Experiment: BHIS with Methotrexate 1.98 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ampH and sbmA are separated by 351 nucleotides sbmA and yaiW are separated by 12 nucleotides
b0376: ampH - beta-lactamase/D-alanine carboxypeptidase (NCBI), at 394,354 to 395,511
ampH
b0377: sbmA - predicted transporter (NCBI), at 395,863 to 397,083
sbmA
b0378: yaiW - predicted DNA-binding transcriptional regulator (NCBI), at 397,096 to 398,190
yaiW
Position (kb) 395
396
397
398 Strain fitness (log2 ratio) -2
-1
0
1 at 394.909 kb on - strand, within ampH at 394.942 kb on - strand, within ampH at 394.942 kb on - strand, within ampH at 394.942 kb on - strand, within ampH at 394.955 kb on + strand, within ampH at 394.956 kb on + strand, within ampH at 394.964 kb on - strand, within ampH at 394.964 kb on - strand, within ampH at 395.074 kb on - strand, within ampH at 395.200 kb on + strand, within ampH at 395.200 kb on + strand, within ampH at 395.208 kb on - strand, within ampH at 395.208 kb on - strand, within ampH at 395.223 kb on + strand, within ampH at 395.227 kb on + strand, within ampH at 395.227 kb on + strand, within ampH at 395.280 kb on - strand, within ampH at 395.298 kb on - strand, within ampH at 395.350 kb on + strand, within ampH at 395.404 kb on - strand at 395.534 kb on + strand at 395.535 kb on - strand at 395.536 kb on + strand at 395.536 kb on + strand at 395.557 kb on - strand at 395.557 kb on - strand at 395.579 kb on + strand at 395.667 kb on - strand at 395.672 kb on - strand at 395.674 kb on + strand at 395.686 kb on + strand at 395.740 kb on + strand at 395.759 kb on + strand at 395.787 kb on - strand at 395.793 kb on + strand at 395.798 kb on + strand at 395.798 kb on + strand at 395.818 kb on + strand at 395.821 kb on - strand at 395.826 kb on - strand at 395.839 kb on + strand at 395.847 kb on + strand at 395.862 kb on - strand at 395.960 kb on + strand at 396.000 kb on + strand, within sbmA at 396.114 kb on + strand, within sbmA at 396.114 kb on + strand, within sbmA at 396.126 kb on + strand, within sbmA at 396.136 kb on + strand, within sbmA at 396.136 kb on + strand, within sbmA at 396.136 kb on + strand, within sbmA at 396.178 kb on + strand, within sbmA at 396.202 kb on - strand, within sbmA at 396.207 kb on + strand, within sbmA at 396.207 kb on + strand, within sbmA at 396.216 kb on + strand, within sbmA at 396.291 kb on - strand, within sbmA at 396.291 kb on - strand, within sbmA at 396.309 kb on + strand, within sbmA at 396.349 kb on + strand, within sbmA at 396.349 kb on + strand, within sbmA at 396.359 kb on + strand, within sbmA at 396.359 kb on + strand, within sbmA at 396.386 kb on - strand, within sbmA at 396.437 kb on - strand, within sbmA at 396.580 kb on - strand, within sbmA at 396.580 kb on - strand, within sbmA at 396.586 kb on + strand, within sbmA at 396.623 kb on + strand, within sbmA at 396.691 kb on - strand, within sbmA at 396.765 kb on + strand, within sbmA at 396.775 kb on + strand, within sbmA at 396.819 kb on - strand, within sbmA at 396.881 kb on + strand, within sbmA at 396.882 kb on - strand, within sbmA at 396.882 kb on - strand, within sbmA at 396.900 kb on + strand, within sbmA at 396.904 kb on - strand, within sbmA at 396.907 kb on - strand, within sbmA at 396.907 kb on - strand, within sbmA at 396.911 kb on + strand, within sbmA at 397.014 kb on - strand at 397.014 kb on - strand at 397.025 kb on + strand at 397.033 kb on - strand at 397.066 kb on + strand at 397.163 kb on - strand at 397.163 kb on - strand at 397.165 kb on - strand at 397.225 kb on - strand, within yaiW at 397.330 kb on + strand, within yaiW at 397.421 kb on + strand, within yaiW at 397.557 kb on + strand, within yaiW at 397.557 kb on + strand, within yaiW at 397.565 kb on - strand, within yaiW at 397.619 kb on - strand, within yaiW at 397.730 kb on - strand, within yaiW at 397.730 kb on - strand, within yaiW at 397.736 kb on - strand, within yaiW at 397.761 kb on + strand, within yaiW at 397.802 kb on - strand, within yaiW at 397.804 kb on + strand, within yaiW at 397.818 kb on + strand, within yaiW at 397.847 kb on + strand, within yaiW at 397.847 kb on + strand, within yaiW at 397.891 kb on + strand, within yaiW at 397.987 kb on + strand, within yaiW at 398.006 kb on + strand, within yaiW at 398.025 kb on + strand, within yaiW at 398.025 kb on + strand, within yaiW
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 24 hr remove 394,909 - ampH b0376 0.48 -0.2 394,942 - ampH b0376 0.51 +0.6 394,942 - ampH b0376 0.51 -0.4 394,942 - ampH b0376 0.51 +0.6 394,955 + ampH b0376 0.52 -0.2 394,956 + ampH b0376 0.52 -0.1 394,964 - ampH b0376 0.53 +0.4 394,964 - ampH b0376 0.53 -0.1 395,074 - ampH b0376 0.62 -0.5 395,200 + ampH b0376 0.73 -0.1 395,200 + ampH b0376 0.73 +0.2 395,208 - ampH b0376 0.74 -0.3 395,208 - ampH b0376 0.74 -0.5 395,223 + ampH b0376 0.75 -0.4 395,227 + ampH b0376 0.75 -0.4 395,227 + ampH b0376 0.75 -1.0 395,280 - ampH b0376 0.80 +0.2 395,298 - ampH b0376 0.82 +0.2 395,350 + ampH b0376 0.86 +0.0 395,404 - -0.2 395,534 + -0.8 395,535 - -0.4 395,536 + -0.2 395,536 + -0.3 395,557 - -0.1 395,557 - -0.5 395,579 + +0.3 395,667 - -0.2 395,672 - -0.5 395,674 + -1.2 395,686 + -1.1 395,740 + -1.4 395,759 + -1.2 395,787 - +0.1 395,793 + -0.8 395,798 + -1.1 395,798 + -1.6 395,818 + -1.9 395,821 - -0.5 395,826 - -0.3 395,839 + +0.1 395,847 + -1.0 395,862 - -0.2 395,960 + -0.2 396,000 + sbmA b0377 0.11 -0.3 396,114 + sbmA b0377 0.21 +1.5 396,114 + sbmA b0377 0.21 +0.2 396,126 + sbmA b0377 0.22 +0.3 396,136 + sbmA b0377 0.22 +0.5 396,136 + sbmA b0377 0.22 +0.2 396,136 + sbmA b0377 0.22 +0.3 396,178 + sbmA b0377 0.26 -0.0 396,202 - sbmA b0377 0.28 +0.4 396,207 + sbmA b0377 0.28 -1.1 396,207 + sbmA b0377 0.28 +0.3 396,216 + sbmA b0377 0.29 -0.2 396,291 - sbmA b0377 0.35 +0.4 396,291 - sbmA b0377 0.35 +0.7 396,309 + sbmA b0377 0.37 +0.2 396,349 + sbmA b0377 0.40 +0.2 396,349 + sbmA b0377 0.40 -0.5 396,359 + sbmA b0377 0.41 -0.2 396,359 + sbmA b0377 0.41 +0.2 396,386 - sbmA b0377 0.43 +0.2 396,437 - sbmA b0377 0.47 +0.5 396,580 - sbmA b0377 0.59 -0.1 396,580 - sbmA b0377 0.59 -0.5 396,586 + sbmA b0377 0.59 -0.2 396,623 + sbmA b0377 0.62 +0.7 396,691 - sbmA b0377 0.68 -0.2 396,765 + sbmA b0377 0.74 +0.0 396,775 + sbmA b0377 0.75 -0.1 396,819 - sbmA b0377 0.78 +0.1 396,881 + sbmA b0377 0.83 +0.4 396,882 - sbmA b0377 0.83 +0.8 396,882 - sbmA b0377 0.83 +0.4 396,900 + sbmA b0377 0.85 +0.8 396,904 - sbmA b0377 0.85 +1.0 396,907 - sbmA b0377 0.86 +0.4 396,907 - sbmA b0377 0.86 -0.1 396,911 + sbmA b0377 0.86 +0.3 397,014 - +0.0 397,014 - -0.2 397,025 + -0.3 397,033 - +0.0 397,066 + -0.4 397,163 - +0.0 397,163 - -1.0 397,165 - -0.8 397,225 - yaiW b0378 0.12 +0.0 397,330 + yaiW b0378 0.21 +0.3 397,421 + yaiW b0378 0.30 +0.2 397,557 + yaiW b0378 0.42 -0.4 397,557 + yaiW b0378 0.42 -0.2 397,565 - yaiW b0378 0.43 -0.9 397,619 - yaiW b0378 0.48 +0.7 397,730 - yaiW b0378 0.58 -0.3 397,730 - yaiW b0378 0.58 +0.2 397,736 - yaiW b0378 0.58 +0.4 397,761 + yaiW b0378 0.61 +0.2 397,802 - yaiW b0378 0.64 -0.1 397,804 + yaiW b0378 0.65 +0.4 397,818 + yaiW b0378 0.66 -0.1 397,847 + yaiW b0378 0.69 +0.1 397,847 + yaiW b0378 0.69 +0.2 397,891 + yaiW b0378 0.73 +0.1 397,987 + yaiW b0378 0.81 -0.0 398,006 + yaiW b0378 0.83 +0.2 398,025 + yaiW b0378 0.85 +0.2 398,025 + yaiW b0378 0.85 -0.2
Or see this region's nucleotide sequence