Strain Fitness in Escherichia coli BW25113 around b2307

Experiment: vcDART vector transformation; liquid outgrowth assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfcI and hisP are separated by 196 nucleotideshisP and hisM are separated by 7 nucleotideshisM and hisQ overlap by 4 nucleotideshisQ and hisJ are separated by 89 nucleotides b2305: yfcI - hypothetical protein (NCBI), at 2,420,671 to 2,421,561 yfcI b2306: hisP - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,421,758 to 2,422,531 hisP b2307: hisM - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,422,539 to 2,423,255 hisM b2308: hisQ - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,423,252 to 2,423,938 hisQ b2309: hisJ - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,424,028 to 2,424,810 hisJ Position (kb) 2422 2423 2424Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2421.694 kb on + strandat 2421.771 kb on + strandat 2421.771 kb on + strandat 2421.782 kb on - strandat 2421.790 kb on - strandat 2421.793 kb on - strandat 2421.793 kb on - strandat 2421.793 kb on - strandat 2421.793 kb on - strandat 2421.806 kb on - strandat 2421.860 kb on - strand, within hisPat 2421.874 kb on - strand, within hisPat 2421.918 kb on - strand, within hisPat 2421.921 kb on + strand, within hisPat 2421.928 kb on - strand, within hisPat 2421.928 kb on - strand, within hisPat 2421.963 kb on + strand, within hisPat 2421.963 kb on + strand, within hisPat 2421.971 kb on - strand, within hisPat 2421.973 kb on + strand, within hisPat 2421.981 kb on - strand, within hisPat 2421.981 kb on - strand, within hisPat 2422.005 kb on - strand, within hisPat 2422.199 kb on + strand, within hisPat 2422.199 kb on + strand, within hisPat 2422.218 kb on - strand, within hisPat 2422.232 kb on + strand, within hisPat 2422.232 kb on + strand, within hisPat 2422.255 kb on - strand, within hisPat 2422.255 kb on - strand, within hisPat 2422.375 kb on + strand, within hisPat 2422.486 kb on - strandat 2422.538 kb on - strandat 2422.538 kb on - strandat 2422.607 kb on + strandat 2422.607 kb on + strandat 2422.607 kb on + strandat 2422.621 kb on + strand, within hisMat 2422.627 kb on - strand, within hisMat 2422.684 kb on - strand, within hisMat 2422.684 kb on - strand, within hisMat 2422.684 kb on - strand, within hisMat 2422.711 kb on - strand, within hisMat 2422.712 kb on - strand, within hisMat 2422.730 kb on + strand, within hisMat 2422.738 kb on - strand, within hisMat 2422.740 kb on + strand, within hisMat 2422.740 kb on + strand, within hisMat 2422.765 kb on + strand, within hisMat 2422.765 kb on - strand, within hisMat 2422.788 kb on - strand, within hisMat 2422.798 kb on - strand, within hisMat 2422.836 kb on + strand, within hisMat 2422.850 kb on - strand, within hisMat 2422.850 kb on - strand, within hisMat 2422.904 kb on - strand, within hisMat 2422.942 kb on + strand, within hisMat 2422.947 kb on - strand, within hisMat 2422.962 kb on - strand, within hisMat 2423.022 kb on + strand, within hisMat 2423.038 kb on + strand, within hisMat 2423.041 kb on - strand, within hisMat 2423.145 kb on + strand, within hisMat 2423.145 kb on + strand, within hisMat 2423.199 kb on - strandat 2423.199 kb on - strandat 2423.233 kb on - strandat 2423.240 kb on - strandat 2423.240 kb on - strandat 2423.265 kb on - strandat 2423.265 kb on - strandat 2423.267 kb on + strandat 2423.280 kb on + strandat 2423.280 kb on + strandat 2423.310 kb on - strandat 2423.310 kb on - strandat 2423.346 kb on + strand, within hisQat 2423.421 kb on - strand, within hisQat 2423.426 kb on + strand, within hisQat 2423.490 kb on + strand, within hisQat 2423.566 kb on + strand, within hisQat 2423.572 kb on - strand, within hisQat 2423.641 kb on + strand, within hisQat 2423.670 kb on + strand, within hisQat 2423.696 kb on + strand, within hisQat 2423.696 kb on + strand, within hisQat 2423.702 kb on - strand, within hisQat 2423.702 kb on - strand, within hisQat 2423.752 kb on - strand, within hisQat 2423.779 kb on + strand, within hisQat 2423.786 kb on - strand, within hisQat 2423.830 kb on - strand, within hisQat 2423.830 kb on - strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.940 kb on + strandat 2424.014 kb on - strandat 2424.158 kb on - strand, within hisJat 2424.158 kb on - strand, within hisJat 2424.158 kb on - strand, within hisJat 2424.239 kb on - strand, within hisJ

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Per-strain Table

Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay
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2,421,694 + +0.9
2,421,771 + +1.7
2,421,771 + +2.1
2,421,782 - -1.1
2,421,790 - +0.3
2,421,793 - +0.5
2,421,793 - +0.1
2,421,793 - -1.2
2,421,793 - -0.3
2,421,806 - -1.2
2,421,860 - hisP b2306 0.13 +2.4
2,421,874 - hisP b2306 0.15 -0.6
2,421,918 - hisP b2306 0.21 -0.3
2,421,921 + hisP b2306 0.21 +3.7
2,421,928 - hisP b2306 0.22 -0.0
2,421,928 - hisP b2306 0.22 +0.4
2,421,963 + hisP b2306 0.26 -0.6
2,421,963 + hisP b2306 0.26 +1.4
2,421,971 - hisP b2306 0.28 -0.8
2,421,973 + hisP b2306 0.28 +3.7
2,421,981 - hisP b2306 0.29 -0.7
2,421,981 - hisP b2306 0.29 +0.1
2,422,005 - hisP b2306 0.32 -0.7
2,422,199 + hisP b2306 0.57 +0.2
2,422,199 + hisP b2306 0.57 +0.3
2,422,218 - hisP b2306 0.59 -0.2
2,422,232 + hisP b2306 0.61 -0.4
2,422,232 + hisP b2306 0.61 +2.1
2,422,255 - hisP b2306 0.64 -0.6
2,422,255 - hisP b2306 0.64 -1.6
2,422,375 + hisP b2306 0.80 -0.0
2,422,486 - +0.2
2,422,538 - +0.6
2,422,538 - -2.6
2,422,607 + -0.1
2,422,607 + -1.8
2,422,607 + +3.0
2,422,621 + hisM b2307 0.11 +0.9
2,422,627 - hisM b2307 0.12 -1.9
2,422,684 - hisM b2307 0.20 +1.4
2,422,684 - hisM b2307 0.20 -0.2
2,422,684 - hisM b2307 0.20 -1.7
2,422,711 - hisM b2307 0.24 +0.2
2,422,712 - hisM b2307 0.24 -2.4
2,422,730 + hisM b2307 0.27 -2.3
2,422,738 - hisM b2307 0.28 +0.7
2,422,740 + hisM b2307 0.28 -0.5
2,422,740 + hisM b2307 0.28 -0.7
2,422,765 + hisM b2307 0.32 -2.4
2,422,765 - hisM b2307 0.32 -1.3
2,422,788 - hisM b2307 0.35 -2.1
2,422,798 - hisM b2307 0.36 -0.6
2,422,836 + hisM b2307 0.41 -1.2
2,422,850 - hisM b2307 0.43 -1.4
2,422,850 - hisM b2307 0.43 -1.5
2,422,904 - hisM b2307 0.51 -0.2
2,422,942 + hisM b2307 0.56 -1.1
2,422,947 - hisM b2307 0.57 -0.6
2,422,962 - hisM b2307 0.59 -2.2
2,423,022 + hisM b2307 0.67 -1.2
2,423,038 + hisM b2307 0.70 -1.3
2,423,041 - hisM b2307 0.70 -0.6
2,423,145 + hisM b2307 0.85 +0.7
2,423,145 + hisM b2307 0.85 -0.3
2,423,199 - -0.1
2,423,199 - -0.6
2,423,233 - -0.1
2,423,240 - -1.6
2,423,240 - +0.6
2,423,265 - -1.4
2,423,265 - -0.7
2,423,267 + -1.3
2,423,280 + +0.1
2,423,280 + -1.7
2,423,310 - -0.6
2,423,310 - -0.6
2,423,346 + hisQ b2308 0.14 -0.7
2,423,421 - hisQ b2308 0.25 -1.2
2,423,426 + hisQ b2308 0.25 +0.7
2,423,490 + hisQ b2308 0.35 -1.8
2,423,566 + hisQ b2308 0.46 +0.7
2,423,572 - hisQ b2308 0.47 -0.7
2,423,641 + hisQ b2308 0.57 -0.4
2,423,670 + hisQ b2308 0.61 -0.8
2,423,696 + hisQ b2308 0.65 -0.1
2,423,696 + hisQ b2308 0.65 -0.3
2,423,702 - hisQ b2308 0.66 -0.4
2,423,702 - hisQ b2308 0.66 -2.0
2,423,752 - hisQ b2308 0.73 -0.8
2,423,779 + hisQ b2308 0.77 -1.0
2,423,786 - hisQ b2308 0.78 -1.1
2,423,830 - hisQ b2308 0.84 -0.3
2,423,830 - hisQ b2308 0.84 +0.7
2,423,866 + hisQ b2308 0.89 -1.6
2,423,866 + hisQ b2308 0.89 -0.4
2,423,866 + hisQ b2308 0.89 +0.5
2,423,866 + hisQ b2308 0.89 +1.1
2,423,866 + hisQ b2308 0.89 +0.2
2,423,940 + -0.8
2,424,014 - -0.3
2,424,158 - hisJ b2309 0.17 +1.4
2,424,158 - hisJ b2309 0.17 -0.7
2,424,158 - hisJ b2309 0.17 -2.4
2,424,239 - hisJ b2309 0.27 -0.7

Or see this region's nucleotide sequence