Strain Fitness in Escherichia coli BW25113 around b3825

Experiment: BHIS with Methotrexate 1.98 mM 24 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	BHIS with Methotrexate 1.98 mM 24 hr
4,006,208	+	rhtC	b3823	0.69	+0.1
4,006,244	+	rhtC	b3823	0.75	-0.1
4,006,244	+	rhtC	b3823	0.75	-0.2
4,006,275	-	rhtC	b3823	0.80	-1.1
4,006,304	+	rhtC	b3823	0.84	-0.0
4,006,323	-	rhtC	b3823	0.87	-0.2
4,006,331	+	rhtC	b3823	0.89	-0.3
4,006,420	-				+0.4
4,006,421	-				+0.2
4,006,443	-				-1.6
4,006,480	+				+0.5
4,006,480	+				+0.1
4,006,486	-				-0.1
4,006,489	-				-0.1
4,006,491	+				+0.4
4,006,501	+				-0.6
4,006,501	-				-1.2
4,006,501	-				-0.0
4,006,528	-	rhtB	b3824	0.11	-0.3
4,006,542	+	rhtB	b3824	0.13	-0.1
4,006,545	-	rhtB	b3824	0.13	-0.2
4,006,558	+	rhtB	b3824	0.15	-0.2
4,006,566	-	rhtB	b3824	0.17	-0.4
4,006,602	+	rhtB	b3824	0.23	+0.1
4,006,602	+	rhtB	b3824	0.23	-0.0
4,006,610	-	rhtB	b3824	0.24	-0.3
4,006,622	-	rhtB	b3824	0.26	+0.1
4,006,622	-	rhtB	b3824	0.26	-0.3
4,006,627	-	rhtB	b3824	0.27	-0.3
4,006,627	-	rhtB	b3824	0.27	+0.9
4,006,638	+	rhtB	b3824	0.28	-0.0
4,006,638	+	rhtB	b3824	0.28	-0.2
4,006,640	+	rhtB	b3824	0.29	+0.1
4,006,645	-	rhtB	b3824	0.29	+0.1
4,006,645	-	rhtB	b3824	0.29	-0.3
4,006,646	-	rhtB	b3824	0.30	+0.2
4,006,752	-	rhtB	b3824	0.47	-0.0
4,006,764	+	rhtB	b3824	0.49	-0.1
4,006,770	-	rhtB	b3824	0.50	-1.3
4,006,825	-	rhtB	b3824	0.58	-0.3
4,006,853	-	rhtB	b3824	0.63	+0.1
4,006,861	-	rhtB	b3824	0.64	-0.1
4,006,903	-	rhtB	b3824	0.71	+0.5
4,006,912	+	rhtB	b3824	0.72	+0.1
4,006,947	+	rhtB	b3824	0.78	-0.3
4,006,960	-	rhtB	b3824	0.80	-0.0
4,006,972	+	rhtB	b3824	0.82	-0.6
4,006,981	-	rhtB	b3824	0.84	-0.0
4,007,013	+	rhtB	b3824	0.89	-0.4
4,007,013	+	rhtB	b3824	0.89	+1.0
4,007,021	+				-0.0
4,007,056	-				-0.3
4,007,077	+				+0.3
4,007,077	+				+0.3
4,007,081	+				+0.0
4,007,096	-				-0.5
4,007,096	-				-0.8
4,007,115	+				+0.2
4,007,138	+				-0.2
4,007,138	+				-0.2
4,007,148	-				+0.0
4,007,148	-				-0.4
4,007,148	-				-0.5
4,007,202	-				-0.0
4,007,264	-				-0.3
4,007,264	-				-1.6
4,007,289	-				-1.0
4,007,289	-				-0.4
4,007,319	-	pldB	b3825	0.12	-1.0
4,007,345	+	pldB	b3825	0.15	+0.3
4,007,345	+	pldB	b3825	0.15	-0.7
4,007,354	-	pldB	b3825	0.16	-0.2
4,007,357	+	pldB	b3825	0.16	-0.4
4,007,357	+	pldB	b3825	0.16	-1.1
4,007,361	+	pldB	b3825	0.16	-1.3
4,007,364	-	pldB	b3825	0.17	-0.2
4,007,366	+	pldB	b3825	0.17	-0.8
4,007,379	-	pldB	b3825	0.18	-0.9
4,007,380	+	pldB	b3825	0.18	-1.4
4,007,382	+	pldB	b3825	0.18	-0.6
4,007,411	+	pldB	b3825	0.21	-0.8
4,007,421	-	pldB	b3825	0.22	-0.8
4,007,421	-	pldB	b3825	0.22	-1.1
4,007,483	+	pldB	b3825	0.28	-0.7
4,007,483	+	pldB	b3825	0.28	-0.5
4,007,491	-	pldB	b3825	0.29	-0.3
4,007,518	-	pldB	b3825	0.32	-1.3
4,007,526	-	pldB	b3825	0.33	+0.2
4,007,559	+	pldB	b3825	0.36	-0.0
4,007,559	+	pldB	b3825	0.36	-0.8
4,007,564	+	pldB	b3825	0.36	-0.9
4,007,565	+	pldB	b3825	0.36	-0.6
4,007,590	+	pldB	b3825	0.39	-0.9
4,007,614	-	pldB	b3825	0.41	-0.2
4,007,652	+	pldB	b3825	0.45	-0.9
4,007,667	+	pldB	b3825	0.46	-0.8
4,007,667	+	pldB	b3825	0.46	-0.5
4,007,731	+	pldB	b3825	0.53	-1.4
4,007,731	+	pldB	b3825	0.53	-0.5
4,007,749	-	pldB	b3825	0.54	-1.6
4,007,749	-	pldB	b3825	0.54	-0.2
4,007,811	+	pldB	b3825	0.60	-0.6
4,007,811	+	pldB	b3825	0.60	-0.2
4,007,861	+	pldB	b3825	0.65	-0.9
4,007,869	-	pldB	b3825	0.66	-0.1
4,007,869	-	pldB	b3825	0.66	-0.8
4,007,871	-	pldB	b3825	0.66	-0.4
4,007,875	+	pldB	b3825	0.67	-0.8
4,007,875	+	pldB	b3825	0.67	-0.5
4,007,881	-	pldB	b3825	0.67	-0.7
4,007,903	-	pldB	b3825	0.69	-0.6
4,008,002	-	pldB	b3825	0.79	-0.7
4,008,002	-	pldB	b3825	0.79	-0.7
4,008,017	+	pldB	b3825	0.81	-0.5
4,008,017	-	pldB	b3825	0.81	-0.5
4,008,025	-	pldB	b3825	0.81	-0.7
4,008,041	+	pldB	b3825	0.83	-1.2
4,008,054	-	pldB	b3825	0.84	-1.1
4,008,060	-	pldB	b3825	0.85	-0.8
4,008,066	+	pldB	b3825	0.85	-1.2
4,008,086	+	pldB	b3825	0.87	-2.2
4,008,086	+	pldB	b3825	0.87	-1.4
4,008,225	+				-0.1
4,008,230	+				+0.1
4,008,230	+				-0.2
4,008,302	+				+0.1
4,008,302	+				+0.3
4,008,310	-	yigL	b3826	0.11	-0.7
4,008,350	+	yigL	b3826	0.16	+0.1
4,008,350	+	yigL	b3826	0.16	-0.1
4,008,393	-	yigL	b3826	0.21	-0.2
4,008,416	+	yigL	b3826	0.24	+0.1
4,008,430	+	yigL	b3826	0.26	-0.4
4,008,489	-	yigL	b3826	0.33	-0.2
4,008,495	+	yigL	b3826	0.34	+0.2
4,008,496	-	yigL	b3826	0.34	-0.7
4,008,505	+	yigL	b3826	0.35	+0.0
4,008,526	-	yigL	b3826	0.38	-1.6
4,008,526	-	yigL	b3826	0.38	+0.1
4,008,529	+	yigL	b3826	0.38	+0.0
4,008,572	+	yigL	b3826	0.44	+0.7
4,008,572	+	yigL	b3826	0.44	-0.3
4,008,621	+	yigL	b3826	0.50	-0.5
4,008,629	-	yigL	b3826	0.51	+0.1
4,008,629	-	yigL	b3826	0.51	-0.2
4,008,629	-	yigL	b3826	0.51	-0.0
4,008,632	+	yigL	b3826	0.51	+0.3
4,008,677	+	yigL	b3826	0.57	+0.4
4,008,677	+	yigL	b3826	0.57	+0.0
4,008,680	+	yigL	b3826	0.57	+0.3
4,008,688	-	yigL	b3826	0.58	-0.2
4,008,708	+	yigL	b3826	0.61	+0.3
4,008,754	+	yigL	b3826	0.66	-0.2
4,008,754	+	yigL	b3826	0.66	+0.4
4,008,754	+	yigL	b3826	0.66	+0.4
4,008,754	+	yigL	b3826	0.66	-0.4
4,008,762	-	yigL	b3826	0.67	+0.8
4,008,762	-	yigL	b3826	0.67	-0.3
4,008,903	-	yigL	b3826	0.85	+0.8
4,008,910	+	yigL	b3826	0.86	+0.1
4,008,918	-	yigL	b3826	0.87	+0.2
4,008,920	+	yigL	b3826	0.87	+0.0
4,008,920	+	yigL	b3826	0.87	+0.2
4,008,920	+	yigL	b3826	0.87	-0.0
4,008,968	+				-0.1
4,009,067	-				-0.3
4,009,082	-				-0.2
4,009,134	-				-0.0
4,009,176	-				+0.5
4,009,176	-				+0.1
4,009,205	-	yigM	b3827	0.12	-0.6