Strain Fitness in Escherichia coli BW25113 around b1670

Experiment: BHIS with Methotrexate 1.98 mM 24 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhS and ydhT are separated by 11 nucleotidesydhT and ydhU are separated by 3 nucleotidesydhU and ydhX overlap by 4 nucleotidesydhX and ydhW are separated by 63 nucleotides b1668: ydhS - conserved protein with FAD/NAD(P)-binding domain (NCBI), at 1,745,155 to 1,746,759 ydhS b1669: ydhT - hypothetical protein (NCBI), at 1,746,771 to 1,747,583 ydhT b1670: ydhU - predicted cytochrome (NCBI), at 1,747,587 to 1,748,372 ydhU b1671: ydhX - predicted 4Fe-4S ferridoxin-type protein (RefSeq), at 1,748,369 to 1,749,037 ydhX b1672: ydhW - hypothetical protein (NCBI), at 1,749,101 to 1,749,748 ydhW Position (kb) 1747 1748 1749Strain fitness (log2 ratio) -1 0 1at 1746.854 kb on - strand, within ydhTat 1746.877 kb on + strand, within ydhTat 1746.969 kb on + strand, within ydhTat 1747.895 kb on - strand, within ydhUat 1747.958 kb on - strand, within ydhUat 1747.958 kb on - strand, within ydhUat 1747.965 kb on - strand, within ydhUat 1748.076 kb on - strand, within ydhUat 1748.231 kb on - strand, within ydhUat 1748.231 kb on - strand, within ydhUat 1748.764 kb on - strand, within ydhXat 1748.764 kb on - strand, within ydhXat 1748.847 kb on - strand, within ydhXat 1749.057 kb on - strandat 1749.057 kb on - strandat 1749.123 kb on + strandat 1749.144 kb on + strandat 1749.151 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 24 hr
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1,746,854 - ydhT b1669 0.10 -0.4
1,746,877 + ydhT b1669 0.13 -0.1
1,746,969 + ydhT b1669 0.24 +0.2
1,747,895 - ydhU b1670 0.39 +0.4
1,747,958 - ydhU b1670 0.47 -0.4
1,747,958 - ydhU b1670 0.47 +0.0
1,747,965 - ydhU b1670 0.48 +0.9
1,748,076 - ydhU b1670 0.62 -0.1
1,748,231 - ydhU b1670 0.82 +0.7
1,748,231 - ydhU b1670 0.82 +1.0
1,748,764 - ydhX b1671 0.59 +0.0
1,748,764 - ydhX b1671 0.59 +0.4
1,748,847 - ydhX b1671 0.71 +0.7
1,749,057 - +0.2
1,749,057 - -0.5
1,749,123 + -0.2
1,749,144 + +0.1
1,749,151 - -0.1

Or see this region's nucleotide sequence