Experiment: vcDART vector transformation; agar assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysS and ybcI are separated by 35 nucleotides ybcI and ybcJ are separated by 107 nucleotides ybcJ and folD are separated by 1 nucleotides folD and sfmA are separated by 470 nucleotides
b0526: cysS - cysteinyl-tRNA synthetase (NCBI), at 553,834 to 555,219
cysS
b0527: ybcI - conserved inner membrane protein (NCBI), at 555,255 to 555,776
ybcI
b0528: ybcJ - orf, hypothetical protein (VIMSS), at 555,884 to 556,096
ybcJ
b0529: folD - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase (NCBI), at 556,098 to 556,964
folD
b0530: sfmA - putative fimbrial-like protein (VIMSS), at 557,435 to 557,977
sfmA
Position (kb) 556
557 Strain fitness (log2 ratio) -1
0
1
2
3 at 555.277 kb on + strand at 555.277 kb on + strand at 555.306 kb on + strand at 555.340 kb on - strand, within ybcI at 555.425 kb on + strand, within ybcI at 555.455 kb on + strand, within ybcI at 555.553 kb on + strand, within ybcI at 555.629 kb on - strand, within ybcI at 555.663 kb on + strand, within ybcI at 555.761 kb on + strand at 555.815 kb on + strand at 555.823 kb on - strand at 555.895 kb on + strand at 555.898 kb on + strand at 555.902 kb on + strand at 557.005 kb on + strand at 557.074 kb on + strand at 557.074 kb on + strand at 557.081 kb on + strand at 557.086 kb on + strand at 557.090 kb on + strand at 557.092 kb on - strand at 557.092 kb on - strand at 557.094 kb on + strand at 557.125 kb on + strand at 557.125 kb on + strand at 557.171 kb on + strand at 557.226 kb on + strand at 557.584 kb on + strand, within sfmA at 557.588 kb on + strand, within sfmA at 557.707 kb on + strand, within sfmA at 557.767 kb on + strand, within sfmA at 557.786 kb on + strand, within sfmA at 557.824 kb on + strand, within sfmA at 557.838 kb on + strand, within sfmA at 557.845 kb on + strand, within sfmA at 557.856 kb on + strand, within sfmA at 557.870 kb on + strand, within sfmA at 557.900 kb on + strand, within sfmA at 557.900 kb on + strand, within sfmA at 557.902 kb on + strand, within sfmA at 557.902 kb on + strand, within sfmA at 557.914 kb on + strand, within sfmA at 557.916 kb on + strand, within sfmA at 557.916 kb on + strand, within sfmA at 557.925 kb on + strand at 557.925 kb on + strand at 557.928 kb on + strand at 557.933 kb on + strand at 557.938 kb on + strand at 557.940 kb on + strand at 557.957 kb on + strand at 557.957 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; agar assay remove 555,277 + +0.5 555,277 + +1.1 555,306 + -0.9 555,340 - ybcI b0527 0.16 -0.0 555,425 + ybcI b0527 0.33 +0.7 555,455 + ybcI b0527 0.38 +0.3 555,553 + ybcI b0527 0.57 -0.4 555,629 - ybcI b0527 0.72 -0.4 555,663 + ybcI b0527 0.78 +0.0 555,761 + +0.4 555,815 + +1.0 555,823 - -0.0 555,895 + -0.9 555,898 + +2.8 555,902 + -0.4 557,005 + +0.1 557,074 + +1.1 557,074 + -0.1 557,081 + -1.1 557,086 + -0.0 557,090 + -0.3 557,092 - +1.8 557,092 - +1.8 557,094 + -0.8 557,125 + -0.4 557,125 + -0.7 557,171 + -0.7 557,226 + -0.3 557,584 + sfmA b0530 0.27 +0.5 557,588 + sfmA b0530 0.28 +3.4 557,707 + sfmA b0530 0.50 +1.8 557,767 + sfmA b0530 0.61 +1.8 557,786 + sfmA b0530 0.65 -0.2 557,824 + sfmA b0530 0.72 +1.8 557,838 + sfmA b0530 0.74 +2.8 557,845 + sfmA b0530 0.76 +0.2 557,856 + sfmA b0530 0.78 -0.8 557,870 + sfmA b0530 0.80 -0.2 557,900 + sfmA b0530 0.86 -1.5 557,900 + sfmA b0530 0.86 -1.0 557,902 + sfmA b0530 0.86 -0.5 557,902 + sfmA b0530 0.86 -1.0 557,914 + sfmA b0530 0.88 -1.6 557,916 + sfmA b0530 0.89 +0.2 557,916 + sfmA b0530 0.89 -1.0 557,925 + +1.0 557,925 + -0.4 557,928 + -1.5 557,933 + -0.2 557,938 + -0.8 557,940 + -0.8 557,957 + -0.5 557,957 + -0.9
Or see this region's nucleotide sequence