Strain Fitness in Escherichia coli BW25113 around b0664

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiF and glnX are separated by 153 nucleotidesglnX and glnV are separated by 37 nucleotidesglnV and metU are separated by 47 nucleotidesmetU and glnW are separated by 15 nucleotidesglnW and glnU are separated by 34 nucleotidesglnU and leuW are separated by 23 nucleotidesleuW and metT are separated by 9 nucleotides b0662: ubiF - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (NCBI), at 694,324 to 695,499 ubiF b0664: glnX - tRNA-Gln (NCBI), at 695,653 to 695,727 glnX b0665: glnV - tRNA-Gln (NCBI), at 695,765 to 695,839 glnV b0666: metU - tRNA-Met (NCBI), at 695,887 to 695,963 metU b0668: glnW - tRNA-Gln (NCBI), at 695,979 to 696,053 glnW b0670: glnU - tRNA-Gln (NCBI), at 696,088 to 696,162 glnU b0672: leuW - tRNA-Leu (NCBI), at 696,186 to 696,270 leuW b0673: metT - tRNA-Met (NCBI), at 696,280 to 696,356 metT Position (kb) 695 696Strain fitness (log2 ratio) -2 -1 0 1 2 3at 695.499 kb on - strandat 695.507 kb on + strandat 695.779 kb on - strand, within glnVat 695.885 kb on - strandat 695.885 kb on - strandat 695.919 kb on - strand, within metUat 696.470 kb on + strandat 696.513 kb on - strandat 696.513 kb on - strandat 696.625 kb on + strandat 696.625 kb on + strandat 696.630 kb on - strandat 696.639 kb on - strandat 696.639 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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695,499 - -0.1
695,507 + -2.7
695,779 - glnV b0665 0.19 +1.9
695,885 - +1.2
695,885 - +2.1
695,919 - metU b0666 0.42 +3.6
696,470 + -0.6
696,513 - -0.6
696,513 - -1.3
696,625 + +0.7
696,625 + -1.3
696,630 - -1.1
696,639 - +0.3
696,639 - -0.4

Or see this region's nucleotide sequence