Experiment: mariner vector transformation; agar assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rihA and gltL are separated by 117 nucleotides gltL and gltK overlap by 1 nucleotides gltK and gltJ overlap by 1 nucleotides gltJ and gltI are separated by 169 nucleotides
b0651: rihA - ribonucleoside hydrolase 1 (NCBI), at 682,700 to 683,635
rihA
b0652: gltL - glutamate and aspartate transporter subunit (NCBI), at 683,753 to 684,478
gltL
b0653: gltK - glutamate and aspartate transporter subunit (NCBI), at 684,478 to 685,152
gltK
b0654: gltJ - glutamate and aspartate transporter subunit (NCBI), at 685,152 to 685,892
gltJ
b0655: gltI - glutamate and aspartate transporter subunit (NCBI), at 686,062 to 686,970
gltI
Position (kb) 684
685
686 Strain fitness (log2 ratio) -1
0
1 at 683.570 kb on + strand at 683.575 kb on + strand at 683.595 kb on + strand at 683.629 kb on + strand at 683.741 kb on - strand at 683.759 kb on - strand at 683.810 kb on - strand at 684.018 kb on + strand, within gltL at 684.018 kb on + strand, within gltL at 684.026 kb on - strand, within gltL at 684.026 kb on - strand, within gltL at 684.222 kb on + strand, within gltL at 684.225 kb on - strand, within gltL at 684.225 kb on - strand, within gltL at 684.414 kb on + strand at 684.414 kb on + strand at 684.519 kb on + strand at 684.571 kb on - strand, within gltK at 684.631 kb on + strand, within gltK at 684.633 kb on - strand, within gltK at 684.650 kb on + strand, within gltK at 684.650 kb on + strand, within gltK at 684.658 kb on - strand, within gltK at 684.658 kb on - strand, within gltK at 684.660 kb on + strand, within gltK at 684.679 kb on - strand, within gltK at 684.679 kb on - strand, within gltK at 684.731 kb on - strand, within gltK at 684.818 kb on - strand, within gltK at 685.049 kb on + strand, within gltK at 685.049 kb on + strand, within gltK at 685.128 kb on - strand at 685.273 kb on - strand, within gltJ at 685.314 kb on + strand, within gltJ at 685.314 kb on + strand, within gltJ at 685.367 kb on + strand, within gltJ at 685.421 kb on + strand, within gltJ at 685.567 kb on + strand, within gltJ at 685.567 kb on + strand, within gltJ at 685.588 kb on + strand, within gltJ at 685.603 kb on + strand, within gltJ at 685.603 kb on + strand, within gltJ at 685.687 kb on + strand, within gltJ at 685.687 kb on + strand, within gltJ at 685.695 kb on - strand, within gltJ at 685.695 kb on - strand, within gltJ at 685.701 kb on - strand, within gltJ at 685.737 kb on - strand, within gltJ at 685.737 kb on - strand, within gltJ at 685.741 kb on - strand, within gltJ at 685.831 kb on + strand at 685.831 kb on - strand at 685.831 kb on - strand at 685.883 kb on - strand at 685.945 kb on + strand at 685.958 kb on - strand at 685.965 kb on - strand at 685.965 kb on - strand at 685.995 kb on + strand at 685.995 kb on + strand at 686.001 kb on + strand at 686.001 kb on + strand at 686.022 kb on - strand at 686.029 kb on - strand at 686.029 kb on - strand at 686.060 kb on + strand at 686.060 kb on + strand at 686.089 kb on + strand at 686.105 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; agar assay remove 683,570 + -0.4 683,575 + -0.2 683,595 + -0.0 683,629 + -0.6 683,741 - +0.2 683,759 - -0.6 683,810 - -0.3 684,018 + gltL b0652 0.37 +0.0 684,018 + gltL b0652 0.37 -0.6 684,026 - gltL b0652 0.38 -0.3 684,026 - gltL b0652 0.38 +0.3 684,222 + gltL b0652 0.65 -0.2 684,225 - gltL b0652 0.65 -0.7 684,225 - gltL b0652 0.65 -0.3 684,414 + +1.6 684,414 + -1.1 684,519 + -0.5 684,571 - gltK b0653 0.14 -0.0 684,631 + gltK b0653 0.23 -0.8 684,633 - gltK b0653 0.23 -1.5 684,650 + gltK b0653 0.25 -0.1 684,650 + gltK b0653 0.25 -0.5 684,658 - gltK b0653 0.27 -1.3 684,658 - gltK b0653 0.27 +0.2 684,660 + gltK b0653 0.27 -0.7 684,679 - gltK b0653 0.30 -1.1 684,679 - gltK b0653 0.30 -1.2 684,731 - gltK b0653 0.37 -0.3 684,818 - gltK b0653 0.50 -0.3 685,049 + gltK b0653 0.85 -1.5 685,049 + gltK b0653 0.85 -0.1 685,128 - -1.2 685,273 - gltJ b0654 0.16 -0.4 685,314 + gltJ b0654 0.22 -0.3 685,314 + gltJ b0654 0.22 +0.3 685,367 + gltJ b0654 0.29 -0.1 685,421 + gltJ b0654 0.36 -0.4 685,567 + gltJ b0654 0.56 +0.1 685,567 + gltJ b0654 0.56 -0.4 685,588 + gltJ b0654 0.59 -0.1 685,603 + gltJ b0654 0.61 +0.0 685,603 + gltJ b0654 0.61 +0.1 685,687 + gltJ b0654 0.72 -0.4 685,687 + gltJ b0654 0.72 -0.1 685,695 - gltJ b0654 0.73 -0.2 685,695 - gltJ b0654 0.73 -0.7 685,701 - gltJ b0654 0.74 +1.0 685,737 - gltJ b0654 0.79 -0.7 685,737 - gltJ b0654 0.79 +0.7 685,741 - gltJ b0654 0.79 -0.6 685,831 + +0.1 685,831 - -0.3 685,831 - +0.3 685,883 - +0.3 685,945 + -0.1 685,958 - +0.1 685,965 - -1.2 685,965 - -0.3 685,995 + -0.1 685,995 + -0.5 686,001 + -0.2 686,001 + -0.5 686,022 - -0.4 686,029 - -0.3 686,029 - +0.1 686,060 + +0.0 686,060 + -0.2 686,089 + -0.0 686,105 - -0.6
Or see this region's nucleotide sequence