Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafS and rnhA overlap by 4 nucleotides rnhA and dnaQ are separated by 64 nucleotides dnaQ and aspV are separated by 132 nucleotides aspV and yafT are separated by 327 nucleotides
b0213: yafS - orf, hypothetical protein (VIMSS), at 234,816 to 235,538
yafS
b0214: rnhA - ribonuclease H (NCBI), at 235,535 to 236,002
rnhA
b0215: dnaQ - DNA polymerase III subunit epsilon (NCBI), at 236,067 to 236,798
dnaQ
b0216: aspV - tRNA-Asp (NCBI), at 236,931 to 237,007
aspV
b0217: yafT - predicted aminopeptidase (NCBI), at 237,335 to 238,120
yafT
Position (kb) 236
237 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 235.257 kb on + strand, within yafS at 235.257 kb on + strand, within yafS at 235.325 kb on + strand, within yafS at 235.333 kb on - strand, within yafS at 235.379 kb on + strand, within yafS at 235.519 kb on + strand at 235.527 kb on - strand at 235.569 kb on - strand at 235.569 kb on - strand at 236.069 kb on + strand at 236.072 kb on - strand at 236.876 kb on + strand at 236.876 kb on + strand at 236.910 kb on + strand at 236.910 kb on + strand at 236.917 kb on + strand at 236.965 kb on + strand, within aspV at 236.965 kb on + strand, within aspV at 236.968 kb on + strand, within aspV at 236.985 kb on + strand, within aspV at 236.985 kb on + strand, within aspV at 236.993 kb on + strand, within aspV at 236.994 kb on + strand, within aspV at 237.008 kb on + strand at 237.008 kb on + strand at 237.027 kb on + strand at 237.027 kb on + strand at 237.110 kb on + strand at 237.110 kb on + strand at 237.122 kb on + strand at 237.131 kb on + strand at 237.163 kb on + strand at 237.576 kb on - strand, within yafT at 237.576 kb on - strand, within yafT at 237.619 kb on - strand, within yafT at 237.669 kb on - strand, within yafT at 237.674 kb on - strand, within yafT at 237.687 kb on + strand, within yafT at 237.689 kb on - strand, within yafT at 237.689 kb on - strand, within yafT at 237.741 kb on + strand, within yafT at 237.741 kb on + strand, within yafT at 237.775 kb on + strand, within yafT
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 235,257 + yafS b0213 0.61 -0.4 235,257 + yafS b0213 0.61 -1.9 235,325 + yafS b0213 0.70 +1.1 235,333 - yafS b0213 0.72 +0.3 235,379 + yafS b0213 0.78 -1.3 235,519 + -0.4 235,527 - -1.0 235,569 - +3.5 235,569 - +0.2 236,069 + +0.1 236,072 - -1.8 236,876 + -1.5 236,876 + -0.7 236,910 + +0.1 236,910 + -0.6 236,917 + +1.5 236,965 + aspV b0216 0.44 +1.4 236,965 + aspV b0216 0.44 +0.3 236,968 + aspV b0216 0.48 +0.7 236,985 + aspV b0216 0.70 +2.4 236,985 + aspV b0216 0.70 +1.4 236,993 + aspV b0216 0.81 +3.3 236,994 + aspV b0216 0.82 +2.8 237,008 + +1.1 237,008 + +0.2 237,027 + +3.3 237,027 + +4.2 237,110 + -1.0 237,110 + -2.2 237,122 + -0.5 237,131 + -0.2 237,163 + +0.1 237,576 - yafT b0217 0.31 +3.5 237,576 - yafT b0217 0.31 +3.5 237,619 - yafT b0217 0.36 +3.5 237,669 - yafT b0217 0.42 +3.5 237,674 - yafT b0217 0.43 +3.5 237,687 + yafT b0217 0.45 +3.5 237,689 - yafT b0217 0.45 +3.5 237,689 - yafT b0217 0.45 +2.5 237,741 + yafT b0217 0.52 +3.5 237,741 + yafT b0217 0.52 +3.5 237,775 + yafT b0217 0.56 +0.9
Or see this region's nucleotide sequence