Experiment: vcDART vector transformation; agar assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panE and yajQ are separated by 167 nucleotides yajQ and yajR are separated by 127 nucleotides
b0425: panE - 2-dehydropantoate 2-reductase (NCBI), at 442,828 to 443,739
panE
b0426: yajQ - orf, hypothetical protein (VIMSS), at 443,907 to 444,398
yajQ
b0427: yajR - putative transport protein (VIMSS), at 444,526 to 445,890
yajR
Position (kb) 443
444
445 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 442.917 kb on - strand at 443.001 kb on - strand, within panE at 443.091 kb on - strand, within panE at 443.139 kb on + strand, within panE at 443.147 kb on - strand, within panE at 443.147 kb on - strand, within panE at 443.261 kb on - strand, within panE at 443.261 kb on - strand, within panE at 443.298 kb on - strand, within panE at 443.304 kb on + strand, within panE at 443.336 kb on - strand, within panE at 443.398 kb on + strand, within panE at 443.398 kb on + strand, within panE at 443.409 kb on + strand, within panE at 443.468 kb on - strand, within panE at 443.471 kb on + strand, within panE at 443.471 kb on + strand, within panE at 443.471 kb on + strand, within panE at 443.479 kb on - strand, within panE at 443.481 kb on + strand, within panE at 443.481 kb on + strand, within panE at 443.601 kb on + strand, within panE at 443.617 kb on - strand, within panE at 443.627 kb on - strand, within panE at 443.637 kb on - strand, within panE at 443.713 kb on + strand at 443.713 kb on + strand at 443.779 kb on + strand at 443.786 kb on + strand at 443.792 kb on - strand at 443.792 kb on - strand at 443.794 kb on - strand at 443.794 kb on - strand at 443.860 kb on - strand at 443.870 kb on + strand at 444.002 kb on + strand, within yajQ at 444.009 kb on - strand, within yajQ at 444.062 kb on - strand, within yajQ at 444.062 kb on - strand, within yajQ at 444.099 kb on - strand, within yajQ at 444.147 kb on + strand, within yajQ at 444.206 kb on + strand, within yajQ at 444.206 kb on + strand, within yajQ at 444.209 kb on + strand, within yajQ at 444.331 kb on + strand, within yajQ at 444.333 kb on + strand, within yajQ at 444.342 kb on + strand at 444.342 kb on + strand, within yajQ at 444.487 kb on + strand at 444.498 kb on + strand at 444.629 kb on - strand at 444.641 kb on - strand at 444.666 kb on + strand, within yajR at 444.666 kb on + strand, within yajR at 444.715 kb on - strand, within yajR at 444.757 kb on + strand, within yajR at 444.757 kb on + strand, within yajR at 444.791 kb on - strand, within yajR at 444.877 kb on - strand, within yajR at 444.877 kb on - strand, within yajR at 444.877 kb on - strand, within yajR at 444.882 kb on - strand, within yajR at 444.882 kb on - strand, within yajR at 444.883 kb on + strand, within yajR at 444.908 kb on - strand, within yajR at 444.976 kb on - strand, within yajR at 444.986 kb on - strand, within yajR at 444.986 kb on - strand, within yajR at 445.022 kb on + strand, within yajR at 445.032 kb on + strand, within yajR at 445.082 kb on - strand, within yajR at 445.114 kb on + strand, within yajR at 445.114 kb on + strand, within yajR at 445.138 kb on + strand, within yajR at 445.138 kb on + strand, within yajR at 445.147 kb on - strand, within yajR at 445.166 kb on + strand, within yajR at 445.300 kb on - strand, within yajR at 445.306 kb on + strand, within yajR at 445.319 kb on + strand, within yajR at 445.319 kb on + strand, within yajR at 445.355 kb on + strand, within yajR at 445.376 kb on - strand, within yajR at 445.376 kb on - strand, within yajR at 445.382 kb on + strand, within yajR
Per-strain Table
Position Strand Gene LocusTag Fraction vcDART vector transformation; agar assay remove 442,917 - -0.2 443,001 - panE b0425 0.19 -0.1 443,091 - panE b0425 0.29 +0.4 443,139 + panE b0425 0.34 +0.4 443,147 - panE b0425 0.35 +1.1 443,147 - panE b0425 0.35 +0.4 443,261 - panE b0425 0.47 -1.3 443,261 - panE b0425 0.47 -0.4 443,298 - panE b0425 0.52 -0.0 443,304 + panE b0425 0.52 -0.0 443,336 - panE b0425 0.56 -0.6 443,398 + panE b0425 0.62 +0.1 443,398 + panE b0425 0.62 -0.5 443,409 + panE b0425 0.64 -0.3 443,468 - panE b0425 0.70 -0.2 443,471 + panE b0425 0.71 -0.8 443,471 + panE b0425 0.71 +1.7 443,471 + panE b0425 0.71 -1.2 443,479 - panE b0425 0.71 +0.4 443,481 + panE b0425 0.72 +0.1 443,481 + panE b0425 0.72 +0.0 443,601 + panE b0425 0.85 -0.8 443,617 - panE b0425 0.87 +0.2 443,627 - panE b0425 0.88 -1.0 443,637 - panE b0425 0.89 -0.2 443,713 + -1.3 443,713 + +0.2 443,779 + -1.2 443,786 + -1.3 443,792 - -0.9 443,792 - -0.9 443,794 - +0.6 443,794 - -0.5 443,860 - -1.1 443,870 + -0.1 444,002 + yajQ b0426 0.19 -0.1 444,009 - yajQ b0426 0.21 +0.2 444,062 - yajQ b0426 0.32 +0.7 444,062 - yajQ b0426 0.32 +0.1 444,099 - yajQ b0426 0.39 -0.1 444,147 + yajQ b0426 0.49 +0.0 444,206 + yajQ b0426 0.61 +0.3 444,206 + yajQ b0426 0.61 -0.5 444,209 + yajQ b0426 0.61 +0.0 444,331 + yajQ b0426 0.86 -0.2 444,333 + yajQ b0426 0.87 -0.3 444,342 + -0.1 444,342 + yajQ b0426 0.88 +0.3 444,487 + -0.4 444,498 + +0.3 444,629 - +2.7 444,641 - +2.6 444,666 + yajR b0427 0.10 +3.4 444,666 + yajR b0427 0.10 +1.9 444,715 - yajR b0427 0.14 +2.9 444,757 + yajR b0427 0.17 +2.2 444,757 + yajR b0427 0.17 +3.2 444,791 - yajR b0427 0.19 +2.9 444,877 - yajR b0427 0.26 +4.8 444,877 - yajR b0427 0.26 +3.3 444,877 - yajR b0427 0.26 +3.3 444,882 - yajR b0427 0.26 +3.7 444,882 - yajR b0427 0.26 +1.8 444,883 + yajR b0427 0.26 +2.1 444,908 - yajR b0427 0.28 +2.1 444,976 - yajR b0427 0.33 +3.9 444,986 - yajR b0427 0.34 +2.7 444,986 - yajR b0427 0.34 +3.1 445,022 + yajR b0427 0.36 +3.6 445,032 + yajR b0427 0.37 +2.9 445,082 - yajR b0427 0.41 +2.8 445,114 + yajR b0427 0.43 +2.4 445,114 + yajR b0427 0.43 +3.0 445,138 + yajR b0427 0.45 +1.9 445,138 + yajR b0427 0.45 +2.8 445,147 - yajR b0427 0.45 +2.2 445,166 + yajR b0427 0.47 +2.8 445,300 - yajR b0427 0.57 +3.0 445,306 + yajR b0427 0.57 +2.8 445,319 + yajR b0427 0.58 +3.0 445,319 + yajR b0427 0.58 +4.0 445,355 + yajR b0427 0.61 +3.2 445,376 - yajR b0427 0.62 +2.4 445,376 - yajR b0427 0.62 +2.8 445,382 + yajR b0427 0.63 +3.3
Or see this region's nucleotide sequence