Strain Fitness in Escherichia coli BW25113 around b2521

Experiment: mariner vector transformation; agar assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	mariner vector transformation; agar assay
2,649,566	+	yfhM	b2520	0.85	+0.2
2,649,602	+	yfhM	b2520	0.86	-0.7
2,649,608	-	yfhM	b2520	0.86	+0.3
2,649,608	-	yfhM	b2520	0.86	+0.2
2,649,695	+	yfhM	b2520	0.88	+0.5
2,649,695	+	yfhM	b2520	0.88	+0.6
2,649,701	-	yfhM	b2520	0.88	-0.4
2,649,720	+	yfhM	b2520	0.88	+0.3
2,649,787	-	yfhM	b2520	0.89	-0.1
2,649,787	-	yfhM	b2520	0.89	+0.4
2,650,071	-				+0.1
2,650,180	+				+1.1
2,650,180	+				-0.2
2,650,281	-				-0.2
2,650,328	-				+0.5
2,650,521	-				-0.7
2,650,627	-	sseA	b2521	0.13	-1.4
2,650,627	-	sseA	b2521	0.13	+0.0
2,650,638	-	sseA	b2521	0.14	+0.1
2,650,640	+	sseA	b2521	0.15	+0.3
2,650,640	+	sseA	b2521	0.15	-1.2
2,650,711	+	sseA	b2521	0.23	-0.2
2,650,829	+	sseA	b2521	0.37	-0.2
2,650,829	+	sseA	b2521	0.37	+0.5
2,650,829	+	sseA	b2521	0.37	+0.1
2,650,829	+	sseA	b2521	0.37	-0.4
2,650,911	+	sseA	b2521	0.47	-0.1
2,650,911	+	sseA	b2521	0.47	+0.2
2,650,924	+	sseA	b2521	0.48	+0.2
2,651,060	-	sseA	b2521	0.64	+1.0
2,651,060	-	sseA	b2521	0.64	+0.2
2,651,078	+	sseA	b2521	0.66	-0.7
2,651,091	-	sseA	b2521	0.68	+0.8
2,651,091	-	sseA	b2521	0.68	-1.3
2,651,105	+	sseA	b2521	0.70	+0.1
2,651,150	+	sseA	b2521	0.75	-0.2
2,651,195	+	sseA	b2521	0.80	-0.1
2,651,195	+	sseA	b2521	0.80	-0.1
2,651,231	+	sseA	b2521	0.85	-0.5
2,651,231	+	sseA	b2521	0.85	-0.1
2,651,245	-	sseA	b2521	0.86	+0.9
2,651,248	+	sseA	b2521	0.87	-0.5
2,651,254	+	sseA	b2521	0.87	-0.4
2,651,254	+	sseA	b2521	0.87	+0.8
2,651,293	+				+0.4
2,651,294	-				-0.8
2,651,294	-				+3.5
2,651,296	+				-0.1
2,651,296	+				-0.4
2,651,306	+				-0.1
2,651,418	+				-0.9
2,651,420	-				-0.2
2,651,420	-				-0.5
2,651,430	+				-3.8
2,651,441	+				-0.1
2,651,441	+				-1.0
2,651,442	+				+0.3
2,651,442	+				-0.0
2,651,443	+				-0.5
2,651,443	+				-1.2
2,651,443	+				-1.0
2,651,443	+				-1.4
2,651,446	+				-1.3
2,651,446	+				-0.5
2,651,446	+				-1.0
2,651,451	-				+0.3
2,651,454	-				-1.5
2,651,454	-				-1.2
2,651,455	+				-1.2
2,651,455	+				+0.2
2,651,455	+				-0.9
2,651,455	+				-1.4
2,651,457	+				-0.5
2,651,461	-				-1.1
2,651,461	-				-1.0
2,651,463	-				+0.0
2,651,479	+				-1.3
2,651,479	+				-0.1
2,651,541	+				-0.6
2,651,543	+				-0.6
2,651,553	+				+0.3
2,651,575	+				-0.5
2,651,575	+				-0.2
2,651,736	+				+3.1
2,651,755	+				+1.3
2,651,756	+				-0.3
2,651,756	+				-0.6
2,651,761	-				+0.7
2,651,763	-				+0.6
2,651,767	+				-1.0
2,651,773	-				+1.3
2,651,775	-				+0.8
2,651,775	-				+0.8
2,651,775	-				+0.6
2,651,775	-				+2.4
2,651,778	-				+0.2
2,651,781	-				+0.8
2,651,789	-				-0.6
2,651,792	+				+0.3
2,651,792	+				-0.7
2,651,792	+				-0.8
2,651,792	+				-0.9
2,651,792	+				+1.3
2,651,798	-				+0.3
2,651,798	-				+1.0
2,651,798	-				+0.2
2,651,798	-				+1.0
2,651,801	+				-0.6
2,651,801	+				+0.3
2,651,804	+				+0.2
2,651,809	-				+0.2
2,651,809	-				-0.0
2,651,809	-				+0.3
2,651,809	-				+0.2
2,651,809	-				+0.6
2,651,811	+				-0.1
2,651,812	-				+0.0
2,651,820	-				+0.4
2,651,822	-				-0.0
2,651,867	+				-0.4
2,651,867	+				-0.9
2,651,867	+				-0.5
2,651,867	+				+0.5
2,651,867	+				+0.4
2,651,867	+				-0.3
2,651,867	-				+0.9
2,651,870	+				-0.1
2,651,875	-				-0.1
2,651,875	-				-0.1
2,651,875	-				-0.5
2,651,875	-				-0.2
2,651,883	-				+0.1
2,651,884	+				-0.8
2,651,884	+				+0.9
2,651,890	-				-0.2
2,651,890	-				-0.7
2,651,890	-				+0.5
2,651,908	-	ryfA	b4440	0.10	-0.3
2,651,911	+	ryfA	b4440	0.11	-0.4
2,651,911	+	ryfA	b4440	0.11	+0.0
2,651,916	+	ryfA	b4440	0.13	+0.2
2,651,918	+	ryfA	b4440	0.13	+0.2
2,651,920	-	ryfA	b4440	0.14	+0.1
2,651,920	-	ryfA	b4440	0.14	+0.1
2,651,923	+	ryfA	b4440	0.15	+0.9
2,651,923	+	ryfA	b4440	0.15	+0.4
2,651,949	+	ryfA	b4440	0.24	+1.0
2,651,949	+	ryfA	b4440	0.24	+0.5
2,651,967	-	ryfA	b4440	0.30	-0.4
2,651,984	+	ryfA	b4440	0.35	-0.4
2,651,992	+	ryfA	b4440	0.38	-1.5
2,651,995	+	ryfA	b4440	0.39	-0.3
2,652,000	-	ryfA	b4440	0.40	-1.0
2,652,003	-	ryfA	b4440	0.41	-0.1
2,652,008	+	ryfA	b4440	0.43	-0.1
2,652,008	+	ryfA	b4440	0.43	+0.0
2,652,008	+	ryfA	b4440	0.43	+0.6
2,652,008	+	ryfA	b4440	0.43	-0.2
2,652,008	+	ryfA	b4440	0.43	+0.1
2,652,008	+	ryfA	b4440	0.43	+0.4
2,652,008	+	ryfA	b4440	0.43	-1.4
2,652,016	-	ryfA	b4440	0.46	-1.2
2,652,016	-	ryfA	b4440	0.46	+0.4
2,652,018	+	ryfA	b4440	0.46	+0.1
2,652,021	-	ryfA	b4440	0.47	+0.3
2,652,027	-	ryfA	b4440	0.49	-1.7
2,652,048	+	ryfA	b4440	0.56	-0.4
2,652,048	+	ryfA	b4440	0.56	-0.0
2,652,048	+	ryfA	b4440	0.56	+0.3
2,652,068	-	ryfA	b4440	0.63	+0.5
2,652,077	-	ryfA	b4440	0.66	-0.1
2,652,092	-	ryfA	b4440	0.71	+0.1
2,652,105	+	ryfA	b4440	0.75	-0.3
2,652,108	+	ryfA	b4440	0.76	+0.2
2,652,108	+	ryfA	b4440	0.76	-0.1
2,652,111	+	ryfA	b4440	0.77	+0.0
2,652,122	+	ryfA	b4440	0.81	+0.1
2,652,122	+	ryfA	b4440	0.81	-0.3
2,652,129	+	ryfA	b4440	0.83	-0.9
2,652,129	+	ryfA	b4440	0.83	-0.5
2,652,129	+	ryfA	b4440	0.83	+1.4
2,652,129	+	ryfA	b4440	0.83	-0.4
2,652,136	+	ryfA	b4440	0.85	+0.6
2,652,136	+	ryfA	b4440	0.85	-0.1
2,652,136	+	ryfA	b4440	0.85	-0.7
2,652,137	-	ryfA	b4440	0.86	+1.1
2,652,138	+	ryfA	b4440	0.86	-0.0
2,652,139	+	ryfA	b4440	0.86	+0.5
2,652,139	+	ryfA	b4440	0.86	-0.3
2,652,139	+	ryfA	b4440	0.86	+0.1
2,652,139	+	ryfA	b4440	0.86	-0.2
2,652,139	+	ryfA	b4440	0.86	+0.8
2,652,139	+	ryfA	b4440	0.86	-0.0
2,652,139	+	ryfA	b4440	0.86	-0.9
2,652,143	+	ryfA	b4440	0.88	-0.9
2,652,143	+	ryfA	b4440	0.88	+0.4
2,652,143	+	ryfA	b4440	0.88	+0.2
2,652,143	+	ryfA	b4440	0.88	-0.4
2,652,147	+	ryfA	b4440	0.89	-0.5
2,652,147	+	ryfA	b4440	0.89	-1.4
2,652,148	+	ryfA	b4440	0.89	+0.1
2,652,148	+	ryfA	b4440	0.89	-0.2
2,652,149	-	ryfA	b4440	0.89	-0.4
2,652,151	-				+0.5
2,652,156	-				-1.2
2,652,219	+				-0.8
2,652,219	+				-0.1
2,652,227	-				-0.6
2,652,227	-				-0.2
2,652,229	+				+1.5
2,652,239	-				+0.5
2,652,239	-				+0.2
2,652,251	-				+1.1
2,652,251	-				+0.9
2,652,255	+				-1.3
2,652,303	-	sseB	b2522	0.16	-0.8
2,652,303	-	sseB	b2522	0.16	-0.3
2,652,310	+	sseB	b2522	0.17	+0.3
2,652,318	-				+0.8
2,652,318	-	sseB	b2522	0.18	+0.6
2,652,318	-	sseB	b2522	0.18	+1.5
2,652,318	-	sseB	b2522	0.18	-0.8
2,652,318	-	sseB	b2522	0.18	+0.6
2,652,318	-	sseB	b2522	0.18	-0.7
2,652,322	+	sseB	b2522	0.18	+2.5
2,652,325	-	sseB	b2522	0.19	+0.9
2,652,326	-	sseB	b2522	0.19	+0.5
2,652,330	-	sseB	b2522	0.19	+0.2
2,652,330	-	sseB	b2522	0.19	-0.1
2,652,342	-	sseB	b2522	0.21	+0.2
2,652,342	-	sseB	b2522	0.21	+0.9
2,652,345	-	sseB	b2522	0.21	-0.4
2,652,345	-	sseB	b2522	0.21	+0.3