Strain Fitness in Escherichia coli BW25113 around b4268

Experiment: vcDART vector transformation; agar assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; agar assay
4,491,663	-	idnD	b4267	0.26	-0.9
4,491,663	-	idnD	b4267	0.26	-0.5
4,491,668	+	idnD	b4267	0.26	-0.5
4,491,675	-	idnD	b4267	0.27	-0.1
4,491,675	-	idnD	b4267	0.27	-0.6
4,491,687	+	idnD	b4267	0.28	-0.7
4,491,687	+	idnD	b4267	0.28	+0.7
4,491,695	-	idnD	b4267	0.29	+0.0
4,491,695	-	idnD	b4267	0.29	-0.2
4,491,696	+	idnD	b4267	0.29	+1.2
4,491,696	+	idnD	b4267	0.29	+0.7
4,491,699	-	idnD	b4267	0.29	-0.6
4,491,714	-	idnD	b4267	0.31	+0.4
4,491,778	-	idnD	b4267	0.37	-0.0
4,491,789	-	idnD	b4267	0.38	-1.8
4,491,793	+	idnD	b4267	0.38	-0.9
4,491,793	+	idnD	b4267	0.38	+1.1
4,491,826	+	idnD	b4267	0.41	-0.6
4,491,826	+	idnD	b4267	0.41	+0.4
4,491,846	-	idnD	b4267	0.43	-0.8
4,491,846	-	idnD	b4267	0.43	-0.8
4,491,875	-	idnD	b4267	0.46	-1.0
4,491,892	+	idnD	b4267	0.48	-0.6
4,491,900	-	idnD	b4267	0.49	-1.1
4,491,900	-	idnD	b4267	0.49	-0.3
4,491,900	-	idnD	b4267	0.49	-0.1
4,491,931	-	idnD	b4267	0.52	-0.7
4,491,931	-	idnD	b4267	0.52	-0.2
4,491,943	-	idnD	b4267	0.53	-0.6
4,491,944	-	idnD	b4267	0.53	-2.7
4,492,012	+	idnD	b4267	0.59	+0.6
4,492,016	-	idnD	b4267	0.60	-0.9
4,492,016	-	idnD	b4267	0.60	+0.6
4,492,021	-	idnD	b4267	0.60	-1.3
4,492,039	+	idnD	b4267	0.62	-0.6
4,492,104	+	idnD	b4267	0.68	+0.4
4,492,104	+	idnD	b4267	0.68	-1.1
4,492,104	+	idnD	b4267	0.68	-1.4
4,492,104	+	idnD	b4267	0.68	-0.2
4,492,104	+	idnD	b4267	0.68	+0.6
4,492,104	+	idnD	b4267	0.68	-1.2
4,492,104	+	idnD	b4267	0.68	+0.4
4,492,104	+	idnD	b4267	0.68	-0.5
4,492,104	+	idnD	b4267	0.68	-0.2
4,492,104	+	idnD	b4267	0.68	-1.1
4,492,104	+	idnD	b4267	0.68	-0.5
4,492,107	+	idnD	b4267	0.69	-0.5
4,492,114	+	idnD	b4267	0.69	-0.7
4,492,126	+	idnD	b4267	0.71	-0.5
4,492,136	+	idnD	b4267	0.72	+0.2
4,492,136	+	idnD	b4267	0.72	-1.4
4,492,137	+	idnD	b4267	0.72	-0.5
4,492,160	+	idnD	b4267	0.74	-1.4
4,492,205	+	idnD	b4267	0.78	-0.9
4,492,238	+	idnD	b4267	0.81	+2.6
4,492,239	-	idnD	b4267	0.81	+1.6
4,492,339	-				+0.0
4,492,367	-				-2.1
4,492,367	-				+0.5
4,492,407	-				+1.6
4,492,407	-				+0.6
4,492,409	+				+1.6
4,492,412	-				-0.0
4,492,445	-				-1.0
4,492,448	-				-0.2
4,492,449	-				-1.8
4,492,449	-				-0.0
4,492,450	-				-2.4
4,492,450	-				-0.3
4,492,451	-				+0.3
4,492,453	-				-1.1
4,492,455	-				-0.7
4,492,455	-				-2.3
4,492,463	-				+1.6
4,492,529	+				-0.5
4,492,610	+				-1.2
4,492,813	-	idnK	b4268	0.30	+0.1
4,492,824	+	idnK	b4268	0.32	+0.1
4,492,922	-	idnK	b4268	0.49	-0.5
4,492,922	-	idnK	b4268	0.49	-1.8
4,492,927	-	idnK	b4268	0.50	-1.3
4,492,939	-	idnK	b4268	0.52	-0.0
4,492,944	-	idnK	b4268	0.53	-1.1
4,492,951	-	idnK	b4268	0.54	-1.4
4,492,951	-	idnK	b4268	0.54	-0.6
4,492,951	-	idnK	b4268	0.54	-0.1
4,492,951	-	idnK	b4268	0.54	-0.3
4,492,951	-	idnK	b4268	0.54	-1.0
4,492,965	-	idnK	b4268	0.57	-1.1
4,492,965	-	idnK	b4268	0.57	-1.2
4,492,994	-	idnK	b4268	0.62	-0.4
4,493,034	-	idnK	b4268	0.69	-0.3
4,493,058	-	idnK	b4268	0.73	-1.8
4,493,061	-	idnK	b4268	0.74	-0.1
4,493,067	-	idnK	b4268	0.75	-1.1
4,493,067	-	idnK	b4268	0.75	-0.4
4,493,067	-	idnK	b4268	0.75	-0.4
4,493,069	+	idnK	b4268	0.75	-0.5
4,493,076	+	idnK	b4268	0.76	-0.1
4,493,076	+	idnK	b4268	0.76	+0.1
4,493,145	-	idnK	b4268	0.88	-1.6
4,493,145	-	idnK	b4268	0.88	-0.1
4,493,149	-	idnK	b4268	0.89	-0.3
4,493,166	-				-0.9
4,493,166	-				-0.4
4,493,169	-				+0.4
4,493,192	+				-1.5
4,493,195	-				-2.0
4,493,200	-				-0.0
4,493,238	-				-2.1
4,493,248	-				-0.3
4,493,248	-				+1.0
4,493,314	+				-0.1
4,493,327	-	yjgB	b4269	0.11	+0.1
4,493,327	-	yjgB	b4269	0.11	-0.4
4,493,346	+	yjgB	b4269	0.13	-0.3
4,493,346	+	yjgB	b4269	0.13	-0.3
4,493,383	-	yjgB	b4269	0.17	-0.4
4,493,383	-	yjgB	b4269	0.17	-1.2
4,493,674	+	yjgB	b4269	0.45	+0.4
4,493,682	-	yjgB	b4269	0.46	-0.5
4,493,731	-	yjgB	b4269	0.51	-0.5
4,493,838	-	yjgB	b4269	0.61	-0.4
4,493,844	+	yjgB	b4269	0.62	-0.2
4,493,946	+	yjgB	b4269	0.72	-0.2
4,493,946	+	yjgB	b4269	0.72	+0.2
4,493,949	-	yjgB	b4269	0.72	-1.0
4,494,002	-	yjgB	b4269	0.77	+0.6