Strain Fitness in Escherichia coli BW25113 around b3581

Experiment: vcDART vector transformation; liquid outgrowth assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; liquid outgrowth assay
3,745,629	-	lyxK	b3580	0.35	-1.1
3,745,652	+	lyxK	b3580	0.36	+0.8
3,745,666	+	lyxK	b3580	0.37	-0.7
3,745,712	-	lyxK	b3580	0.40	-2.5
3,745,727	+	lyxK	b3580	0.41	-1.2
3,745,728	+	lyxK	b3580	0.41	-0.9
3,745,767	-	lyxK	b3580	0.44	+0.2
3,745,785	+	lyxK	b3580	0.45	+0.9
3,745,881	-	lyxK	b3580	0.52	-1.5
3,745,881	-	lyxK	b3580	0.52	+0.6
3,745,900	-	lyxK	b3580	0.53	+0.3
3,745,973	+	lyxK	b3580	0.58	-2.3
3,745,973	+	lyxK	b3580	0.58	+0.1
3,746,056	-	lyxK	b3580	0.63	-0.7
3,746,056	-	lyxK	b3580	0.63	-0.4
3,746,070	+	lyxK	b3580	0.64	+1.0
3,746,086	+	lyxK	b3580	0.65	-1.2
3,746,133	+	lyxK	b3580	0.69	+0.6
3,746,138	-	lyxK	b3580	0.69	+0.1
3,746,181	+	lyxK	b3580	0.72	-0.2
3,746,181	+	lyxK	b3580	0.72	-2.6
3,746,198	-	lyxK	b3580	0.73	-0.5
3,746,198	-	lyxK	b3580	0.73	+2.0
3,746,286	+	lyxK	b3580	0.79	+1.4
3,746,293	+	lyxK	b3580	0.79	-0.6
3,746,295	-	lyxK	b3580	0.79	-0.3
3,746,295	-	lyxK	b3580	0.79	-0.0
3,746,349	+	lyxK	b3580	0.83	-0.2
3,746,349	+	lyxK	b3580	0.83	-0.8
3,746,399	+	lyxK	b3580	0.86	+0.8
3,746,399	+	lyxK	b3580	0.86	+0.5
3,746,423	+	lyxK	b3580	0.88	-0.9
3,746,457	+				-0.4
3,746,562	-				-0.2
3,746,570	-				-2.1
3,746,581	+				-0.5
3,746,594	+				+0.3
3,746,594	+				-0.5
3,746,596	+				+0.1
3,746,641	+				-0.6
3,746,643	-				-0.1
3,746,726	+	sgbH	b3581	0.19	+0.2
3,746,726	+	sgbH	b3581	0.19	-0.0
3,746,800	-	sgbH	b3581	0.30	-0.2
3,746,839	+	sgbH	b3581	0.36	-0.3
3,746,852	+	sgbH	b3581	0.38	+0.8
3,746,852	+	sgbH	b3581	0.38	+0.5
3,746,860	-	sgbH	b3581	0.39	-1.6
3,746,928	+	sgbH	b3581	0.49	-1.0
3,746,936	-	sgbH	b3581	0.51	-0.8
3,746,936	-	sgbH	b3581	0.51	+0.5
3,746,959	-	sgbH	b3581	0.54	-0.1
3,746,978	-	sgbH	b3581	0.57	+0.9
3,747,007	-	sgbH	b3581	0.61	-0.8
3,747,117	+	sgbH	b3581	0.78	+2.3
3,747,249	+				+2.4
3,747,600	+	sgbU	b3582	0.40	+1.1
3,747,626	+	sgbU	b3582	0.43	+0.5
3,747,694	+	sgbU	b3582	0.51	-0.4
3,747,819	-	sgbU	b3582	0.66	-2.2
3,747,881	+	sgbU	b3582	0.73	-0.5
3,747,923	+	sgbU	b3582	0.78	+0.3
3,748,022	-	sgbU	b3582	0.89	+0.1
3,748,022	-	sgbU	b3582	0.89	-0.8
3,748,034	+				+0.6
3,748,083	-				-1.4
3,748,103	+				-0.4
3,748,128	-				+0.7
3,748,226	-	sgbE	b3583	0.17	+0.4
3,748,237	+	sgbE	b3583	0.18	+0.4
3,748,237	+	sgbE	b3583	0.18	+1.8
3,748,253	+	sgbE	b3583	0.21	-0.6