Experiment: BHIS with Methotrexate 1.98 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaA and moaB are separated by 21 nucleotides moaB and moaC are separated by 2 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 1 nucleotides
b0781: moaA - molybdenum cofactor biosynthesis protein A (NCBI), at 816,267 to 817,256
moaA
b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790
moaB
b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278
moaC
b0784: moaD - molybdopterin synthase, small subunit (NCBI), at 818,271 to 818,516
moaD
b0785: moaE - molybdopterin synthase, large subunit (NCBI), at 818,518 to 818,970
moaE
Position (kb) 817
818 Strain fitness (log2 ratio) -2
-1
0
1 at 816.333 kb on - strand at 816.363 kb on + strand at 816.363 kb on + strand at 816.378 kb on - strand, within moaA at 816.609 kb on - strand, within moaA at 816.624 kb on + strand, within moaA at 816.680 kb on + strand, within moaA at 816.746 kb on + strand, within moaA at 816.746 kb on + strand, within moaA at 816.782 kb on - strand, within moaA at 816.817 kb on + strand, within moaA at 816.912 kb on + strand, within moaA at 817.009 kb on - strand, within moaA at 817.009 kb on - strand, within moaA at 817.049 kb on - strand, within moaA at 817.085 kb on - strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.280 kb on - strand at 817.280 kb on - strand at 817.355 kb on - strand, within moaB at 817.485 kb on + strand, within moaB at 817.485 kb on + strand, within moaB at 817.818 kb on - strand at 817.818 kb on - strand at 817.852 kb on - strand, within moaC at 817.852 kb on - strand, within moaC at 817.872 kb on - strand, within moaC at 817.912 kb on + strand, within moaC at 817.912 kb on + strand, within moaC at 818.006 kb on - strand, within moaC at 818.012 kb on - strand, within moaC at 818.167 kb on - strand, within moaC at 818.211 kb on + strand, within moaC at 818.219 kb on - strand, within moaC at 818.219 kb on - strand, within moaC at 818.252 kb on + strand at 818.252 kb on + strand at 818.369 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.677 kb on - strand, within moaE at 818.687 kb on - strand, within moaE at 818.764 kb on - strand, within moaE
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 24 hr remove 816,333 - +0.1 816,363 + -1.4 816,363 + -0.3 816,378 - moaA b0781 0.11 -0.9 816,609 - moaA b0781 0.35 -2.0 816,624 + moaA b0781 0.36 -0.1 816,680 + moaA b0781 0.42 -0.0 816,746 + moaA b0781 0.48 -0.7 816,746 + moaA b0781 0.48 -0.4 816,782 - moaA b0781 0.52 -1.4 816,817 + moaA b0781 0.56 -0.5 816,912 + moaA b0781 0.65 -0.7 817,009 - moaA b0781 0.75 -0.4 817,009 - moaA b0781 0.75 -1.6 817,049 - moaA b0781 0.79 -0.4 817,085 - moaA b0781 0.83 -0.1 817,105 + moaA b0781 0.85 +0.8 817,105 + moaA b0781 0.85 -0.1 817,105 + moaA b0781 0.85 -0.8 817,280 - -0.1 817,280 - -0.1 817,355 - moaB b0782 0.15 -0.0 817,485 + moaB b0782 0.40 +0.9 817,485 + moaB b0782 0.40 +0.2 817,818 - -0.4 817,818 - -0.2 817,852 - moaC b0783 0.12 -0.7 817,852 - moaC b0783 0.12 -0.4 817,872 - moaC b0783 0.16 -1.5 817,912 + moaC b0783 0.24 -0.7 817,912 + moaC b0783 0.24 -0.5 818,006 - moaC b0783 0.44 -0.0 818,012 - moaC b0783 0.45 +0.7 818,167 - moaC b0783 0.77 -0.5 818,211 + moaC b0783 0.86 -0.9 818,219 - moaC b0783 0.88 -0.7 818,219 - moaC b0783 0.88 -0.7 818,252 + -2.0 818,252 + +0.3 818,369 + moaD b0784 0.40 -0.5 818,455 + moaD b0784 0.75 -0.3 818,455 + moaD b0784 0.75 -0.4 818,566 - moaE b0785 0.11 -0.8 818,566 - moaE b0785 0.11 -0.1 818,566 - moaE b0785 0.11 -0.2 818,677 - moaE b0785 0.35 +0.2 818,687 - moaE b0785 0.37 -0.8 818,764 - moaE b0785 0.54 -0.9
Or see this region's nucleotide sequence