - glxK b0514 0.28 -3.6 541,452 - glxK b0514 0.30 -0.6 541,452 - glxK b0514 0.30 -0.7 541,472 + glxK b0514 0.31 +0.7 541,502 - glxK b0514 0.34 -0.1 541,545 + glxK b0514 0.38 -0.8 541,547 + glxK b0514 0.38 -1.6 541,560 + glxK b0514 0.39 -0.4 541,560 + glxK b0514 0.39 -1.8 541,637 - glxK b0514 0.46 -0.3 541,644 + glxK b0514 0.46 -1.0 541,719 + glxK b0514 0.53 -0.3 541,740 - glxK b0514 0.55 +0.5 541,866 + glxK b0514 0.66 -0.5 541,875 + glxK b0514 0.67 -1.1 541,886 + glxK b0514 0.68 -0.3 541,960 - glxK b0514 0.74 -0.3 541,968 - glxK b0514 0.75 +0.2 542,031 + glxK b0514 0.80 +1.2 542,031 + glxK b0514 0.80 +4.8 542,031 + glxK b0514 0.80 +4.9 542,039 - glxK b0514 0.81 +2.3 542,043 - glxK b0514 0.81 -2.5 542,053 + glxK b0514 0.82 +0.5 542,053 + glxK b0514 0.82 +0.2 542,070 + glxK b0514 0.84 -1.8 542,073 + glxK b0514 0.84 +0.6 542,086 + glxK b0514 0.85 +0.1 542,097 + glxK b0514 0.86 -0.6 542,105 + glxK b0514 0.87 +0.2 542,113 - glxK b0514 0.87 +3.6 542,119 - glxK b0514 0.88 +3.6 542,121 + glxK b0514 0.88 -0.2 542,150 + +2.0 542,151 + -0.5 542,163 + +0.1 542,173 + -0.1 542,173 + +1.1 542,184 + -2.2 542,195 + -0.5 542,195 + -1.3 542,195 + +0.6 542,197 + +0.2 542,209 + -1.7 542,228 + -1.0 542,228 + +0.1 542,282 + +0.0 542,288 + -0.9 542,290 + +0.7 542,298 + -1.6 542,303 - +1.3 542,306 + -0.3 542,306 + +0.1 542,306 + -0.4 542,306 - +0.8 542,306 - +2.6 542,306 - +3.6 542,306 - +3.6 542,313 - -0.2 542,314 - +1.6 542,314 - +3.6 542,316 - +3.6 542,316 - +1.0 542,316 - +2.0 542,319 + -0.3 542,319 + -0.1 542,320 + -0.2 542,321 + +0.4 542,321 + -1.4 542,329 + -1.0 542,329 + -0.9 542,337 - -0.6 542,337 - +0.3 542,338 - -0.6 542,348 - -1.4 542,358 + +0.3 542,358 + -0.7 542,366 + -0.5 542,377 + -0.3 542,384 - -0.7 542,385 - -0.6 542,387 + +0.0 542,387 + -0.1 542,389 + -1.2 542,395 + -1.4 542,395 + +0.2 542,395 - +0.1 542,395 - -0.5 542,399 + -0.3 542,399 + -0.9 542,402 + -1.2 542,407 - -1.8 542,408 - -1.9 542,417 - -0.6 542,417 - -0.1 542,421 + -1.3 542,421 + -0.5 542,437 - +0.6 542,437 - -0.3 542,486 - +0.9 542,486 - -2.5 542,486 - +1.2 542,486 - +0.5 542,491 - -0.2 542,498 - -0.5 542,520 + -0.2 542,528 - +1.6 542,528 - -2.0 542,528 - -0.5 542,528 - -0.6 542,541 - -1.0 542,543 - +0.4 542,544 + -0.2 542,544 + +3.6 542,544 + -0.3 542,544 + +0.5 542,544 + +1.3 542,544 + +1.4 542,544 + +2.6 542,544 + +1.6 542,547 + -0.7 542,547 + +0.8 542,547 + +0.8 542,547 + +0.8 542,550 + +2.6 542,550 + -0.2 542,552 - -0.4 542,552 - +0.1 542,552 - +0.0 542,552 - -2.0 542,552 - -0.4 542,553 - +0.5 542,555 + -0.5 542,559 - -0.9 542,559 - +1.6 542,559 - -0.4 542,563 - -1.6 542,563 - -0.3 542,563 - -0.8 542,573 - ylbA b0515 0.11 -1.4 542,573 - ylbA b0515 0.11 -1.5 542,578 - ylbA b0515 0.12 +0.6 542,660 - ylbA b0515 0.22 -0.3 542,675 - ylbA b0515 0.24 +0.7 542,695 - ylbA b0515 0.27 -1.5 542,695 - ylbA b0515 0.27 +1.4 542,709 + ylbA b0515 0.28 +3.6 542,712 - ylbA b0515 0.29 -1.1 542,724 - ylbA b0515 0.30 +0.0 542,733 - ylbA b0515 0.32 -0.3 542,733 - ylbA b0515 0.32 -1.8 542,734 - ylbA b0515 0.32 +0.0 542,741 - ylbA b0515 0.33 -0.6 542,751 - ylbA b0515 0.34 -1.3 542,758 - ylbA b0515 0.35 +0.1 542,758 - ylbA b0515 0.35 -0.9 542,780 - ylbA b0515 0.38 -0.7 542,780 - ylbA b0515 0.38 -0.2 542,782 + ylbA b0515 0.38 +2.0 542,801 - ylbA b0515 0.40 +0.8 542,801 - ylbA b0515 0.40 +0.6 542,803 + ylbA b0515 0.40 +3.6 542,813 - ylbA b0515 0.42 -0.8 542,813 - ylbA b0515 0.42 -1.9 542,841 + ylbA b0515 0.45 +0.4 542,841 + ylbA b0515 0.45 -1.0 542,846 - ylbA b0515 0.46 -1.4 542,849 - ylbA b0515 0.46 +0.3 542,849 - ylbA b0515 0.46 -1.1 542,849 - ylbA b0515 0.46 -1.3 542,856 + ylbA b0515 0.47 -2.7 542,856 - ylbA b0515 0.47 -0.6 542,856 - ylbA b0515 0.47 +0.8 542,859 + ylbA b0515 0.48 -0.2 542,869 - ylbA b0515 0.49 +0.9 542,869 - ylbA b0515 0.49 +1.2 542,893 + ylbA b0515 0.52 -0.7 542,893 + ylbA b0515 0.52 -0.4 542,894 + ylbA b0515 0.52 -0.6 542,901 - ylbA b0515 0.53 +0.0 542,905 + ylbA b0515 0.53 +1.0 542,905 + ylbA b0515 0.53 +0.2 542,908 - ylbA b0515 0.54 -0.7 542,913 - ylbA b0515 0.54 -0.3 542,927 - ylbA b0515 0.56 -0.8 542,929 - ylbA b0515 0.56 -1.2 542,936 + ylbA b0515 0.57 +0.5 542,936 + ylbA b0515 0.57 -0.5 542,937 - ylbA b0515 0.58 +0.3 542,993 + ylbA b0515 0.65 +1.6 543,001 - ylbA b0515 0.66 +0.6 543,001 - ylbA b0515 0.66 +0.1 543,046 - ylbA b0515 0.71 -0.3 543,056 - ylbA b0515 0.73 -0.5 543,064 - ylbA b0515 0.74 -0.2 543,067 - ylbA b0515 0.74 -0.7 543,082 - ylbA b0515 0.76 +1.4 543,082 - ylbA b0515 0.76 -0.2 543,084 + ylbA b0515 0.76 -0.5 543,116 - ylbA b0515 0.80 +0.4 543,116 - ylbA b0515 0.80 +0.8 543,118 + ylbA b0515 0.81 -0.5 543,121 - ylbA b0515 0.81 -1.2 543,123 + ylbA b0515 0.81 +0.2 543,179 - ylbA b0515 0.88 +0.3 543,187 - ylbA b0515 0.89 -1.6 543,189 - ylbA b0515 0.90 -0.9 543,189 - ylbA b0515 0.90 -1.1 543,189 - ylbA b0515 0.90 +0.1 543,189 - ylbA b0515 0.90 +0.0 543,194 + -0.2 543,212 - -0.9 543,212 - -0.4 543,226 + +3.6 543,234 - -0.4 543,234 - -1.4
Or see this region's nucleotide sequence