Strain Fitness in Escherichia coli BW25113 around b0368

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttauB and tauC overlap by 4 nucleotidestauC and tauD overlap by 4 nucleotidestauD and hemB are separated by 106 nucleotides b0366: tauB - taurine transporter subunit (NCBI), at 385,431 to 386,198 tauB b0367: tauC - taurine transporter subunit (NCBI), at 386,195 to 387,022 tauC b0368: tauD - taurine dioxygenase (NCBI), at 387,019 to 387,870 tauD b0369: hemB - 5-aminolevulinate dehydratase = porphobilinogen synthase (VIMSS), at 387,977 to 388,951 hemB Position (kb) 387 388Strain fitness (log2 ratio) -2 -1 0 1 2at 386.055 kb on + strand, within tauBat 386.094 kb on + strand, within tauBat 386.245 kb on - strandat 386.275 kb on + strandat 386.297 kb on - strand, within tauCat 386.297 kb on - strand, within tauCat 386.532 kb on + strand, within tauCat 386.532 kb on + strand, within tauCat 386.562 kb on + strand, within tauCat 386.586 kb on - strand, within tauCat 386.729 kb on + strand, within tauCat 386.829 kb on - strand, within tauCat 386.958 kb on + strandat 387.043 kb on - strandat 387.266 kb on + strand, within tauDat 387.266 kb on + strand, within tauDat 387.410 kb on + strand, within tauDat 387.622 kb on + strand, within tauDat 387.649 kb on + strand, within tauDat 387.649 kb on + strand, within tauDat 387.669 kb on - strand, within tauDat 387.678 kb on + strand, within tauDat 387.775 kb on + strand, within tauDat 387.775 kb on + strand, within tauDat 387.783 kb on - strand, within tauDat 387.896 kb on + strandat 387.908 kb on + strandat 387.908 kb on + strandat 387.916 kb on - strandat 387.916 kb on - strandat 387.916 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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386,055 + tauB b0366 0.81 -2.0
386,094 + tauB b0366 0.86 -1.2
386,245 - -0.3
386,275 + -1.8
386,297 - tauC b0367 0.12 +2.5
386,297 - tauC b0367 0.12 +1.5
386,532 + tauC b0367 0.41 -0.5
386,532 + tauC b0367 0.41 -1.3
386,562 + tauC b0367 0.44 -1.1
386,586 - tauC b0367 0.47 +0.4
386,729 + tauC b0367 0.64 -0.5
386,829 - tauC b0367 0.77 +0.2
386,958 + -0.9
387,043 - -1.0
387,266 + tauD b0368 0.29 -0.5
387,266 + tauD b0368 0.29 -2.2
387,410 + tauD b0368 0.46 -0.7
387,622 + tauD b0368 0.71 -0.5
387,649 + tauD b0368 0.74 +1.2
387,649 + tauD b0368 0.74 -1.2
387,669 - tauD b0368 0.76 -2.0
387,678 + tauD b0368 0.77 -0.3
387,775 + tauD b0368 0.89 +1.0
387,775 + tauD b0368 0.89 -1.6
387,783 - tauD b0368 0.90 -1.0
387,896 + -0.5
387,908 + -0.1
387,908 + -0.8
387,916 - -0.6
387,916 - +0.9
387,916 - +0.2

Or see this region's nucleotide sequence