Strain Fitness in Escherichia coli BW25113 around b1325

Experiment: BHIS with Methotrexate 1.98 mM 24 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	BHIS with Methotrexate 1.98 mM 24 hr
1,385,987	-	tyrR	b1323	0.81	+0.3
1,386,014	+	tyrR	b1323	0.82	+0.1
1,386,352	-				-0.2
1,386,352	-				-0.8
1,386,442	+	tpx	b1324	0.22	+0.1
1,386,528	+	tpx	b1324	0.39	-1.1
1,386,585	+				-1.3
1,386,585	+	tpx	b1324	0.50	+1.5
1,386,603	-	tpx	b1324	0.54	+1.2
1,386,603	-	tpx	b1324	0.54	+0.5
1,386,742	-	tpx	b1324	0.81	-0.2
1,386,748	+	tpx	b1324	0.83	+0.6
1,386,923	+				-0.6
1,387,059	+	ycjG	b1325	0.11	+2.2
1,387,076	-	ycjG	b1325	0.13	-0.3
1,387,315	-				-1.5
1,387,352	+	ycjG	b1325	0.41	+0.0
1,387,360	-	ycjG	b1325	0.42	+0.1
1,387,417	-	ycjG	b1325	0.48	+1.1
1,387,417	-	ycjG	b1325	0.48	+0.0
1,387,485	+	ycjG	b1325	0.55	+0.0
1,387,837	-				+0.2
1,387,837	-				-0.1
1,388,090	+	mpaA	b1326	0.27	-0.2
1,388,195	-	mpaA	b1326	0.41	+0.4
1,388,195	-	mpaA	b1326	0.41	-0.4
1,388,195	-	mpaA	b1326	0.41	-0.0
1,388,351	-	mpaA	b1326	0.63	+0.0
1,388,438	+	mpaA	b1326	0.75	-0.1
1,388,438	+	mpaA	b1326	0.75	+0.1
1,388,446	-	mpaA	b1326	0.76	-0.1
1,388,611	+				-0.6
1,388,628	+				-0.2
1,388,645	+				-0.4
1,388,797	+	ymjC	b4525	0.35	-0.2