Strain Fitness in Escherichia coli BW25113 around b3023

Experiment: mariner vector transformation; agar assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	mariner vector transformation; agar assay
3,165,775	+				-0.7
3,165,784	-				+0.9
3,165,798	+				+0.5
3,165,798	+				-0.5
3,165,822	+				-0.4
3,165,822	+				-0.5
3,165,895	+				+1.6
3,165,903	+				+1.6
3,165,914	-	ygiT	b3021	0.10	+2.8
3,165,946	-	ygiT	b3021	0.18	+1.6
3,165,950	-	ygiT	b3021	0.19	+0.0
3,165,950	-	ygiT	b3021	0.19	-0.6
3,165,985	-	ygiT	b3021	0.28	-0.7
3,166,018	-	ygiT	b3021	0.37	+0.3
3,166,093	+	ygiT	b3021	0.56	+1.6
3,166,285	-				-0.0
3,166,285	-				-0.6
3,166,286	-				-0.5
3,166,290	-				-0.4
3,166,290	-				-1.6
3,166,299	-				+0.6
3,166,308	-	b3022	b3022	0.13	-0.7
3,166,308	-	b3022	b3022	0.13	-1.8
3,166,325	-	b3022	b3022	0.19	-0.8
3,166,330	-	b3022	b3022	0.20	-0.8
3,166,330	-	b3022	b3022	0.20	-0.5
3,166,335	-	b3022	b3022	0.22	-0.5
3,166,337	+	b3022	b3022	0.23	+2.6
3,166,345	-	b3022	b3022	0.25	+0.1
3,166,345	-	b3022	b3022	0.25	-1.2
3,166,346	-	b3022	b3022	0.26	-0.8
3,166,357	-	b3022	b3022	0.29	-0.6
3,166,370	+	b3022	b3022	0.34	-1.4
3,166,376	-	b3022	b3022	0.36	-0.6
3,166,377	+	b3022	b3022	0.36	-0.4
3,166,382	+	b3022	b3022	0.38	-0.6
3,166,382	+	b3022	b3022	0.38	+0.3
3,166,388	-	b3022	b3022	0.40	-0.2
3,166,388	-	b3022	b3022	0.40	-0.1
3,166,390	-	b3022	b3022	0.40	-1.1
3,166,420	-	b3022	b3022	0.51	-1.7
3,166,441	-	b3022	b3022	0.58	-0.4
3,166,480	-	b3022	b3022	0.71	-0.8
3,166,480	-	b3022	b3022	0.71	-1.2
3,166,523	+	b3022	b3022	0.85	-0.4
3,166,539	+				-0.6
3,166,551	-				-0.1
3,166,551	-				+0.5
3,166,551	-				+0.1
3,166,576	-				-1.1
3,166,576	-				+0.2
3,166,696	-				-0.2
3,166,703	+				-0.4
3,166,703	+				-1.8
3,166,771	-				-0.3
3,166,795	-				-0.3
3,166,795	-				+1.4
3,166,843	+	ygiV	b3023	0.15	-0.2
3,166,909	-	ygiV	b3023	0.29	-0.6
3,166,956	-	ygiV	b3023	0.38	+0.6
3,166,956	-	ygiV	b3023	0.38	-0.1
3,166,989	+	ygiV	b3023	0.45	-0.2
3,166,989	+	ygiV	b3023	0.45	-0.3
3,167,060	+	ygiV	b3023	0.60	-0.2
3,167,154	+	ygiV	b3023	0.79	-0.9
3,167,158	+	ygiV	b3023	0.80	-0.3
3,167,175	+	ygiV	b3023	0.84	-0.9
3,167,175	+	ygiV	b3023	0.84	-0.4
3,167,175	+	ygiV	b3023	0.84	-0.4
3,167,192	+	ygiV	b3023	0.87	-0.1
3,167,227	+				-0.1
3,167,422	-	ygiW	b3024	0.30	+1.6
3,167,470	+	ygiW	b3024	0.42	-0.3
3,167,470	+	ygiW	b3024	0.42	-0.2
3,167,470	+	ygiW	b3024	0.42	-2.2
3,167,478	-	ygiW	b3024	0.44	-0.2
3,167,478	-	ygiW	b3024	0.44	-0.5
3,167,500	-	ygiW	b3024	0.49	+0.4
3,167,500	-	ygiW	b3024	0.49	+0.6
3,167,512	+	ygiW	b3024	0.52	-1.1
3,167,652	+	ygiW	b3024	0.88	-0.6
3,167,718	-				+0.3
3,167,793	-				-0.0
3,167,793	-				-0.6
3,167,798	+				-0.0
3,167,800	+				+0.2
3,167,800	+				-0.4
3,167,800	+				-0.3
3,167,979	+	qseB	b3025	0.20	+0.7
3,167,987	-	qseB	b3025	0.21	-0.9
3,167,987	-	qseB	b3025	0.21	+0.1
3,168,014	-	qseB	b3025	0.25	-0.0
3,168,014	-	qseB	b3025	0.25	-0.9
3,168,045	+	qseB	b3025	0.30	+0.1
3,168,096	+	qseB	b3025	0.37	-0.0
3,168,108	+	qseB	b3025	0.39	-1.1
3,168,112	+	qseB	b3025	0.40	+0.0
3,168,179	-	qseB	b3025	0.50	-0.1
3,168,192	+	qseB	b3025	0.52	-0.2
3,168,235	+	qseB	b3025	0.58	-0.3