Strain Fitness in Escherichia coli BW25113 around b0281

Experiment: vcDART vector transformation; agar assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; agar assay
293,928	+	yagM	b0279	0.89	+3.2
293,928	+	yagM	b0279	0.89	+1.7
293,933	+	yagM	b0279	0.89	+1.7
293,936	+	yagM	b0279	0.90	+2.7
293,937	-	yagM	b0279	0.90	+1.7
293,938	+				+0.1
293,938	+				+0.1
293,938	+				+1.7
293,960	-				+0.7
294,107	-				-0.6
294,107	-				-0.7
294,195	-				-1.6
294,200	-				+0.0
294,200	-				+0.7
294,206	-				-1.2
294,206	-				+0.1
294,225	-				-0.2
294,232	+				+1.7
294,240	-				-0.4
294,240	-				+1.1
294,240	-				+0.0
294,240	-				+0.1
294,275	-				+0.0
294,330	-				-2.2
294,338	-				+0.3
294,387	-				+0.1
294,387	-				+2.9
294,389	-				+1.1
294,389	-				-0.5
294,423	+	yagN	b0280	0.14	+0.1
294,453	-	yagN	b0280	0.20	-1.5
294,453	-	yagN	b0280	0.20	-0.5
294,466	+	yagN	b0280	0.23	-1.0
294,470	-	yagN	b0280	0.24	-0.2
294,480	-	yagN	b0280	0.27	-1.5
294,488	-	yagN	b0280	0.28	+0.1
294,488	-	yagN	b0280	0.28	+0.4
294,488	-	yagN	b0280	0.28	+0.1
294,490	+	yagN	b0280	0.29	+0.3
294,491	+	yagN	b0280	0.29	-0.8
294,523	+	yagN	b0280	0.36	-0.6
294,523	+	yagN	b0280	0.36	-0.2
294,523	+	yagN	b0280	0.36	-1.6
294,531	+	yagN	b0280	0.38	+0.5
294,539	-	yagN	b0280	0.40	-0.7
294,663	-	yagN	b0280	0.68	+0.2
294,667	+	yagN	b0280	0.69	-0.3
294,686	-	yagN	b0280	0.73	-0.3
294,698	+	yagN	b0280	0.76	-1.1
294,761	-				+0.8
294,761	-				+0.1
294,777	+				-1.3
294,809	+				-0.2
294,822	-				-0.4
294,847	+				-1.5
294,855	-				-1.0
295,050	+				-1.1
295,050	+				-0.8
295,175	+	intF	b0281	0.18	-0.3
295,183	-	intF	b0281	0.19	+0.1
295,183	-	intF	b0281	0.19	-0.2
295,238	-	intF	b0281	0.23	-1.1
295,399	-	intF	b0281	0.34	-0.9
295,399	-	intF	b0281	0.34	-0.4
295,470	+	intF	b0281	0.39	-0.5
295,470	+	intF	b0281	0.39	-1.8
295,508	+	intF	b0281	0.42	-0.4
295,567	+	intF	b0281	0.46	-1.1
295,579	-	intF	b0281	0.47	-0.6
295,579	-	intF	b0281	0.47	+0.3
295,626	+	intF	b0281	0.50	-0.5
295,648	-	intF	b0281	0.52	-1.0
295,648	-	intF	b0281	0.52	-0.7
295,650	+	intF	b0281	0.52	-0.7
295,654	+	intF	b0281	0.52	-0.4
295,680	-	intF	b0281	0.54	-0.1
295,692	+	intF	b0281	0.55	+0.2
295,698	-	intF	b0281	0.56	-0.5
295,699	+	intF	b0281	0.56	-1.0
295,700	-	intF	b0281	0.56	-0.7
295,700	-	intF	b0281	0.56	-0.8
295,724	-	intF	b0281	0.57	-2.0
295,724	-	intF	b0281	0.57	+1.4
295,796	+	intF	b0281	0.63	-1.7
295,826	-	intF	b0281	0.65	+0.6
295,826	-	intF	b0281	0.65	+0.4
295,974	-	intF	b0281	0.75	-0.3
296,002	+	intF	b0281	0.77	-0.4
296,012	+	intF	b0281	0.78	+0.1
296,012	+	intF	b0281	0.78	-1.0
296,027	+	intF	b0281	0.79	-0.1
296,059	+	intF	b0281	0.81	-0.7
296,059	+	intF	b0281	0.81	-0.6
296,202	-				+0.2
296,204	-				+0.1
296,204	-				-0.4
296,456	-	ptwF	b4629	0.53	-1.3
296,543	+				+0.6
296,543	+				-1.3
296,551	-				+0.1
296,635	-				-1.2
296,635	-				-2.6
296,655	+	yagP	b4694	0.15	-0.5
296,813	+	yagP	b4694	0.61	-0.1
296,813	+	yagP	b4694	0.61	+0.5
296,818	-	yagP	b4694	0.63	+0.3
296,818	-	yagP	b4694	0.63	+0.2
296,896	+	yagP	b4694	0.86	-0.0
296,930	+				-0.4
296,991	+	ykgT	b4695	0.68	+0.6
296,993	-	ykgT	b4695	0.71	-0.2
296,993	-	ykgT	b4695	0.71	+0.2
297,046	-				+0.1
297,064	+				-0.1
297,064	+				+0.5
297,068	+				+0.0
297,068	+				+0.4
297,082	-				-0.8
297,111	-	yagQ	b0283	0.12	-1.2
297,111	-	yagQ	b0283	0.12	-0.5
297,144	-	yagQ	b0283	0.16	-0.4
297,144	-	yagQ	b0283	0.16	-0.6
297,185	+	yagQ	b0283	0.20	-0.4
297,185	+	yagQ	b0283	0.20	+0.1
297,193	-	yagQ	b0283	0.21	-1.8
297,226	-	yagQ	b0283	0.24	-0.1
297,226	-	yagQ	b0283	0.24	+0.5
297,316	-	yagQ	b0283	0.34	+0.5
297,316	-	yagQ	b0283	0.34	-0.5