Strain Fitness in Escherichia coli BW25113 around b4154

Experiment: vcDART vector transformation; agar assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; agar assay
4,377,550	+	frdC	b4152	0.38	-0.7
4,377,576	+	frdC	b4152	0.44	+0.3
4,377,777	+				-0.1
4,377,794	+				+0.1
4,377,794	+				+0.2
4,377,812	-				-0.0
4,377,812	-				+0.7
4,377,812	-				-1.3
4,377,889	+	frdB	b4153	0.11	-0.1
4,377,889	-	frdB	b4153	0.11	+0.5
4,377,909	-	frdB	b4153	0.14	-0.1
4,377,909	-	frdB	b4153	0.14	-2.5
4,377,919	+	frdB	b4153	0.15	-0.3
4,377,939	-	frdB	b4153	0.18	-0.9
4,377,966	-	frdB	b4153	0.22	+2.0
4,377,972	+	frdB	b4153	0.23	+0.4
4,377,976	+	frdB	b4153	0.23	+0.4
4,377,980	-	frdB	b4153	0.24	-1.1
4,377,990	-	frdB	b4153	0.25	-0.3
4,378,039	+	frdB	b4153	0.32	-0.6
4,378,039	+	frdB	b4153	0.32	-0.4
4,378,079	-	frdB	b4153	0.37	+0.0
4,378,079	-	frdB	b4153	0.37	-0.1
4,378,106	-	frdB	b4153	0.41	-1.0
4,378,134	+	frdB	b4153	0.45	+0.2
4,378,134	+	frdB	b4153	0.45	-0.3
4,378,161	+	frdB	b4153	0.48	-0.2
4,378,261	-	frdB	b4153	0.62	-0.1
4,378,265	-	frdB	b4153	0.62	-0.1
4,378,272	+	frdB	b4153	0.63	+1.4
4,378,272	+	frdB	b4153	0.63	-0.8
4,378,301	+	frdB	b4153	0.67	+0.6
4,378,393	+	frdB	b4153	0.80	-1.0
4,378,403	+	frdB	b4153	0.81	+0.3
4,378,427	-	frdB	b4153	0.84	-0.3
4,378,453	-	frdB	b4153	0.88	+1.2
4,378,454	-	frdB	b4153	0.88	+0.0
4,378,475	-				+1.4
4,378,475	-				+0.4
4,378,646	+				-0.3
4,378,646	+				-0.4
4,378,665	-				+0.9
4,378,699	-				+0.1
4,378,699	-				-1.5
4,378,720	+	frdA	b4154	0.10	+0.0
4,378,726	+	frdA	b4154	0.11	-0.0
4,378,726	+	frdA	b4154	0.11	-0.5
4,378,740	+	frdA	b4154	0.11	+1.3
4,378,758	-	frdA	b4154	0.12	+1.0
4,378,768	-	frdA	b4154	0.13	-0.1
4,378,768	-	frdA	b4154	0.13	+0.1
4,378,777	-	frdA	b4154	0.13	+0.6
4,378,777	-	frdA	b4154	0.13	+0.4
4,378,789	-	frdA	b4154	0.14	+0.4
4,378,789	-	frdA	b4154	0.14	+1.0
4,378,800	+	frdA	b4154	0.15	-0.9
4,378,800	+	frdA	b4154	0.15	+0.1
4,378,800	+	frdA	b4154	0.15	-0.6
4,378,800	+	frdA	b4154	0.15	-0.2
4,378,809	-	frdA	b4154	0.15	+0.0
4,378,825	+	frdA	b4154	0.16	-0.4
4,378,825	+	frdA	b4154	0.16	-0.7
4,378,825	-	frdA	b4154	0.16	+0.1
4,378,825	-	frdA	b4154	0.16	-0.7
4,378,825	-	frdA	b4154	0.16	-0.6
4,378,833	-	frdA	b4154	0.17	-0.1
4,378,840	+	frdA	b4154	0.17	-0.9
4,378,850	+	frdA	b4154	0.18	-0.0
4,378,858	-	frdA	b4154	0.18	-0.0
4,378,903	-	frdA	b4154	0.20	+0.0
4,378,903	-	frdA	b4154	0.20	+0.4
4,378,906	-	frdA	b4154	0.21	+0.3
4,378,935	-	frdA	b4154	0.22	-0.2
4,378,935	-	frdA	b4154	0.22	+0.5
4,378,956	-	frdA	b4154	0.23	+0.2
4,379,115	-	frdA	b4154	0.32	+0.9
4,379,179	-	frdA	b4154	0.36	-0.1
4,379,181	+	frdA	b4154	0.36	-0.9
4,379,181	+	frdA	b4154	0.36	+0.1
4,379,186	+	frdA	b4154	0.36	+0.1
4,379,186	+	frdA	b4154	0.36	+1.5
4,379,199	+	frdA	b4154	0.37	-1.4
4,379,199	-	frdA	b4154	0.37	+0.8
4,379,208	-	frdA	b4154	0.37	+0.1
4,379,271	-	frdA	b4154	0.41	-0.4
4,379,271	-	frdA	b4154	0.41	+1.2
4,379,271	-	frdA	b4154	0.41	+0.0
4,379,279	-	frdA	b4154	0.41	-0.7
4,379,285	+	frdA	b4154	0.42	+1.4
4,379,289	+	frdA	b4154	0.42	-0.7
4,379,354	-	frdA	b4154	0.45	-0.5
4,379,410	+	frdA	b4154	0.48	+0.6
4,379,438	-	frdA	b4154	0.50	+0.6
4,379,445	-	frdA	b4154	0.50	-0.7
4,379,445	-	frdA	b4154	0.50	-1.4
4,379,526	+	frdA	b4154	0.55	-0.2
4,379,646	+	frdA	b4154	0.62	-1.3
4,379,646	+	frdA	b4154	0.62	-1.7
4,379,672	+	frdA	b4154	0.63	+0.3
4,379,672	+	frdA	b4154	0.63	+0.0
4,379,698	-	frdA	b4154	0.64	-0.0
4,379,698	-	frdA	b4154	0.64	-0.3
4,379,719	+	frdA	b4154	0.66	-0.5
4,379,719	+	frdA	b4154	0.66	+0.1
4,379,803	-	frdA	b4154	0.70	+0.2
4,379,822	+	frdA	b4154	0.71	-0.0
4,379,824	-	frdA	b4154	0.71	-0.8
4,379,824	-	frdA	b4154	0.71	+0.6
4,379,858	+	frdA	b4154	0.73	+0.1
4,379,941	-	frdA	b4154	0.78	+0.3
4,379,970	-	frdA	b4154	0.79	-0.7
4,380,036	-	frdA	b4154	0.83	+0.2
4,380,055	-	frdA	b4154	0.84	+0.6
4,380,072	+	frdA	b4154	0.85	+0.2
4,380,083	-	frdA	b4154	0.86	-0.2
4,380,102	-	frdA	b4154	0.87	+0.7
4,380,124	+	frdA	b4154	0.88	-0.1
4,380,159	+	frdA	b4154	0.90	-0.8
4,380,159	+	frdA	b4154	0.90	-0.6
4,380,159	+	frdA	b4154	0.90	-0.8
4,380,165	+				-0.4
4,380,165	+				-1.0
4,380,197	-				-0.1
4,380,205	+				+0.2
4,380,211	-				-0.2
4,380,306	-				+0.2
4,380,380	+				-0.3
4,380,440	+				+0.7
4,380,441	-				-0.4
4,380,443	+				+0.3
4,380,448	+				+0.3
4,380,451	-				+1.0
4,380,451	-				-0.4
4,380,451	-				+0.3
4,380,581	-				+0.4
4,380,581	-				+0.4