Strain Fitness in Escherichia coli BW25113 around b2003

Experiment: M9 with Methotrexate 0.1 mM 24 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Methotrexate 0.1 mM 24 hr
2,073,877	-	b2001	b2001	0.70	+0.5
2,073,890	+	b2001	b2001	0.71	+0.1
2,073,890	+	b2001	b2001	0.71	+0.9
2,074,027	+	b2001	b2001	0.80	-0.4
2,074,108	-	b2001	b2001	0.85	-0.9
2,074,258	+				+0.3
2,074,258	+				-0.4
2,074,262	+				-0.1
2,074,319	+				-0.1
2,074,396	+	yeeS	b2002	0.14	-0.1
2,074,498	+	yeeS	b2002	0.37	-0.0
2,074,527	+	yeeS	b2002	0.44	-0.4
2,074,527	+	yeeS	b2002	0.44	-0.4
2,074,565	-	yeeS	b2002	0.52	-0.2
2,074,622	+	yeeS	b2002	0.65	-0.2
2,074,649	+	yeeS	b2002	0.71	-0.0
2,074,657	-	yeeS	b2002	0.73	-0.5
2,074,690	+	yeeS	b2002	0.80	+0.7
2,074,696	+	yeeS	b2002	0.81	+0.5
2,074,696	-	yeeS	b2002	0.81	-0.1
2,074,782	+				-0.1
2,074,865	+	yeeT	b2003	0.11	+0.9
2,074,920	-	yeeT	b2003	0.36	-0.1
2,074,949	-	yeeT	b2003	0.49	-0.0
2,074,949	-	yeeT	b2003	0.49	+0.5
2,075,051	+				-0.1
2,075,051	+				+0.3
2,075,051	+				-0.3
2,075,199	-	yeeU	b2004	0.17	-1.5
2,075,199	-	yeeU	b2004	0.17	-0.1
2,075,201	+	yeeU	b2004	0.18	-0.6
2,075,209	+	yeeU	b2004	0.20	-0.2
2,075,302	+	yeeU	b2004	0.45	+0.1
2,075,407	+	yeeU	b2004	0.73	-0.2
2,075,416	+	yeeU	b2004	0.76	-0.5
2,075,452	-	yeeU	b2004	0.86	-0.0
2,075,452	-	yeeU	b2004	0.86	-0.2
2,075,642	+	yeeV	b2005	0.13	-1.2
2,075,642	+	yeeV	b2005	0.13	-0.2
2,075,827	+	yeeV	b2005	0.62	+0.2
2,075,848	-	yeeV	b2005	0.68	+0.1
2,075,848	-	yeeV	b2005	0.68	+0.1
2,075,933	+				+0.1
2,076,005	+	yeeW	b2006	0.24	+0.1
2,076,019	+	yeeW	b2006	0.33	-0.2
2,076,019	+	yeeW	b2006	0.33	+0.0