Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbK and ybbL are separated by 145 nucleotides ybbL and ybbM overlap by 14 nucleotides ybbM and ybbN are separated by 62 nucleotides ybbN and ybbO are separated by 60 nucleotides
b0489: ybbK - predicted protease, membrane anchored (NCBI), at 514,080 to 514,997
ybbK
b0490: ybbL - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 515,143 to 515,820
ybbL
b0491: ybbM - putative metal resistance protein (VIMSS), at 515,807 to 516,586
ybbM
b0492: ybbN - putative thioredoxin-like protein (VIMSS), at 516,649 to 517,503
ybbN
b0493: ybbO - short chain dehydrogenase (NCBI), at 517,564 to 518,373
ybbO
Position (kb) 515
516
517 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 514.851 kb on - strand, within ybbK at 514.853 kb on + strand, within ybbK at 514.853 kb on + strand, within ybbK at 514.863 kb on - strand, within ybbK at 514.863 kb on - strand, within ybbK at 514.907 kb on - strand at 514.907 kb on - strand at 514.959 kb on - strand at 515.010 kb on + strand at 515.018 kb on - strand at 515.018 kb on - strand at 515.018 kb on - strand at 515.020 kb on + strand at 515.020 kb on + strand at 515.020 kb on + strand at 515.021 kb on + strand at 515.021 kb on + strand at 515.055 kb on + strand at 515.184 kb on - strand at 515.187 kb on + strand at 515.313 kb on + strand, within ybbL at 515.313 kb on + strand, within ybbL at 515.332 kb on - strand, within ybbL at 515.344 kb on + strand, within ybbL at 515.360 kb on - strand, within ybbL at 515.360 kb on - strand, within ybbL at 515.373 kb on + strand, within ybbL at 515.385 kb on + strand, within ybbL at 515.391 kb on + strand, within ybbL at 515.391 kb on + strand, within ybbL at 515.399 kb on - strand, within ybbL at 515.399 kb on - strand, within ybbL at 515.400 kb on + strand, within ybbL at 515.400 kb on + strand, within ybbL at 515.402 kb on + strand, within ybbL at 515.408 kb on - strand, within ybbL at 515.410 kb on - strand, within ybbL at 515.413 kb on - strand, within ybbL at 515.414 kb on - strand, within ybbL at 515.431 kb on + strand, within ybbL at 515.434 kb on - strand, within ybbL at 515.439 kb on - strand, within ybbL at 515.450 kb on + strand, within ybbL at 515.473 kb on + strand, within ybbL at 515.474 kb on + strand, within ybbL at 515.474 kb on + strand, within ybbL at 515.549 kb on - strand, within ybbL at 515.553 kb on + strand, within ybbL at 515.661 kb on + strand, within ybbL at 515.661 kb on + strand, within ybbL at 515.710 kb on + strand, within ybbL at 515.715 kb on + strand, within ybbL at 515.718 kb on - strand, within ybbL at 515.718 kb on - strand, within ybbL at 515.754 kb on + strand at 515.768 kb on + strand at 515.768 kb on + strand at 515.770 kb on + strand at 515.783 kb on + strand at 515.783 kb on + strand at 515.803 kb on + strand at 515.803 kb on + strand at 515.845 kb on + strand at 515.846 kb on + strand at 515.847 kb on + strand at 515.847 kb on + strand at 515.847 kb on + strand at 515.847 kb on + strand at 515.852 kb on + strand at 515.852 kb on + strand at 515.853 kb on + strand at 515.853 kb on + strand at 515.872 kb on + strand at 515.907 kb on + strand, within ybbM at 515.947 kb on + strand, within ybbM at 515.982 kb on + strand, within ybbM at 515.987 kb on + strand, within ybbM at 515.991 kb on + strand, within ybbM at 515.991 kb on + strand, within ybbM at 516.009 kb on - strand, within ybbM at 516.010 kb on - strand, within ybbM at 516.010 kb on - strand, within ybbM at 516.118 kb on + strand, within ybbM at 516.118 kb on + strand, within ybbM at 516.120 kb on + strand, within ybbM at 516.122 kb on + strand, within ybbM at 516.122 kb on + strand, within ybbM at 516.127 kb on + strand, within ybbM at 516.141 kb on + strand, within ybbM at 516.144 kb on - strand, within ybbM at 516.144 kb on - strand, within ybbM at 516.149 kb on - strand, within ybbM at 516.149 kb on - strand, within ybbM at 516.149 kb on - strand, within ybbM at 516.150 kb on - strand, within ybbM at 516.150 kb on - strand, within ybbM at 516.202 kb on - strand, within ybbM at 516.213 kb on - strand, within ybbM at 516.216 kb on + strand, within ybbM at 516.216 kb on + strand, within ybbM at 516.217 kb on - strand, within ybbM at 516.224 kb on + strand, within ybbM at 516.245 kb on - strand, within ybbM at 516.265 kb on + strand, within ybbM at 516.310 kb on + strand, within ybbM at 516.310 kb on + strand, within ybbM at 516.313 kb on - strand, within ybbM at 516.313 kb on - strand, within ybbM at 516.331 kb on + strand, within ybbM at 516.393 kb on + strand, within ybbM at 516.393 kb on + strand, within ybbM at 516.393 kb on + strand, within ybbM at 516.404 kb on - strand, within ybbM at 516.414 kb on + strand, within ybbM at 516.414 kb on + strand, within ybbM at 516.473 kb on + strand, within ybbM at 516.479 kb on - strand, within ybbM at 516.489 kb on - strand, within ybbM at 516.634 kb on - strand at 516.636 kb on + strand at 516.636 kb on + strand at 516.644 kb on + strand at 516.667 kb on + strand at 516.667 kb on + strand at 516.670 kb on - strand at 516.707 kb on - strand at 516.824 kb on - strand, within ybbN at 516.922 kb on + strand, within ybbN at 516.972 kb on - strand, within ybbN at 516.972 kb on - strand, within ybbN at 516.986 kb on + strand, within ybbN at 517.018 kb on + strand, within ybbN at 517.026 kb on - strand, within ybbN at 517.026 kb on - strand, within ybbN at 517.096 kb on - strand, within ybbN at 517.096 kb on - strand, within ybbN at 517.181 kb on + strand, within ybbN at 517.255 kb on + strand, within ybbN at 517.308 kb on - strand, within ybbN at 517.308 kb on - strand, within ybbN at 517.340 kb on - strand, within ybbN at 517.345 kb on + strand, within ybbN at 517.401 kb on - strand, within ybbN at 517.433 kb on - strand at 517.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 514,851 - ybbK b0489 0.84 -0.7 514,853 + ybbK b0489 0.84 -0.9 514,853 + ybbK b0489 0.84 -0.2 514,863 - ybbK b0489 0.85 +0.5 514,863 - ybbK b0489 0.85 -1.6 514,907 - -0.9 514,907 - +0.3 514,959 - -0.1 515,010 + -1.0 515,018 - +0.4 515,018 - +2.8 515,018 - -1.2 515,020 + -1.0 515,020 + -0.2 515,020 + +0.9 515,021 + +0.4 515,021 + -1.3 515,055 + -1.0 515,184 - -0.9 515,187 + +0.4 515,313 + ybbL b0490 0.25 +4.0 515,313 + ybbL b0490 0.25 -1.1 515,332 - ybbL b0490 0.28 -0.3 515,344 + ybbL b0490 0.30 -0.9 515,360 - ybbL b0490 0.32 +0.3 515,360 - ybbL b0490 0.32 +0.3 515,373 + ybbL b0490 0.34 -0.9 515,385 + ybbL b0490 0.36 -0.8 515,391 + ybbL b0490 0.37 -2.1 515,391 + ybbL b0490 0.37 -0.3 515,399 - ybbL b0490 0.38 -1.3 515,399 - ybbL b0490 0.38 +0.8 515,400 + ybbL b0490 0.38 -1.6 515,400 + ybbL b0490 0.38 -1.2 515,402 + ybbL b0490 0.38 +2.0 515,408 - ybbL b0490 0.39 -0.4 515,410 - ybbL b0490 0.39 +0.4 515,413 - ybbL b0490 0.40 +0.1 515,414 - ybbL b0490 0.40 +0.8 515,431 + ybbL b0490 0.42 +0.2 515,434 - ybbL b0490 0.43 +1.3 515,439 - ybbL b0490 0.44 -1.6 515,450 + ybbL b0490 0.45 -1.0 515,473 + ybbL b0490 0.49 -2.7 515,474 + ybbL b0490 0.49 -1.6 515,474 + ybbL b0490 0.49 -0.7 515,549 - ybbL b0490 0.60 +3.6 515,553 + ybbL b0490 0.60 +0.3 515,661 + ybbL b0490 0.76 -0.3 515,661 + ybbL b0490 0.76 -2.2 515,710 + ybbL b0490 0.84 -0.6 515,715 + ybbL b0490 0.84 -0.3 515,718 - ybbL b0490 0.85 +3.6 515,718 - ybbL b0490 0.85 +3.6 515,754 + +2.0 515,768 + -1.6 515,768 + -0.0 515,770 + -1.6 515,783 + -0.5 515,783 + -0.9 515,803 + -0.4 515,803 + -0.9 515,845 + +0.1 515,846 + -0.9 515,847 + +0.3 515,847 + +0.4 515,847 + -0.8 515,847 + +0.7 515,852 + +1.8 515,852 + -0.3 515,853 + +1.0 515,853 + +0.9 515,872 + +0.5 515,907 + ybbM b0491 0.13 +0.3 515,947 + ybbM b0491 0.18 -1.5 515,982 + ybbM b0491 0.22 +2.3 515,987 + ybbM b0491 0.23 -1.6 515,991 + ybbM b0491 0.24 +1.0 515,991 + ybbM b0491 0.24 +2.0 516,009 - ybbM b0491 0.26 +2.0 516,010 - ybbM b0491 0.26 +3.6 516,010 - ybbM b0491 0.26 +3.6 516,118 + ybbM b0491 0.40 +0.6 516,118 + ybbM b0491 0.40 +0.3 516,120 + ybbM b0491 0.40 -1.0 516,122 + ybbM b0491 0.40 -1.3 516,122 + ybbM b0491 0.40 +2.3 516,127 + ybbM b0491 0.41 -1.3 516,141 + ybbM b0491 0.43 +0.3 516,144 - ybbM b0491 0.43 +1.3 516,144 - ybbM b0491 0.43 +2.6 516,149 - ybbM b0491 0.44 -0.4 516,149 - ybbM b0491 0.44 -0.2 516,149 - ybbM b0491 0.44 +2.0 516,150 - ybbM b0491 0.44 -0.4 516,150 - ybbM b0491 0.44 +2.6 516,202 - ybbM b0491 0.51 +1.0 516,213 - ybbM b0491 0.52 -0.2 516,216 + ybbM b0491 0.52 +1.0 516,216 + ybbM b0491 0.52 +0.9 516,217 - ybbM b0491 0.53 -0.1 516,224 + ybbM b0491 0.53 -1.2 516,245 - ybbM b0491 0.56 +1.7 516,265 + ybbM b0491 0.59 -0.5 516,310 + ybbM b0491 0.64 -2.8 516,310 + ybbM b0491 0.64 -0.5 516,313 - ybbM b0491 0.65 +2.0 516,313 - ybbM b0491 0.65 +0.1 516,331 + ybbM b0491 0.67 +1.6 516,393 + ybbM b0491 0.75 +3.6 516,393 + ybbM b0491 0.75 -1.2 516,393 + ybbM b0491 0.75 -2.3 516,404 - ybbM b0491 0.77 -1.2 516,414 + ybbM b0491 0.78 -0.7 516,414 + ybbM b0491 0.78 -1.3 516,473 + ybbM b0491 0.85 +0.5 516,479 - ybbM b0491 0.86 -0.2 516,489 - ybbM b0491 0.87 +0.6 516,634 - -0.0 516,636 + -0.2 516,636 + -3.1 516,644 + -0.6 516,667 + -0.9 516,667 + -2.4 516,670 - +0.1 516,707 - -1.9 516,824 - ybbN b0492 0.20 -1.8 516,922 + ybbN b0492 0.32 +0.3 516,972 - ybbN b0492 0.38 +0.2 516,972 - ybbN b0492 0.38 -0.2 516,986 + ybbN b0492 0.39 +1.3 517,018 + ybbN b0492 0.43 +0.3 517,026 - ybbN b0492 0.44 +0.8 517,026 - ybbN b0492 0.44 -0.2 517,096 - ybbN b0492 0.52 -0.1 517,096 - ybbN b0492 0.52 -0.6 517,181 + ybbN b0492 0.62 +0.8 517,255 + ybbN b0492 0.71 -0.8 517,308 - ybbN b0492 0.77 -1.4 517,308 - ybbN b0492 0.77 -1.8 517,340 - ybbN b0492 0.81 -1.4 517,345 + ybbN b0492 0.81 -0.1 517,401 - ybbN b0492 0.88 -0.7 517,433 - -0.9 517,444 - +0.2
Or see this region's nucleotide sequence