Strain Fitness in Escherichia coli BW25113 around b0453

Experiment: diversity test of Keio_ML9a_ucsf

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttesB and ybaY are separated by 217 nucleotidesybaY and ybaZ are separated by 30 nucleotidesybaZ and ffs are separated by 76 nucleotidesffs and ybaA are separated by 110 nucleotides b0452: tesB - acyl-CoA thioesterase II (NCBI), at 473,525 to 474,385 tesB b0453: ybaY - glycoprotein/polysaccharide metabolism (VIMSS), at 474,603 to 475,175 ybaY b0454: ybaZ - predicted methyltransferase (NCBI), at 475,206 to 475,595 ybaZ b0455: ffs - 4.5S RNA (NCBI), at 475,672 to 475,785 ffs b0456: ybaA - hypothetical protein (NCBI), at 475,896 to 476,249 ybaA Position (kb) 474 475 476Strain fitness (log2 ratio) -1 0 1at 473.644 kb on - strand, within tesBat 473.651 kb on - strand, within tesBat 473.651 kb on - strand, within tesBat 473.651 kb on - strand, within tesBat 473.651 kb on - strand, within tesBat 473.855 kb on + strand, within tesBat 473.855 kb on + strand, within tesBat 473.901 kb on - strand, within tesBat 474.002 kb on + strand, within tesBat 474.055 kb on - strand, within tesBat 474.065 kb on - strand, within tesBat 474.105 kb on + strand, within tesBat 474.135 kb on - strand, within tesBat 474.135 kb on - strand, within tesBat 474.143 kb on - strand, within tesBat 474.145 kb on - strand, within tesBat 474.169 kb on + strand, within tesBat 474.195 kb on + strand, within tesBat 474.303 kb on - strandat 474.390 kb on + strandat 474.403 kb on - strandat 474.464 kb on + strandat 474.464 kb on + strandat 474.521 kb on - strandat 474.609 kb on + strandat 474.648 kb on - strandat 474.730 kb on + strand, within ybaYat 474.752 kb on + strand, within ybaYat 474.752 kb on + strand, within ybaYat 474.796 kb on + strand, within ybaYat 474.796 kb on + strand, within ybaYat 474.835 kb on + strand, within ybaYat 474.857 kb on + strand, within ybaYat 474.912 kb on + strand, within ybaYat 474.956 kb on - strand, within ybaYat 474.956 kb on - strand, within ybaYat 474.966 kb on - strand, within ybaYat 474.989 kb on + strand, within ybaYat 475.003 kb on - strand, within ybaYat 475.003 kb on - strand, within ybaYat 475.074 kb on + strand, within ybaYat 475.133 kb on + strandat 475.153 kb on + strandat 475.188 kb on - strandat 475.189 kb on + strandat 475.210 kb on - strandat 475.293 kb on + strand, within ybaZat 475.293 kb on - strand, within ybaZat 475.365 kb on - strand, within ybaZat 475.413 kb on - strand, within ybaZat 475.413 kb on - strand, within ybaZat 475.436 kb on - strand, within ybaZat 475.444 kb on - strand, within ybaZat 475.444 kb on - strand, within ybaZat 475.463 kb on - strand, within ybaZat 475.483 kb on - strand, within ybaZat 475.964 kb on + strand, within ybaAat 476.028 kb on - strand, within ybaAat 476.028 kb on - strand, within ybaAat 476.167 kb on + strand, within ybaA

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Per-strain Table

Position Strand Gene LocusTag Fraction diversity test of Keio_ML9a_ucsf
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473,644 - tesB b0452 0.14 -0.1
473,651 - tesB b0452 0.15 -0.1
473,651 - tesB b0452 0.15 +0.1
473,651 - tesB b0452 0.15 -0.0
473,651 - tesB b0452 0.15 -0.3
473,855 + tesB b0452 0.38 -1.3
473,855 + tesB b0452 0.38 +0.2
473,901 - tesB b0452 0.44 +0.2
474,002 + tesB b0452 0.55 -0.5
474,055 - tesB b0452 0.62 +0.2
474,065 - tesB b0452 0.63 -0.4
474,105 + tesB b0452 0.67 +0.2
474,135 - tesB b0452 0.71 -0.1
474,135 - tesB b0452 0.71 -0.1
474,143 - tesB b0452 0.72 +0.0
474,145 - tesB b0452 0.72 -0.3
474,169 + tesB b0452 0.75 -0.0
474,195 + tesB b0452 0.78 +0.5
474,303 - -0.4
474,390 + -0.7
474,403 - +0.1
474,464 + +0.2
474,464 + +1.0
474,521 - -0.3
474,609 + +0.2
474,648 - -0.1
474,730 + ybaY b0453 0.22 -0.1
474,752 + ybaY b0453 0.26 -0.2
474,752 + ybaY b0453 0.26 -0.3
474,796 + ybaY b0453 0.34 -0.4
474,796 + ybaY b0453 0.34 +0.1
474,835 + ybaY b0453 0.40 -1.0
474,857 + ybaY b0453 0.44 -0.1
474,912 + ybaY b0453 0.54 -0.9
474,956 - ybaY b0453 0.62 -0.2
474,956 - ybaY b0453 0.62 -0.6
474,966 - ybaY b0453 0.63 -0.1
474,989 + ybaY b0453 0.67 -0.4
475,003 - ybaY b0453 0.70 -0.2
475,003 - ybaY b0453 0.70 -0.7
475,074 + ybaY b0453 0.82 -0.7
475,133 + -0.4
475,153 + +0.1
475,188 - -0.7
475,189 + +0.3
475,210 - -0.1
475,293 + ybaZ b0454 0.22 -0.3
475,293 - ybaZ b0454 0.22 -0.7
475,365 - ybaZ b0454 0.41 -0.3
475,413 - ybaZ b0454 0.53 -0.8
475,413 - ybaZ b0454 0.53 +0.5
475,436 - ybaZ b0454 0.59 +0.1
475,444 - ybaZ b0454 0.61 +0.0
475,444 - ybaZ b0454 0.61 +0.2
475,463 - ybaZ b0454 0.66 -0.4
475,483 - ybaZ b0454 0.71 -0.6
475,964 + ybaA b0456 0.19 +0.4
476,028 - ybaA b0456 0.37 -0.6
476,028 - ybaA b0456 0.37 +0.3
476,167 + ybaA b0456 0.77 +0.4

Or see this region's nucleotide sequence