Experiment: mariner vector transformation; agar assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaT and cueR are separated by 124 nucleotides cueR and ybbJ are separated by 0 nucleotides ybbJ and ybbK overlap by 4 nucleotides ybbK and ybbL are separated by 145 nucleotides ybbL and ybbM overlap by 14 nucleotides
b0486: ybaT - predicted transporter (NCBI), at 511,800 to 513,092
ybaT
b0487: cueR - DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI), at 513,217 to 513,624
cueR
b0488: ybbJ - conserved inner membrane protein (RefSeq), at 513,625 to 514,083
ybbJ
b0489: ybbK - predicted protease, membrane anchored (NCBI), at 514,080 to 514,997
ybbK
b0490: ybbL - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 515,143 to 515,820
ybbL
b0491: ybbM - putative metal resistance protein (VIMSS), at 515,807 to 516,586
ybbM
Position (kb) 514
515 Strain fitness (log2 ratio) -2
-1
0
1
2 at 513.095 kb on - strand at 513.107 kb on + strand at 513.109 kb on + strand at 513.182 kb on + strand at 513.205 kb on - strand at 513.287 kb on - strand, within cueR at 513.295 kb on - strand, within cueR at 513.431 kb on - strand, within cueR at 513.480 kb on + strand, within cueR at 513.537 kb on - strand, within cueR at 513.537 kb on - strand, within cueR at 513.700 kb on - strand, within ybbJ at 513.728 kb on - strand, within ybbJ at 513.742 kb on + strand, within ybbJ at 513.742 kb on + strand, within ybbJ at 513.949 kb on - strand, within ybbJ at 513.949 kb on - strand, within ybbJ at 513.958 kb on + strand, within ybbJ at 513.958 kb on + strand, within ybbJ at 513.977 kb on + strand, within ybbJ at 514.116 kb on - strand at 514.116 kb on - strand at 514.118 kb on - strand at 514.118 kb on - strand at 514.129 kb on - strand at 514.133 kb on - strand at 514.133 kb on - strand at 514.142 kb on + strand at 514.142 kb on + strand at 514.185 kb on + strand, within ybbK at 514.473 kb on - strand, within ybbK at 514.546 kb on - strand, within ybbK at 514.636 kb on - strand, within ybbK at 514.645 kb on + strand at 514.653 kb on - strand, within ybbK at 514.653 kb on - strand, within ybbK at 514.662 kb on + strand, within ybbK at 514.697 kb on + strand, within ybbK at 514.806 kb on - strand, within ybbK at 514.851 kb on - strand, within ybbK at 514.853 kb on + strand, within ybbK at 514.853 kb on + strand, within ybbK at 514.863 kb on - strand, within ybbK at 514.863 kb on - strand, within ybbK at 514.907 kb on - strand at 514.907 kb on - strand at 514.959 kb on - strand at 515.010 kb on + strand at 515.018 kb on - strand at 515.018 kb on - strand at 515.018 kb on - strand at 515.020 kb on + strand at 515.020 kb on + strand at 515.020 kb on + strand at 515.021 kb on + strand at 515.021 kb on + strand at 515.055 kb on + strand at 515.184 kb on - strand at 515.187 kb on + strand at 515.313 kb on + strand, within ybbL at 515.313 kb on + strand, within ybbL at 515.332 kb on - strand, within ybbL at 515.344 kb on + strand, within ybbL at 515.360 kb on - strand, within ybbL at 515.360 kb on - strand, within ybbL at 515.373 kb on + strand, within ybbL at 515.385 kb on + strand, within ybbL at 515.391 kb on + strand, within ybbL at 515.391 kb on + strand, within ybbL at 515.399 kb on - strand, within ybbL at 515.399 kb on - strand, within ybbL at 515.400 kb on + strand, within ybbL at 515.400 kb on + strand, within ybbL at 515.402 kb on + strand, within ybbL at 515.408 kb on - strand, within ybbL at 515.410 kb on - strand, within ybbL at 515.413 kb on - strand, within ybbL at 515.414 kb on - strand, within ybbL at 515.431 kb on + strand, within ybbL at 515.434 kb on - strand, within ybbL at 515.439 kb on - strand, within ybbL at 515.450 kb on + strand, within ybbL at 515.473 kb on + strand, within ybbL at 515.474 kb on + strand, within ybbL at 515.474 kb on + strand, within ybbL at 515.549 kb on - strand, within ybbL at 515.553 kb on + strand, within ybbL at 515.661 kb on + strand, within ybbL at 515.661 kb on + strand, within ybbL at 515.710 kb on + strand, within ybbL at 515.715 kb on + strand, within ybbL at 515.718 kb on - strand, within ybbL at 515.718 kb on - strand, within ybbL at 515.754 kb on + strand at 515.768 kb on + strand at 515.768 kb on + strand at 515.770 kb on + strand at 515.783 kb on + strand at 515.783 kb on + strand at 515.803 kb on + strand at 515.803 kb on + strand at 515.845 kb on + strand at 515.846 kb on + strand at 515.847 kb on + strand at 515.847 kb on + strand at 515.847 kb on + strand at 515.847 kb on + strand at 515.852 kb on + strand at 515.852 kb on + strand at 515.853 kb on + strand at 515.853 kb on + strand at 515.872 kb on + strand at 515.907 kb on + strand, within ybbM at 515.947 kb on + strand, within ybbM at 515.982 kb on + strand, within ybbM at 515.987 kb on + strand, within ybbM at 515.991 kb on + strand, within ybbM at 515.991 kb on + strand, within ybbM
Per-strain Table
Position Strand Gene LocusTag Fraction mariner vector transformation; agar assay remove 513,095 - -0.6 513,107 + -0.1 513,109 + -0.6 513,182 + -0.3 513,205 - +0.4 513,287 - cueR b0487 0.17 -0.9 513,295 - cueR b0487 0.19 -0.3 513,431 - cueR b0487 0.52 -0.0 513,480 + cueR b0487 0.64 -0.5 513,537 - cueR b0487 0.78 -0.7 513,537 - cueR b0487 0.78 -0.5 513,700 - ybbJ b0488 0.16 -0.5 513,728 - ybbJ b0488 0.22 -0.0 513,742 + ybbJ b0488 0.25 +1.0 513,742 + ybbJ b0488 0.25 -1.4 513,949 - ybbJ b0488 0.71 -0.2 513,949 - ybbJ b0488 0.71 +1.6 513,958 + ybbJ b0488 0.73 +0.6 513,958 + ybbJ b0488 0.73 +0.3 513,977 + ybbJ b0488 0.77 +0.0 514,116 - -0.2 514,116 - -0.1 514,118 - -1.2 514,118 - +0.9 514,129 - +0.1 514,133 - -0.2 514,133 - -0.0 514,142 + +0.4 514,142 + -0.6 514,185 + ybbK b0489 0.11 -1.1 514,473 - ybbK b0489 0.43 -0.1 514,546 - ybbK b0489 0.51 -0.9 514,636 - ybbK b0489 0.61 -1.0 514,645 + +0.2 514,653 - ybbK b0489 0.62 +0.6 514,653 - ybbK b0489 0.62 -0.2 514,662 + ybbK b0489 0.63 -1.4 514,697 + ybbK b0489 0.67 -1.0 514,806 - ybbK b0489 0.79 -0.1 514,851 - ybbK b0489 0.84 -0.4 514,853 + ybbK b0489 0.84 +0.4 514,853 + ybbK b0489 0.84 -1.2 514,863 - ybbK b0489 0.85 -0.6 514,863 - ybbK b0489 0.85 -0.8 514,907 - -2.3 514,907 - +0.4 514,959 - -0.4 515,010 + -1.5 515,018 - +2.3 515,018 - -0.8 515,018 - +0.5 515,020 + -0.2 515,020 + +0.8 515,020 + +1.2 515,021 + -0.6 515,021 + -0.2 515,055 + +0.2 515,184 - -0.4 515,187 + -0.0 515,313 + ybbL b0490 0.25 +0.8 515,313 + ybbL b0490 0.25 +0.0 515,332 - ybbL b0490 0.28 -1.0 515,344 + ybbL b0490 0.30 -2.0 515,360 - ybbL b0490 0.32 +0.8 515,360 - ybbL b0490 0.32 -1.7 515,373 + ybbL b0490 0.34 -0.5 515,385 + ybbL b0490 0.36 -0.8 515,391 + ybbL b0490 0.37 -2.0 515,391 + ybbL b0490 0.37 -2.2 515,399 - ybbL b0490 0.38 -1.3 515,399 - ybbL b0490 0.38 +0.0 515,400 + ybbL b0490 0.38 +0.2 515,400 + ybbL b0490 0.38 +1.3 515,402 + ybbL b0490 0.38 +2.2 515,408 - ybbL b0490 0.39 -1.6 515,410 - ybbL b0490 0.39 +0.6 515,413 - ybbL b0490 0.40 +1.6 515,414 - ybbL b0490 0.40 +0.5 515,431 + ybbL b0490 0.42 -0.0 515,434 - ybbL b0490 0.43 +0.2 515,439 - ybbL b0490 0.44 -1.9 515,450 + ybbL b0490 0.45 -0.7 515,473 + ybbL b0490 0.49 -0.3 515,474 + ybbL b0490 0.49 +0.1 515,474 + ybbL b0490 0.49 +0.4 515,549 - ybbL b0490 0.60 +1.8 515,553 + ybbL b0490 0.60 +1.2 515,661 + ybbL b0490 0.76 -0.4 515,661 + ybbL b0490 0.76 -0.2 515,710 + ybbL b0490 0.84 -0.6 515,715 + ybbL b0490 0.84 -0.4 515,718 - ybbL b0490 0.85 +1.8 515,718 - ybbL b0490 0.85 +1.8 515,754 + -1.0 515,768 + +0.3 515,768 + +0.6 515,770 + -0.1 515,783 + -0.1 515,783 + +0.3 515,803 + +0.2 515,803 + +0.0 515,845 + -0.5 515,846 + -1.2 515,847 + -1.2 515,847 + -1.9 515,847 + -0.5 515,847 + -0.8 515,852 + -1.8 515,852 + -0.3 515,853 + -0.4 515,853 + +0.7 515,872 + +0.1 515,907 + ybbM b0491 0.13 -0.8 515,947 + ybbM b0491 0.18 -0.7 515,982 + ybbM b0491 0.22 +1.0 515,987 + ybbM b0491 0.23 -2.2 515,991 + ybbM b0491 0.24 +0.2 515,991 + ybbM b0491 0.24 -0.2
Or see this region's nucleotide sequence