Strain Fitness in Escherichia coli BW25113 around b3329

Experiment: vcDART vector transformation; agar assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; agar assay
3,458,505	-	gspF	b3327	0.56	+0.2
3,458,521	-	gspF	b3327	0.57	+1.1
3,458,521	-	gspF	b3327	0.57	-0.2
3,458,632	-	gspF	b3327	0.66	+0.1
3,458,632	-	gspF	b3327	0.66	+0.0
3,458,638	+	gspF	b3327	0.67	+0.5
3,458,663	-	gspF	b3327	0.69	+0.5
3,458,668	+	gspF	b3327	0.69	+0.5
3,458,671	-	gspF	b3327	0.70	-0.0
3,458,687	-	gspF	b3327	0.71	-0.1
3,458,687	-	gspF	b3327	0.71	+1.4
3,458,689	+	gspF	b3327	0.71	-0.4
3,458,711	+	gspF	b3327	0.73	-1.2
3,458,817	+	gspF	b3327	0.82	-0.6
3,458,817	+	gspF	b3327	0.82	+0.2
3,458,822	+	gspF	b3327	0.82	+0.9
3,458,822	+	gspF	b3327	0.82	-0.7
3,458,823	+	gspF	b3327	0.82	+0.5
3,458,878	+	gspF	b3327	0.87	+1.1
3,458,889	+	gspF	b3327	0.88	-0.7
3,458,899	-	gspF	b3327	0.89	-0.4
3,458,909	-	gspF	b3327	0.89	+0.1
3,458,913	+	gspF	b3327	0.90	-0.1
3,458,920	+				+0.4
3,458,928	-				-0.2
3,458,936	+				-0.7
3,458,938	-				+0.2
3,458,949	-				-1.3
3,458,978	+				+0.1
3,458,980	+				-0.9
3,458,986	-				-0.9
3,459,001	+				-0.2
3,459,004	-				+0.1
3,459,015	-				-0.3
3,459,027	-				+0.7
3,459,078	-				-0.5
3,459,078	-				+0.8
3,459,203	-	gspG	b3328	0.36	+0.6
3,459,203	-	gspG	b3328	0.36	-0.8
3,459,226	-	gspG	b3328	0.41	-0.4
3,459,244	-	gspG	b3328	0.45	-0.4
3,459,252	+	gspG	b3328	0.47	+0.8
3,459,252	+	gspG	b3328	0.47	-0.0
3,459,288	-	gspG	b3328	0.55	+0.6
3,459,288	-	gspG	b3328	0.55	-0.2
3,459,320	+	gspG	b3328	0.63	-1.2
3,459,320	+	gspG	b3328	0.63	-0.0
3,459,324	+	gspG	b3328	0.64	+0.6
3,459,355	-	gspG	b3328	0.71	+1.6
3,459,360	+	gspG	b3328	0.72	+0.3
3,459,360	+	gspG	b3328	0.72	-1.4
3,459,360	+	gspG	b3328	0.72	+0.7
3,459,360	+	gspG	b3328	0.72	+0.4
3,459,368	-	gspG	b3328	0.74	+0.2
3,459,371	+	gspG	b3328	0.74	-0.1
3,459,389	-	gspG	b3328	0.79	-1.7
3,459,391	+	gspG	b3328	0.79	+1.2
3,459,391	+	gspG	b3328	0.79	+1.0
3,459,391	+	gspG	b3328	0.79	+0.5
3,459,399	-	gspG	b3328	0.81	+0.3
3,459,421	+	gspG	b3328	0.86	+1.3
3,459,446	-				+0.1
3,459,460	-				-2.4
3,459,460	-				+0.1
3,459,519	-				-1.0
3,459,519	-				+1.3
3,459,530	-				-0.8
3,459,530	-				-0.3
3,459,540	-				-0.5
3,459,570	+	gspH	b3329	0.16	-0.6
3,459,578	-	gspH	b3329	0.17	+0.0
3,459,603	-	gspH	b3329	0.22	-1.4
3,459,607	-	gspH	b3329	0.23	+0.5
3,459,607	-	gspH	b3329	0.23	+0.1
3,459,674	-	gspH	b3329	0.36	-0.8
3,459,698	-	gspH	b3329	0.41	+0.5
3,459,716	+	gspH	b3329	0.44	+0.6
3,459,742	+	gspH	b3329	0.49	-0.9
3,459,742	+	gspH	b3329	0.49	+1.0
3,459,747	+	gspH	b3329	0.50	+0.1
3,459,818	+	gspH	b3329	0.64	-0.2
3,459,851	-	gspH	b3329	0.71	+0.0
3,459,851	-	gspH	b3329	0.71	+0.7
3,459,893	+	gspH	b3329	0.79	+0.1
3,459,920	-	gspH	b3329	0.84	+0.2
3,459,930	+	gspH	b3329	0.86	+0.3
3,459,937	+	gspH	b3329	0.88	-0.1
3,459,937	+	gspH	b3329	0.88	+0.4
3,459,947	+	gspH	b3329	0.90	+0.3
3,459,947	+	gspH	b3329	0.90	-0.0
3,459,955	-				-0.2
3,459,964	-				-0.0
3,459,978	+				-0.2
3,459,978	+				-0.4
3,459,978	+				+1.2
3,459,995	+				-1.4
3,460,012	+				-1.0
3,460,012	+				-0.1
3,460,031	-				-0.1
3,460,043	-	yheH	b3330	0.12	-0.2
3,460,057	+	yheH	b3330	0.16	-1.6
3,460,081	+	yheH	b3330	0.22	-0.8
3,460,081	+	yheH	b3330	0.22	-1.1
3,460,081	+	yheH	b3330	0.22	-0.7
3,460,081	+	yheH	b3330	0.22	-0.5
3,460,083	+	yheH	b3330	0.23	+0.8
3,460,083	+	yheH	b3330	0.23	-0.1
3,460,086	-	yheH	b3330	0.24	-0.9
3,460,086	-	yheH	b3330	0.24	+0.4
3,460,086	-	yheH	b3330	0.24	-0.3
3,460,089	-	yheH	b3330	0.25	+0.3
3,460,089	-	yheH	b3330	0.25	+0.3
3,460,107	+	yheH	b3330	0.29	-0.0
3,460,114	+	yheH	b3330	0.31	-0.2
3,460,133	+	yheH	b3330	0.36	-0.2
3,460,198	-	yheH	b3330	0.53	-0.1
3,460,198	-	yheH	b3330	0.53	-1.1
3,460,224	+	yheH	b3330	0.60	-0.5
3,460,248	-	yheH	b3330	0.67	+0.5
3,460,248	-	yheH	b3330	0.67	-0.1
3,460,248	-	yheH	b3330	0.67	+0.3
3,460,277	-	yheH	b3330	0.74	+0.7
3,460,280	+	yheH	b3330	0.75	-0.3
3,460,280	+	yheH	b3330	0.75	-0.1
3,460,280	+	yheH	b3330	0.75	-1.1
3,460,280	+	yheH	b3330	0.75	-0.8
3,460,280	+	yheH	b3330	0.75	-0.4
3,460,385	+				-0.1
3,460,388	-				-1.3
3,460,395	+				-0.4
3,460,395	+				-0.3
3,460,401	+				+0.2
3,460,439	-	gspJ	b3331	0.12	+0.8
3,460,518	+				+3.0
3,460,524	+	gspJ	b3331	0.27	-0.4
3,460,529	-	gspJ	b3331	0.28	+0.5
3,460,534	-	gspJ	b3331	0.29	-0.4
3,460,538	-	gspJ	b3331	0.29	-0.0
3,460,569	-	gspJ	b3331	0.35	-0.6
3,460,569	-	gspJ	b3331	0.35	-1.4
3,460,580	-	gspJ	b3331	0.36	+0.6
3,460,592	-	gspJ	b3331	0.38	-0.3
3,460,596	+	gspJ	b3331	0.39	-0.6
3,460,639	-	gspJ	b3331	0.46	-0.2
3,460,666	+				-2.1
3,460,667	+	gspJ	b3331	0.51	+0.2
3,460,668	-	gspJ	b3331	0.51	+0.4
3,460,674	-	gspJ	b3331	0.52	-0.3
3,460,686	-	gspJ	b3331	0.54	+0.2
3,460,686	-	gspJ	b3331	0.54	+0.5
3,460,705	-	gspJ	b3331	0.58	+0.6
3,460,705	-	gspJ	b3331	0.58	-3.4
3,460,707	+	gspJ	b3331	0.58	+1.2
3,460,713	-	gspJ	b3331	0.59	-0.2
3,460,713	-	gspJ	b3331	0.59	-0.4
3,460,725	-	gspJ	b3331	0.61	+0.0
3,460,725	-	gspJ	b3331	0.61	+0.9
3,460,727	-	gspJ	b3331	0.61	+0.2
3,460,732	-	gspJ	b3331	0.62	-0.6
3,460,734	-	gspJ	b3331	0.63	+1.3
3,460,770	-	gspJ	b3331	0.69	+0.0
3,460,770	-	gspJ	b3331	0.69	-0.6
3,460,770	-	gspJ	b3331	0.69	+1.0
3,460,771	-	gspJ	b3331	0.69	+0.0
3,460,835	+	gspJ	b3331	0.80	+0.4
3,460,835	+	gspJ	b3331	0.80	+1.0
3,460,848	+	gspJ	b3331	0.82	-0.4
3,460,848	+	gspJ	b3331	0.82	-0.1
3,460,854	+	gspJ	b3331	0.83	+0.1
3,460,870	-	gspJ	b3331	0.86	-0.1
3,460,872	-	gspJ	b3331	0.86	-0.9
3,460,872	-	gspJ	b3331	0.86	-0.6
3,460,877	+	gspJ	b3331	0.87	+0.3
3,460,890	-	gspJ	b3331	0.89	-0.7
3,460,900	+				+0.3
3,460,900	+				-0.3
3,460,937	-				-0.5
3,460,937	-				-0.3
3,460,978	-				+1.7
3,460,978	-				-1.1
3,460,982	+				-0.1
3,460,982	+				-0.1