Strain Fitness in Escherichia coli BW25113 around b2019

Experiment: BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyoeB and yefM overlap by 4 nucleotidesyefM and hisL are separated by 282 nucleotideshisL and hisG are separated by 145 nucleotideshisG and hisD are separated by 5 nucleotides b4539: yoeB - toxin of the YoeB-YefM toxin-antitoxin system (NCBI), at 2,087,235 to 2,087,489 yoeB b2017: yefM - orf, hypothetical protein (VIMSS), at 2,087,486 to 2,087,737 yefM b2018: hisL - his operon leader peptide (NCBI), at 2,088,020 to 2,088,070 hisL b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115 hisG b2020: hisD - histidinol dehydrogenase (NCBI), at 2,089,121 to 2,090,425 hisD Position (kb) 2088 2089 2090Strain fitness (log2 ratio) -2 -1 0 1at 2087.364 kb on - strand, within yoeBat 2087.506 kb on - strandat 2087.506 kb on - strandat 2087.544 kb on + strand, within yefMat 2087.642 kb on - strand, within yefMat 2087.867 kb on + strandat 2088.113 kb on - strandat 2088.203 kb on - strandat 2088.203 kb on - strandat 2088.316 kb on + strand, within hisGat 2088.484 kb on - strand, within hisGat 2088.602 kb on + strand, within hisGat 2088.610 kb on - strand, within hisGat 2088.610 kb on - strand, within hisGat 2088.657 kb on - strand, within hisGat 2088.681 kb on - strand, within hisGat 2088.859 kb on + strand, within hisGat 2088.900 kb on + strand, within hisGat 2088.900 kb on + strand, within hisGat 2089.029 kb on + strandat 2089.104 kb on + strandat 2089.133 kb on - strandat 2089.155 kb on - strandat 2089.187 kb on - strandat 2089.234 kb on - strandat 2089.234 kb on - strandat 2089.267 kb on + strand, within hisDat 2089.326 kb on + strand, within hisDat 2089.419 kb on - strand, within hisDat 2089.504 kb on + strand, within hisDat 2089.605 kb on - strand, within hisDat 2089.755 kb on - strand, within hisDat 2089.776 kb on + strand, within hisDat 2089.844 kb on + strand, within hisDat 2089.929 kb on - strand, within hisDat 2089.929 kb on - strand, within hisDat 2090.018 kb on - strand, within hisDat 2090.018 kb on - strand, within hisDat 2090.075 kb on - strand, within hisD

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
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2,087,364 - yoeB b4539 0.51 -1.0
2,087,506 - -1.3
2,087,506 - -1.1
2,087,544 + yefM b2017 0.23 -0.8
2,087,642 - yefM b2017 0.62 -1.8
2,087,867 + +0.6
2,088,113 - +0.2
2,088,203 - -0.4
2,088,203 - +0.6
2,088,316 + hisG b2019 0.11 -0.0
2,088,484 - hisG b2019 0.30 +0.4
2,088,602 + hisG b2019 0.43 -0.5
2,088,610 - hisG b2019 0.44 +0.3
2,088,610 - hisG b2019 0.44 -0.1
2,088,657 - hisG b2019 0.49 +1.6
2,088,681 - hisG b2019 0.52 -0.2
2,088,859 + hisG b2019 0.71 -0.0
2,088,900 + hisG b2019 0.76 -1.3
2,088,900 + hisG b2019 0.76 +0.0
2,089,029 + +0.1
2,089,104 + -0.4
2,089,133 - -0.2
2,089,155 - +0.3
2,089,187 - -0.6
2,089,234 - -0.5
2,089,234 - +0.1
2,089,267 + hisD b2020 0.11 +0.3
2,089,326 + hisD b2020 0.16 +0.2
2,089,419 - hisD b2020 0.23 +0.2
2,089,504 + hisD b2020 0.29 +0.3
2,089,605 - hisD b2020 0.37 +0.1
2,089,755 - hisD b2020 0.49 +0.3
2,089,776 + hisD b2020 0.50 -0.1
2,089,844 + hisD b2020 0.55 -0.1
2,089,929 - hisD b2020 0.62 -0.1
2,089,929 - hisD b2020 0.62 -0.4
2,090,018 - hisD b2020 0.69 -0.4
2,090,018 - hisD b2020 0.69 -0.3
2,090,075 - hisD b2020 0.73 +0.5

Or see this region's nucleotide sequence