Experiment: M9 with Methotrexate 0.1 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcL and ybcM are separated by 9 nucleotides ybcM and ylcH are separated by 116 nucleotides ylcH and ybcN overlap by 4 nucleotides ybcN and ninE overlap by 1 nucleotides ninE and ybcO overlap by 8 nucleotides
b0545: ybcL - DLP12 prophage; predicted kinase inhibitor (NCBI), at 570,116 to 570,667
ybcL
b0546: ybcM - DLP12 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 570,677 to 571,474
ybcM
b4588: ylcH - hypothetical protein, DLP12 prophage (RefSeq), at 571,591 to 571,692
ylcH
b0547: ybcN - DLP12 prophage; predicted protein (NCBI), at 571,689 to 572,144
ybcN
b0548: ninE - DLP12 prophage; conserved protein (NCBI), at 572,144 to 572,314
ninE
b0549: ybcO - DLP12 prophage; predicted protein (NCBI), at 572,307 to 572,597
ybcO
Position (kb) 570
571
572 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 569.954 kb on - strand at 569.991 kb on - strand at 569.991 kb on - strand at 570.177 kb on - strand, within ybcL at 570.182 kb on - strand, within ybcL at 570.217 kb on + strand, within ybcL at 570.217 kb on - strand, within ybcL at 570.225 kb on - strand, within ybcL at 570.235 kb on - strand, within ybcL at 570.236 kb on - strand, within ybcL at 570.259 kb on + strand, within ybcL at 570.265 kb on - strand, within ybcL at 570.267 kb on - strand, within ybcL at 570.273 kb on - strand, within ybcL at 570.276 kb on + strand, within ybcL at 570.283 kb on - strand, within ybcL at 570.289 kb on - strand, within ybcL at 570.289 kb on - strand at 570.293 kb on + strand, within ybcL at 570.295 kb on + strand, within ybcL at 570.296 kb on + strand, within ybcL at 570.302 kb on - strand, within ybcL at 570.302 kb on - strand, within ybcL at 570.305 kb on + strand, within ybcL at 570.305 kb on + strand, within ybcL at 570.312 kb on + strand, within ybcL at 570.323 kb on + strand, within ybcL at 570.323 kb on + strand, within ybcL at 570.323 kb on + strand, within ybcL at 570.332 kb on - strand, within ybcL at 570.342 kb on + strand, within ybcL at 570.343 kb on + strand, within ybcL at 570.343 kb on + strand, within ybcL at 570.344 kb on + strand, within ybcL at 570.350 kb on - strand, within ybcL at 570.351 kb on - strand, within ybcL at 570.354 kb on - strand, within ybcL at 570.356 kb on + strand, within ybcL at 570.364 kb on + strand, within ybcL at 570.364 kb on + strand, within ybcL at 570.372 kb on - strand, within ybcL at 570.374 kb on + strand, within ybcL at 570.374 kb on + strand, within ybcL at 570.382 kb on - strand, within ybcL at 570.401 kb on + strand, within ybcL at 570.401 kb on + strand, within ybcL at 570.409 kb on + strand, within ybcL at 570.418 kb on + strand, within ybcL at 570.440 kb on + strand, within ybcL at 570.445 kb on + strand, within ybcL at 570.450 kb on + strand, within ybcL at 570.455 kb on + strand, within ybcL at 570.455 kb on + strand, within ybcL at 570.455 kb on + strand, within ybcL at 570.458 kb on - strand, within ybcL at 570.480 kb on + strand, within ybcL at 570.482 kb on + strand, within ybcL at 570.488 kb on - strand, within ybcL at 570.496 kb on + strand, within ybcL at 570.496 kb on + strand, within ybcL at 570.501 kb on + strand, within ybcL at 570.501 kb on + strand, within ybcL at 570.505 kb on + strand, within ybcL at 570.514 kb on + strand, within ybcL at 570.535 kb on + strand, within ybcL at 570.535 kb on + strand, within ybcL at 570.535 kb on + strand, within ybcL at 570.535 kb on + strand, within ybcL at 570.535 kb on + strand, within ybcL at 570.535 kb on + strand, within ybcL at 570.566 kb on + strand, within ybcL at 570.576 kb on + strand, within ybcL at 570.581 kb on + strand, within ybcL at 570.625 kb on + strand at 570.644 kb on + strand at 570.650 kb on + strand at 570.650 kb on + strand at 570.661 kb on + strand at 570.665 kb on - strand at 570.668 kb on + strand at 570.668 kb on + strand at 570.672 kb on + strand at 570.675 kb on - strand at 570.675 kb on - strand at 570.675 kb on - strand at 570.682 kb on + strand at 570.682 kb on + strand at 570.688 kb on + strand at 570.688 kb on + strand at 570.697 kb on - strand at 570.772 kb on + strand, within ybcM at 570.772 kb on + strand, within ybcM at 570.804 kb on + strand, within ybcM at 570.804 kb on + strand, within ybcM at 570.804 kb on + strand, within ybcM at 570.804 kb on + strand, within ybcM at 570.804 kb on + strand, within ybcM at 570.817 kb on + strand, within ybcM at 570.896 kb on + strand, within ybcM at 570.992 kb on + strand, within ybcM at 570.992 kb on + strand, within ybcM at 571.055 kb on + strand, within ybcM at 571.084 kb on - strand, within ybcM at 571.094 kb on - strand, within ybcM at 571.105 kb on + strand, within ybcM at 571.115 kb on + strand, within ybcM at 571.124 kb on - strand, within ybcM at 571.130 kb on - strand, within ybcM at 571.170 kb on - strand, within ybcM at 571.186 kb on + strand, within ybcM at 571.207 kb on + strand, within ybcM at 571.207 kb on + strand, within ybcM at 571.215 kb on - strand, within ybcM at 571.226 kb on + strand, within ybcM at 571.283 kb on + strand, within ybcM at 571.301 kb on + strand, within ybcM at 571.301 kb on + strand, within ybcM at 571.306 kb on + strand, within ybcM at 571.340 kb on + strand, within ybcM at 571.340 kb on + strand, within ybcM at 571.340 kb on + strand, within ybcM at 571.340 kb on + strand, within ybcM at 571.341 kb on - strand, within ybcM at 571.345 kb on - strand, within ybcM at 571.358 kb on - strand, within ybcM at 571.418 kb on + strand at 571.427 kb on + strand at 571.477 kb on - strand at 571.486 kb on - strand at 571.546 kb on - strand at 571.560 kb on - strand at 571.575 kb on - strand at 571.575 kb on - strand at 571.585 kb on - strand at 571.596 kb on + strand at 571.596 kb on - strand at 571.596 kb on - strand at 571.602 kb on - strand, within ylcH at 571.618 kb on + strand, within ylcH at 571.623 kb on - strand, within ylcH at 571.629 kb on + strand, within ylcH at 571.638 kb on - strand, within ylcH at 571.671 kb on + strand, within ylcH at 571.681 kb on + strand, within ylcH at 571.681 kb on + strand, within ylcH at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.687 kb on + strand at 571.694 kb on - strand at 571.695 kb on - strand at 571.752 kb on + strand, within ybcN at 571.752 kb on + strand, within ybcN at 571.909 kb on + strand, within ybcN at 571.931 kb on + strand, within ybcN at 571.937 kb on - strand, within ybcN at 571.939 kb on - strand, within ybcN at 571.939 kb on - strand, within ybcN at 572.055 kb on + strand, within ybcN at 572.068 kb on - strand, within ybcN at 572.179 kb on + strand, within ninE at 572.179 kb on + strand, within ninE at 572.179 kb on + strand, within ninE at 572.195 kb on - strand, within ninE at 572.214 kb on - strand, within ninE at 572.343 kb on - strand, within ybcO
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.1 mM 24 hr remove 569,954 - +0.3 569,991 - -0.4 569,991 - -0.4 570,177 - ybcL b0545 0.11 +2.2 570,182 - ybcL b0545 0.12 +1.6 570,217 + ybcL b0545 0.18 +1.2 570,217 - ybcL b0545 0.18 -0.7 570,225 - ybcL b0545 0.20 -0.8 570,235 - ybcL b0545 0.22 -1.1 570,236 - ybcL b0545 0.22 +1.2 570,259 + ybcL b0545 0.26 +1.2 570,265 - ybcL b0545 0.27 +1.7 570,267 - ybcL b0545 0.27 +1.6 570,273 - ybcL b0545 0.28 +1.2 570,276 + ybcL b0545 0.29 +1.2 570,283 - ybcL b0545 0.30 -0.4 570,289 - ybcL b0545 0.31 +0.3 570,289 - +1.8 570,293 + ybcL b0545 0.32 +1.2 570,295 + ybcL b0545 0.32 +0.2 570,296 + ybcL b0545 0.33 -0.7 570,302 - ybcL b0545 0.34 +0.7 570,302 - ybcL b0545 0.34 -0.2 570,305 + ybcL b0545 0.34 +1.2 570,305 + ybcL b0545 0.34 +1.2 570,312 + ybcL b0545 0.36 +0.3 570,323 + ybcL b0545 0.38 -2.0 570,323 + ybcL b0545 0.38 +0.2 570,323 + ybcL b0545 0.38 +1.2 570,332 - ybcL b0545 0.39 +1.2 570,342 + ybcL b0545 0.41 +0.9 570,343 + ybcL b0545 0.41 -0.2 570,343 + ybcL b0545 0.41 -0.4 570,344 + ybcL b0545 0.41 -0.7 570,350 - ybcL b0545 0.42 +0.2 570,351 - ybcL b0545 0.43 +1.2 570,354 - ybcL b0545 0.43 +1.2 570,356 + ybcL b0545 0.43 +0.2 570,364 + ybcL b0545 0.45 +0.3 570,364 + ybcL b0545 0.45 +0.2 570,372 - ybcL b0545 0.46 +1.2 570,374 + ybcL b0545 0.47 +1.0 570,374 + ybcL b0545 0.47 -0.4 570,382 - ybcL b0545 0.48 +1.2 570,401 + ybcL b0545 0.52 +0.6 570,401 + ybcL b0545 0.52 +0.3 570,409 + ybcL b0545 0.53 +1.5 570,418 + ybcL b0545 0.55 +0.1 570,440 + ybcL b0545 0.59 -0.3 570,445 + ybcL b0545 0.60 +0.9 570,450 + ybcL b0545 0.61 +0.8 570,455 + ybcL b0545 0.61 -0.4 570,455 + ybcL b0545 0.61 -0.9 570,455 + ybcL b0545 0.61 +1.6 570,458 - ybcL b0545 0.62 +1.2 570,480 + ybcL b0545 0.66 -0.1 570,482 + ybcL b0545 0.66 -0.0 570,488 - ybcL b0545 0.67 +1.2 570,496 + ybcL b0545 0.69 +0.1 570,496 + ybcL b0545 0.69 +0.3 570,501 + ybcL b0545 0.70 -0.4 570,501 + ybcL b0545 0.70 +0.8 570,505 + ybcL b0545 0.70 +0.2 570,514 + ybcL b0545 0.72 -0.3 570,535 + ybcL b0545 0.76 -0.3 570,535 + ybcL b0545 0.76 +0.1 570,535 + ybcL b0545 0.76 -0.2 570,535 + ybcL b0545 0.76 -0.6 570,535 + ybcL b0545 0.76 +0.9 570,535 + ybcL b0545 0.76 +0.2 570,566 + ybcL b0545 0.82 +1.0 570,576 + ybcL b0545 0.83 +0.1 570,581 + ybcL b0545 0.84 +0.2 570,625 + -0.8 570,644 + -1.4 570,650 + +0.3 570,650 + +0.7 570,661 + +0.3 570,665 - +1.2 570,668 + +3.5 570,668 + -0.8 570,672 + +0.3 570,675 - +1.2 570,675 - +1.2 570,675 - +1.2 570,682 + -1.4 570,682 + +0.5 570,688 + +2.2 570,688 + +0.2 570,697 - +0.2 570,772 + ybcM b0546 0.12 +0.4 570,772 + ybcM b0546 0.12 +0.3 570,804 + ybcM b0546 0.16 +0.1 570,804 + ybcM b0546 0.16 -0.7 570,804 + ybcM b0546 0.16 -0.8 570,804 + ybcM b0546 0.16 +1.0 570,804 + ybcM b0546 0.16 +2.0 570,817 + ybcM b0546 0.18 +0.0 570,896 + ybcM b0546 0.27 +1.2 570,992 + ybcM b0546 0.39 -0.4 570,992 + ybcM b0546 0.39 +1.3 571,055 + ybcM b0546 0.47 -1.8 571,084 - ybcM b0546 0.51 +0.2 571,094 - ybcM b0546 0.52 +2.2 571,105 + ybcM b0546 0.54 +1.2 571,115 + ybcM b0546 0.55 +1.2 571,124 - ybcM b0546 0.56 +1.2 571,130 - ybcM b0546 0.57 +1.2 571,170 - ybcM b0546 0.62 +1.2 571,186 + ybcM b0546 0.64 +2.7 571,207 + ybcM b0546 0.66 -1.0 571,207 + ybcM b0546 0.66 +1.2 571,215 - ybcM b0546 0.67 +1.2 571,226 + ybcM b0546 0.69 +1.3 571,283 + ybcM b0546 0.76 +0.5 571,301 + ybcM b0546 0.78 +0.8 571,301 + ybcM b0546 0.78 +0.4 571,306 + ybcM b0546 0.79 -0.7 571,340 + ybcM b0546 0.83 +1.2 571,340 + ybcM b0546 0.83 +1.2 571,340 + ybcM b0546 0.83 +0.4 571,340 + ybcM b0546 0.83 -0.6 571,341 - ybcM b0546 0.83 +1.2 571,345 - ybcM b0546 0.84 +2.2 571,358 - ybcM b0546 0.85 +1.2 571,418 + -0.7 571,427 + -0.4 571,477 - +0.2 571,486 - +1.7 571,546 - +0.3 571,560 - +0.8 571,575 - -1.2 571,575 - -0.1 571,585 - -0.5 571,596 + +0.7 571,596 - +0.7 571,596 - -0.2 571,602 - ylcH b4588 0.11 -0.0 571,618 + ylcH b4588 0.26 +1.2 571,623 - ylcH b4588 0.31 -0.9 571,629 + ylcH b4588 0.37 -1.2 571,638 - ylcH b4588 0.46 -0.1 571,671 + ylcH b4588 0.78 +0.8 571,681 + ylcH b4588 0.88 -1.7 571,681 + ylcH b4588 0.88 -1.8 571,682 - -0.7 571,682 - -0.3 571,682 - +1.5 571,682 - -0.4 571,682 - -0.4 571,682 - +0.3 571,682 - -0.6 571,687 + -0.4 571,694 - -0.4 571,695 - -0.4 571,752 + ybcN b0547 0.14 -0.3 571,752 + ybcN b0547 0.14 +0.0 571,909 + ybcN b0547 0.48 -0.7 571,931 + ybcN b0547 0.53 -0.1 571,937 - ybcN b0547 0.54 +0.0 571,939 - ybcN b0547 0.55 -0.9 571,939 - ybcN b0547 0.55 -0.2 572,055 + ybcN b0547 0.80 -0.6 572,068 - ybcN b0547 0.83 +0.8 572,179 + ninE b0548 0.20 -1.5 572,179 + ninE b0548 0.20 -0.2 572,179 + ninE b0548 0.20 -1.1 572,195 - ninE b0548 0.30 -0.2 572,214 - ninE b0548 0.41 +0.2 572,343 - ybcO b0549 0.12 -0.4
Or see this region's nucleotide sequence