Experiment: vcDART vector transformation; liquid outgrowth assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydaJ and abgR are separated by 175 nucleotides abgR and isrA are separated by 2 nucleotides isrA and ydaL are separated by 169 nucleotides ydaL and b1341 are separated by 20 nucleotides
b1338: ydaJ - putative aminohydrolase (EC 3.5.1.14) (VIMSS), at 1,401,279 to 1,402,589
ydaJ
b1339: abgR - predicted DNA-binding transcriptional regulator (NCBI), at 1,402,765 to 1,403,673
abgR
b4426: isrA - unknown RNA (NCBI), at 1,403,676 to 1,403,833
isrA
b1340: ydaL - hypothetical protein (NCBI), at 1,404,003 to 1,404,566
ydaL
b1341: b1341 - orf, hypothetical protein (VIMSS), at 1,404,587 to 1,405,819
b1341
Position (kb)
1402
1403
1404 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1401.801 kb on - strand, within ydaJ at 1401.801 kb on - strand, within ydaJ at 1401.885 kb on - strand, within ydaJ at 1401.932 kb on + strand, within ydaJ at 1401.932 kb on + strand, within ydaJ at 1402.102 kb on - strand, within ydaJ at 1402.102 kb on - strand, within ydaJ at 1402.268 kb on - strand, within ydaJ at 1402.450 kb on - strand, within ydaJ at 1402.513 kb on + strand at 1402.592 kb on - strand at 1402.592 kb on - strand at 1402.834 kb on - strand at 1402.988 kb on + strand, within abgR at 1403.075 kb on + strand, within abgR at 1403.075 kb on + strand, within abgR at 1403.087 kb on + strand, within abgR at 1403.087 kb on + strand, within abgR at 1403.109 kb on - strand, within abgR at 1403.119 kb on - strand, within abgR at 1403.119 kb on - strand, within abgR at 1403.153 kb on - strand, within abgR at 1403.153 kb on - strand, within abgR at 1403.464 kb on - strand, within abgR at 1403.464 kb on - strand, within abgR at 1403.519 kb on - strand, within abgR at 1403.519 kb on - strand, within abgR at 1403.720 kb on + strand, within isrA at 1403.720 kb on + strand, within isrA at 1403.732 kb on - strand, within isrA at 1403.735 kb on - strand, within isrA at 1404.417 kb on - strand, within ydaL at 1404.648 kb on + strand at 1404.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; liquid outgrowth assay remove 1,401,801 - ydaJ b1338 0.40 -0.8 1,401,801 - ydaJ b1338 0.40 -2.6 1,401,885 - ydaJ b1338 0.46 -1.2 1,401,932 + ydaJ b1338 0.50 -1.9 1,401,932 + ydaJ b1338 0.50 -0.7 1,402,102 - ydaJ b1338 0.63 -1.3 1,402,102 - ydaJ b1338 0.63 -0.2 1,402,268 - ydaJ b1338 0.75 -0.4 1,402,450 - ydaJ b1338 0.89 -0.5 1,402,513 + +0.7 1,402,592 - -0.1 1,402,592 - -0.0 1,402,834 - -1.3 1,402,988 + abgR b1339 0.25 -1.2 1,403,075 + abgR b1339 0.34 +0.6 1,403,075 + abgR b1339 0.34 -1.4 1,403,087 + abgR b1339 0.35 -1.1 1,403,087 + abgR b1339 0.35 -0.2 1,403,109 - abgR b1339 0.38 -2.6 1,403,119 - abgR b1339 0.39 -1.4 1,403,119 - abgR b1339 0.39 -0.8 1,403,153 - abgR b1339 0.43 +0.2 1,403,153 - abgR b1339 0.43 -0.9 1,403,464 - abgR b1339 0.77 -0.7 1,403,464 - abgR b1339 0.77 -0.6 1,403,519 - abgR b1339 0.83 -0.8 1,403,519 - abgR b1339 0.83 -0.6 1,403,720 + isrA b4426 0.28 +0.1 1,403,720 + isrA b4426 0.28 -0.8 1,403,732 - isrA b4426 0.35 +2.4 1,403,735 - isrA b4426 0.37 +1.0 1,404,417 - ydaL b1340 0.73 -0.6 1,404,648 + +0.2 1,404,666 - -0.0
Or see this region's nucleotide sequence