Experiment: diversity test of Keio_ML9a_ucsf
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaC and hyaD overlap by 4 nucleotides hyaD and hyaE overlap by 4 nucleotides hyaE and hyaF overlap by 4 nucleotides hyaF and appC are separated by 133 nucleotides
b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996
hyaC
b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580
hyaD
b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975
hyaE
b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829
hyaF
b0978: appC - cytochrome bd-II oxidase, subunit I (NCBI), at 1,036,963 to 1,038,507
appC
Position (kb)
1035
1036
1037 Strain fitness (log2 ratio)
-1
0
1 at 1035.102 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD at 1035.291 kb on - strand, within hyaD at 1035.291 kb on - strand, within hyaD at 1035.310 kb on + strand, within hyaD at 1035.437 kb on + strand, within hyaD at 1035.437 kb on + strand, within hyaD at 1035.495 kb on + strand, within hyaD at 1035.503 kb on - strand, within hyaD at 1035.503 kb on - strand, within hyaD at 1035.803 kb on + strand, within hyaE at 1035.813 kb on + strand, within hyaE at 1035.892 kb on + strand, within hyaE at 1035.910 kb on - strand, within hyaE at 1035.910 kb on - strand, within hyaE at 1035.990 kb on + strand at 1036.062 kb on - strand, within hyaF at 1036.216 kb on + strand, within hyaF at 1036.216 kb on + strand, within hyaF at 1036.275 kb on + strand, within hyaF at 1036.294 kb on + strand, within hyaF at 1036.394 kb on - strand, within hyaF at 1036.463 kb on + strand at 1036.690 kb on - strand, within hyaF at 1036.690 kb on - strand, within hyaF at 1036.774 kb on - strand at 1036.791 kb on - strand at 1036.863 kb on - strand at 1036.863 kb on - strand at 1037.041 kb on - strand at 1037.175 kb on + strand, within appC at 1037.240 kb on - strand, within appC at 1037.240 kb on - strand, within appC at 1037.262 kb on + strand, within appC at 1037.266 kb on + strand, within appC at 1037.367 kb on - strand, within appC at 1037.378 kb on + strand, within appC at 1037.396 kb on - strand, within appC at 1037.766 kb on + strand, within appC
Per-strain Table
Position Strand Gene LocusTag Fraction diversity test of Keio_ML9a_ucsf remove 1,035,102 + hyaD b0975 0.19 -0.9 1,035,201 + hyaD b0975 0.35 -0.3 1,035,201 + hyaD b0975 0.35 +1.1 1,035,291 - hyaD b0975 0.51 +1.0 1,035,291 - hyaD b0975 0.51 -0.9 1,035,310 + hyaD b0975 0.54 +0.1 1,035,437 + hyaD b0975 0.76 -0.7 1,035,437 + hyaD b0975 0.76 -0.0 1,035,495 + hyaD b0975 0.85 +0.4 1,035,503 - hyaD b0975 0.87 -0.9 1,035,503 - hyaD b0975 0.87 -0.5 1,035,803 + hyaE b0976 0.57 +0.0 1,035,813 + hyaE b0976 0.59 +0.2 1,035,892 + hyaE b0976 0.79 +0.2 1,035,910 - hyaE b0976 0.83 +0.6 1,035,910 - hyaE b0976 0.83 -0.1 1,035,990 + +0.1 1,036,062 - hyaF b0977 0.10 -0.2 1,036,216 + hyaF b0977 0.28 -0.4 1,036,216 + hyaF b0977 0.28 -0.4 1,036,275 + hyaF b0977 0.35 +0.0 1,036,294 + hyaF b0977 0.38 -1.0 1,036,394 - hyaF b0977 0.49 -0.2 1,036,463 + +1.7 1,036,690 - hyaF b0977 0.84 -0.2 1,036,690 - hyaF b0977 0.84 -0.3 1,036,774 - -0.1 1,036,791 - +0.2 1,036,863 - +0.1 1,036,863 - -0.9 1,037,041 - -0.7 1,037,175 + appC b0978 0.14 +0.3 1,037,240 - appC b0978 0.18 +0.6 1,037,240 - appC b0978 0.18 +0.7 1,037,262 + appC b0978 0.19 -0.3 1,037,266 + appC b0978 0.20 +0.1 1,037,367 - appC b0978 0.26 -0.3 1,037,378 + appC b0978 0.27 +0.7 1,037,396 - appC b0978 0.28 -0.1 1,037,766 + appC b0978 0.52 -0.5
Or see this region's nucleotide sequence