Experiment: BHIS with Methotrexate 1.98 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt map and tff are separated by 205 nucleotides tff and rpsB are separated by 26 nucleotides
b0168: map - methionine aminopeptidase (NCBI), at 188,712 to 189,506
map
b4414: tff - unknown RNA (NCBI), at 189,712 to 189,847
tff
b0169: rpsB - 30S ribosomal protein S2 (NCBI), at 189,874 to 190,599
rpsB
Position (kb) 189
190 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 188.717 kb on - strand at 188.751 kb on - strand at 189.542 kb on + strand at 189.542 kb on + strand at 189.548 kb on - strand at 189.572 kb on - strand at 189.573 kb on - strand at 189.580 kb on + strand at 189.580 kb on + strand at 189.614 kb on + strand at 189.614 kb on + strand at 189.651 kb on + strand at 189.653 kb on + strand at 189.653 kb on + strand at 189.653 kb on + strand at 189.653 kb on + strand at 189.653 kb on + strand at 189.653 kb on + strand at 189.653 kb on + strand at 189.653 kb on + strand at 189.661 kb on - strand at 189.661 kb on - strand at 189.661 kb on - strand at 189.740 kb on + strand, within tff at 189.740 kb on + strand, within tff at 189.740 kb on + strand, within tff at 189.744 kb on + strand, within tff at 189.758 kb on + strand, within tff at 190.524 kb on + strand, within rpsB at 190.524 kb on + strand, within rpsB at 190.524 kb on + strand, within rpsB at 190.594 kb on + strand at 190.605 kb on + strand at 190.605 kb on + strand at 190.605 kb on + strand at 190.605 kb on + strand at 190.689 kb on + strand at 190.744 kb on + strand at 190.769 kb on + strand at 190.777 kb on + strand at 190.778 kb on + strand at 190.778 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 24 hr remove 188,717 - +0.1 188,751 - -0.8 189,542 + -1.6 189,542 + -2.3 189,548 - +0.0 189,572 - +0.0 189,573 - -0.0 189,580 + -0.3 189,580 + -1.3 189,614 + -1.1 189,614 + -0.6 189,651 + +0.1 189,653 + +0.2 189,653 + -0.9 189,653 + +0.1 189,653 + -0.3 189,653 + -1.1 189,653 + -0.2 189,653 + -1.1 189,653 + +0.1 189,661 - +0.4 189,661 - -0.1 189,661 - -0.2 189,740 + tff b4414 0.21 +0.6 189,740 + tff b4414 0.21 -2.9 189,740 + tff b4414 0.21 -2.0 189,744 + tff b4414 0.24 -2.0 189,758 + tff b4414 0.34 -1.9 190,524 + rpsB b0169 0.90 -1.0 190,524 + rpsB b0169 0.90 -2.0 190,524 + rpsB b0169 0.90 -1.4 190,594 + +1.0 190,605 + -0.4 190,605 + -0.7 190,605 + -1.3 190,605 + -1.6 190,689 + -0.1 190,744 + -0.1 190,769 + +1.0 190,777 + -0.4 190,778 + -0.3 190,778 + -0.5
Or see this region's nucleotide sequence