Strain Fitness in Escherichia coli BW25113 around b2933

Experiment: vcDART vector transformation; agar assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	vcDART vector transformation; agar assay
3,074,517	-	yggP	b4465	0.25	+0.3
3,074,768	-	yggP	b4465	0.44	-0.3
3,074,776	+	yggP	b4465	0.45	-0.6
3,074,819	+	yggP	b4465	0.48	-0.1
3,074,820	+	yggP	b4465	0.48	+0.4
3,074,820	+	yggP	b4465	0.48	-0.2
3,074,840	+	yggP	b4465	0.50	+0.4
3,074,883	-	yggP	b4465	0.53	+0.0
3,074,893	-	yggP	b4465	0.54	-0.8
3,074,908	+	yggP	b4465	0.55	-0.1
3,074,908	+	yggP	b4465	0.55	-0.1
3,074,908	+	yggP	b4465	0.55	-1.1
3,074,923	+	yggP	b4465	0.56	+0.1
3,074,931	-	yggP	b4465	0.57	-0.9
3,074,931	-	yggP	b4465	0.57	-0.1
3,074,941	-	yggP	b4465	0.58	-1.4
3,074,941	-	yggP	b4465	0.58	-0.5
3,074,958	+	yggP	b4465	0.59	-0.2
3,074,961	-	yggP	b4465	0.59	-0.6
3,074,975	-	yggP	b4465	0.61	-0.7
3,074,979	-	yggP	b4465	0.61	+0.2
3,075,013	+	yggP	b4465	0.64	+0.3
3,075,016	-	yggP	b4465	0.64	-1.1
3,075,022	+	yggP	b4465	0.64	-0.4
3,075,022	+	yggP	b4465	0.64	-0.6
3,075,022	+	yggP	b4465	0.64	-0.0
3,075,024	+	yggP	b4465	0.64	-1.1
3,075,024	+	yggP	b4465	0.64	+0.2
3,075,024	+	yggP	b4465	0.64	+0.6
3,075,025	+	yggP	b4465	0.64	+0.3
3,075,030	-	yggP	b4465	0.65	-0.1
3,075,033	+	yggP	b4465	0.65	-0.3
3,075,034	+	yggP	b4465	0.65	-1.1
3,075,034	+	yggP	b4465	0.65	-1.6
3,075,034	+	yggP	b4465	0.65	-0.2
3,075,034	+	yggP	b4465	0.65	+0.2
3,075,034	+	yggP	b4465	0.65	-0.5
3,075,035	+	yggP	b4465	0.65	-0.8
3,075,039	+	yggP	b4465	0.66	-0.7
3,075,042	-	yggP	b4465	0.66	+0.9
3,075,042	-	yggP	b4465	0.66	+1.6
3,075,074	+	yggP	b4465	0.68	-0.4
3,075,074	+	yggP	b4465	0.68	-0.1
3,075,074	+	yggP	b4465	0.68	+0.6
3,075,077	+	yggP	b4465	0.69	-0.2
3,075,077	+	yggP	b4465	0.69	+0.9
3,075,126	+	yggP	b4465	0.72	-0.7
3,075,168	+	yggP	b4465	0.76	-0.7
3,075,179	+	yggP	b4465	0.77	-0.1
3,075,179	+	yggP	b4465	0.77	-0.5
3,075,187	-	yggP	b4465	0.77	-1.4
3,075,258	-	yggP	b4465	0.83	-0.4
3,075,262	+	yggP	b4465	0.83	-0.7
3,075,269	-	yggP	b4465	0.84	-1.1
3,075,277	-	yggP	b4465	0.84	+0.8
3,075,317	-	yggP	b4465	0.87	-0.3
3,075,322	+	yggP	b4465	0.88	+0.3
3,075,325	+	yggP	b4465	0.88	-0.6
3,075,325	+	yggP	b4465	0.88	-0.4
3,075,326	-	yggP	b4465	0.88	+0.6
3,075,328	-	yggP	b4465	0.88	+0.3
3,075,330	+	yggP	b4465	0.88	-0.3
3,075,330	+	yggP	b4465	0.88	-1.3
3,075,330	-	yggP	b4465	0.88	+3.5
3,075,332	+	yggP	b4465	0.88	+0.1
3,075,333	-	yggP	b4465	0.89	+1.7
3,075,333	-	yggP	b4465	0.89	+1.3
3,075,335	+	yggP	b4465	0.89	+0.2
3,075,335	+	yggP	b4465	0.89	+0.6
3,075,335	+	yggP	b4465	0.89	+1.2
3,075,337	-	yggP	b4465	0.89	-1.6
3,075,337	-	yggP	b4465	0.89	-1.5
3,075,338	-	yggP	b4465	0.89	-0.6
3,075,343	+	yggP	b4465	0.89	-1.3
3,075,343	+	yggP	b4465	0.89	-0.8
3,075,347	+	yggP	b4465	0.90	-1.1
3,075,347	+	yggP	b4465	0.90	-0.5
3,075,348	+	yggP	b4465	0.90	-0.7
3,075,348	+	yggP	b4465	0.90	-0.6
3,075,355	-				-0.3
3,075,376	+				+2.9
3,075,418	+				+1.9
3,075,426	-				-1.1
3,075,449	+				+0.7
3,075,494	-				-0.6
3,075,504	-				-2.1
3,075,504	-				-0.3
3,075,504	-				-0.4
3,075,504	-				-2.0
3,075,556	-				-0.4
3,075,559	-				-1.1
3,075,583	-				-0.8
3,075,611	-				-1.1
3,075,611	-				-0.5
3,075,611	-				-0.9
3,075,611	-				-0.4
3,075,611	-				+0.3
3,075,612	-				-0.7
3,075,612	-				-0.6
3,075,621	-				-0.0
3,075,621	-				-1.0
3,075,621	-				-1.4
3,075,626	-				-0.5
3,075,626	-				-1.9
3,075,627	-				-1.9
3,075,631	-				-1.2
3,075,639	-	cmtA	b2933	0.11	-0.6
3,075,659	+	cmtA	b2933	0.12	+1.9
3,075,719	-	cmtA	b2933	0.16	-1.7
3,075,731	+	cmtA	b2933	0.17	+0.9
3,075,731	+	cmtA	b2933	0.17	+1.9
3,075,781	-	cmtA	b2933	0.21	-0.9
3,075,825	-	cmtA	b2933	0.24	-0.3
3,075,906	+	cmtA	b2933	0.30	-1.1
3,075,912	-	cmtA	b2933	0.30	-0.4
3,075,916	-	cmtA	b2933	0.30	+0.2
3,075,918	-	cmtA	b2933	0.31	-1.5
3,075,926	-	cmtA	b2933	0.31	-0.5
3,075,926	-	cmtA	b2933	0.31	-1.2
3,075,943	+	cmtA	b2933	0.32	-1.2
3,075,971	-	cmtA	b2933	0.34	-0.6
3,076,009	-	cmtA	b2933	0.37	-0.4
3,076,016	-	cmtA	b2933	0.38	-0.0
3,076,016	-	cmtA	b2933	0.38	+1.1
3,076,034	-	cmtA	b2933	0.39	+0.0
3,076,037	+	cmtA	b2933	0.39	-0.2
3,076,037	+	cmtA	b2933	0.39	-1.0
3,076,037	+	cmtA	b2933	0.39	-0.9
3,076,045	-	cmtA	b2933	0.40	+0.1
3,076,045	-	cmtA	b2933	0.40	+0.3
3,076,051	-	cmtA	b2933	0.40	+0.2
3,076,051	-	cmtA	b2933	0.40	-0.0
3,076,084	+	cmtA	b2933	0.43	-0.6
3,076,122	-	cmtA	b2933	0.45	-1.3
3,076,165	-	cmtA	b2933	0.48	-0.5
3,076,167	+	cmtA	b2933	0.49	-2.0
3,076,167	+	cmtA	b2933	0.49	+1.1
3,076,180	-	cmtA	b2933	0.49	-1.7
3,076,195	+	cmtA	b2933	0.51	-0.2
3,076,195	+	cmtA	b2933	0.51	-0.3
3,076,200	+	cmtA	b2933	0.51	-0.7
3,076,217	+	cmtA	b2933	0.52	-0.5
3,076,225	-	cmtA	b2933	0.53	-1.8
3,076,226	-	cmtA	b2933	0.53	-0.2
3,076,230	+	cmtA	b2933	0.53	-1.1
3,076,237	+	cmtA	b2933	0.54	-0.3
3,076,244	+	cmtA	b2933	0.54	-1.2
3,076,244	+	cmtA	b2933	0.54	-0.4
3,076,281	-	cmtA	b2933	0.57	-0.3
3,076,317	-	cmtA	b2933	0.59	-0.1
3,076,340	-	cmtA	b2933	0.61	-0.4
3,076,351	-	cmtA	b2933	0.62	+0.1
3,076,351	-	cmtA	b2933	0.62	-0.8
3,076,355	+	cmtA	b2933	0.62	-1.6
3,076,360	-	cmtA	b2933	0.62	-0.2
3,076,363	+	cmtA	b2933	0.63	-0.1
3,076,363	+	cmtA	b2933	0.63	-1.2
3,076,385	-	cmtA	b2933	0.64	-0.4
3,076,386	-	cmtA	b2933	0.64	-1.0
3,076,390	-	cmtA	b2933	0.65	-0.2
3,076,426	+	cmtA	b2933	0.67	+1.3
3,076,449	+	cmtA	b2933	0.69	-0.2
3,076,449	+	cmtA	b2933	0.69	-1.7
3,076,512	+	cmtA	b2933	0.73	-0.5
3,076,566	+	cmtA	b2933	0.77	-0.9
3,076,566	+	cmtA	b2933	0.77	-0.4
3,076,566	+	cmtA	b2933	0.77	-1.0
3,076,574	-	cmtA	b2933	0.78	-0.0
3,076,598	-	cmtA	b2933	0.80	-1.6
3,076,620	+	cmtA	b2933	0.81	-0.9
3,076,630	+	cmtA	b2933	0.82	+0.2
3,076,630	-	cmtA	b2933	0.82	-0.3
3,076,636	+	cmtA	b2933	0.82	-0.4
3,076,663	+	cmtA	b2933	0.84	-0.5
3,076,668	+	cmtA	b2933	0.85	-1.2
3,076,668	+	cmtA	b2933	0.85	+0.1
3,076,676	+	cmtA	b2933	0.85	-0.8
3,076,676	+	cmtA	b2933	0.85	-0.3
3,076,676	+	cmtA	b2933	0.85	-2.4
3,076,677	+	cmtA	b2933	0.85	-0.1
3,076,677	+	cmtA	b2933	0.85	+0.5
3,076,701	+	cmtA	b2933	0.87	-1.9
3,076,750	+				+1.6
3,076,750	+				-1.2
3,076,754	+				+1.3
3,076,754	+				-0.1
3,076,813	-				-0.1
3,076,837	+				+1.9
3,076,837	-				+0.2
3,076,841	+				-0.9
3,076,853	+				-0.1
3,076,854	+				+0.9
3,076,854	+				+0.9
3,077,141	-	cmtB	b2934	0.52	+1.9
3,077,180	+	cmtB	b2934	0.61	+1.9
3,077,305	+	cmtB	b2934	0.89	+1.2
3,077,305	+	cmtB	b2934	0.89	-0.1
3,077,438	-				-0.2
3,077,496	-				-1.4
3,077,496	-				-0.5
3,077,516	+				+0.0
3,077,531	+				-0.1
3,077,531	+				-1.8
3,077,576	+				+1.4
3,077,596	-				-0.1
3,077,596	-				-0.1
3,077,617	-				-0.4
3,077,695	-				+0.4
3,077,707	-				-0.1
3,077,717	-				+0.6
3,077,778	-				+0.9
3,077,778	-				+1.9