Experiment: BHIS with Methotrexate 1.98 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and sucD overlap by 1 nucleotides sucD and mngR are separated by 103 nucleotides mngR and mngA are separated by 108 nucleotides
b0728: sucC - succinyl-CoA synthetase subunit beta (NCBI), at 762,237 to 763,403
sucC
b0729: sucD - succinyl-CoA synthetase subunit alpha (NCBI), at 763,403 to 764,272
sucD
b0730: mngR - DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI), at 764,376 to 765,098
mngR
b0731: mngA - fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component (NCBI), at 765,207 to 767,183
mngA
Position (kb) 763
764
765 Strain fitness (log2 ratio) -1
0
1
2
3 at 762.600 kb on - strand, within sucC at 762.708 kb on + strand, within sucC at 762.708 kb on + strand, within sucC at 762.713 kb on + strand, within sucC at 762.989 kb on - strand, within sucC at 762.999 kb on - strand, within sucC at 763.015 kb on + strand, within sucC at 763.070 kb on - strand, within sucC at 763.131 kb on + strand, within sucC at 763.206 kb on - strand, within sucC at 763.206 kb on - strand, within sucC at 763.206 kb on - strand, within sucC at 763.278 kb on + strand, within sucC at 763.625 kb on + strand, within sucD at 763.625 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.773 kb on + strand, within sucD at 763.871 kb on - strand, within sucD at 763.901 kb on + strand, within sucD at 764.252 kb on - strand at 764.279 kb on + strand at 764.302 kb on + strand at 764.302 kb on + strand at 764.311 kb on + strand at 764.319 kb on + strand at 764.349 kb on + strand at 764.480 kb on + strand, within mngR at 764.480 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.645 kb on + strand, within mngR at 764.668 kb on + strand, within mngR at 764.811 kb on + strand, within mngR
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 24 hr remove 762,600 - sucC b0728 0.31 -0.5 762,708 + sucC b0728 0.40 +0.3 762,708 + sucC b0728 0.40 -0.5 762,713 + sucC b0728 0.41 -0.4 762,989 - sucC b0728 0.64 +0.4 762,999 - sucC b0728 0.65 +0.1 763,015 + sucC b0728 0.67 -0.1 763,070 - sucC b0728 0.71 +0.4 763,131 + sucC b0728 0.77 -0.8 763,206 - sucC b0728 0.83 -0.2 763,206 - sucC b0728 0.83 -0.1 763,206 - sucC b0728 0.83 -0.3 763,278 + sucC b0728 0.89 +0.0 763,625 + sucD b0729 0.26 -1.3 763,625 + sucD b0729 0.26 +0.5 763,712 + sucD b0729 0.36 -1.5 763,712 + sucD b0729 0.36 -0.5 763,773 + sucD b0729 0.43 -0.8 763,871 - sucD b0729 0.54 -0.8 763,901 + sucD b0729 0.57 -1.3 764,252 - +0.0 764,279 + -0.3 764,302 + +0.1 764,302 + -0.7 764,311 + -0.8 764,319 + -0.3 764,349 + +0.2 764,480 + mngR b0730 0.14 +0.2 764,480 + mngR b0730 0.14 -1.1 764,515 + mngR b0730 0.19 -0.1 764,515 + mngR b0730 0.19 -0.8 764,515 + mngR b0730 0.19 +1.2 764,515 + mngR b0730 0.19 -0.8 764,645 + mngR b0730 0.37 +0.9 764,668 + mngR b0730 0.40 +0.5 764,811 + mngR b0730 0.60 +2.8
Or see this region's nucleotide sequence