Strain Fitness in Escherichia coli BW25113 around b0094

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntddlB and ftsQ are separated by 1 nucleotidesftsQ and ftsA overlap by 4 nucleotidesftsA and ftsZ are separated by 60 nucleotides b0092: ddlB - D-alanylalanine synthetase (NCBI), at 102,233 to 103,153 ddlB b0093: ftsQ - membrane anchored protein involved in growth of wall at septum (NCBI), at 103,155 to 103,985 ftsQ b0094: ftsA - cell division protein (NCBI), at 103,982 to 105,244 ftsA b0095: ftsZ - cell division protein FtsZ (NCBI), at 105,305 to 106,456 ftsZ Position (kb) 103 104 105 106Strain fitness (log2 ratio) -1 0 1 2 3at 102.984 kb on + strand, within ddlBat 103.002 kb on + strand, within ddlBat 103.017 kb on + strand, within ddlBat 103.068 kb on + strandat 103.138 kb on + strandat 103.911 kb on + strandat 103.911 kb on + strandat 103.916 kb on + strandat 103.916 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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102,984 + ddlB b0092 0.82 +1.7
103,002 + ddlB b0092 0.83 +1.3
103,017 + ddlB b0092 0.85 +0.8
103,068 + +0.9
103,138 + +2.9
103,911 + -0.6
103,911 + +0.1
103,916 + +0.0
103,916 + +1.9

Or see this region's nucleotide sequence