Experiment: BHIS with Methotrexate 1.98 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lipB and ybeD are separated by 100 nucleotides ybeD and dacA are separated by 109 nucleotides dacA and rlpA are separated by 138 nucleotides
b0630: lipB - protein of lipoate biosynthesis (VIMSS), at 660,860 to 661,501
lipB
b0631: ybeD - hypothetical protein (NCBI), at 661,602 to 661,865
ybeD
b0632: dacA - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI), at 661,975 to 663,186
dacA
b0633: rlpA - minor lipoprotein (NCBI), at 663,325 to 664,413
rlpA
Position (kb) 661
662
663
664 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 661.084 kb on - strand, within lipB at 661.146 kb on + strand, within lipB at 661.264 kb on + strand, within lipB at 661.272 kb on - strand, within lipB at 661.509 kb on + strand at 661.519 kb on - strand at 661.957 kb on - strand at 661.957 kb on - strand at 662.131 kb on + strand, within dacA at 662.153 kb on - strand, within dacA at 662.155 kb on + strand, within dacA at 662.160 kb on + strand, within dacA at 662.160 kb on + strand, within dacA at 662.255 kb on + strand, within dacA at 662.380 kb on - strand, within dacA at 662.380 kb on - strand, within dacA at 662.462 kb on - strand, within dacA at 662.462 kb on - strand, within dacA at 662.609 kb on - strand, within dacA at 662.609 kb on - strand, within dacA at 662.618 kb on + strand, within dacA at 662.618 kb on + strand, within dacA at 662.618 kb on + strand, within dacA at 662.618 kb on + strand, within dacA at 662.618 kb on + strand, within dacA at 662.620 kb on + strand, within dacA at 662.644 kb on + strand, within dacA at 662.644 kb on + strand, within dacA at 662.644 kb on + strand, within dacA at 662.644 kb on + strand, within dacA at 662.652 kb on - strand, within dacA at 662.652 kb on - strand, within dacA at 662.864 kb on + strand, within dacA at 662.867 kb on - strand, within dacA at 662.867 kb on - strand, within dacA at 662.889 kb on - strand, within dacA at 662.889 kb on - strand, within dacA at 663.038 kb on + strand, within dacA at 663.053 kb on - strand, within dacA at 663.060 kb on + strand, within dacA at 663.096 kb on - strand at 663.096 kb on - strand at 663.123 kb on - strand at 663.140 kb on - strand at 663.147 kb on - strand at 663.147 kb on - strand at 663.165 kb on - strand at 663.236 kb on - strand at 663.236 kb on - strand at 663.325 kb on - strand at 663.391 kb on + strand at 663.391 kb on + strand at 663.394 kb on - strand at 663.427 kb on - strand at 663.465 kb on - strand, within rlpA at 663.465 kb on - strand, within rlpA at 663.475 kb on - strand, within rlpA at 663.532 kb on - strand, within rlpA at 663.581 kb on + strand, within rlpA at 663.581 kb on + strand, within rlpA at 663.620 kb on + strand, within rlpA at 663.697 kb on - strand, within rlpA at 663.770 kb on - strand, within rlpA at 663.775 kb on - strand, within rlpA at 663.775 kb on - strand, within rlpA at 663.844 kb on - strand, within rlpA at 663.849 kb on - strand, within rlpA at 663.849 kb on - strand, within rlpA at 663.860 kb on + strand, within rlpA at 663.865 kb on + strand, within rlpA at 663.865 kb on + strand, within rlpA at 663.867 kb on + strand, within rlpA at 663.873 kb on - strand, within rlpA at 663.894 kb on + strand, within rlpA at 663.900 kb on - strand, within rlpA at 663.900 kb on - strand, within rlpA at 663.941 kb on - strand, within rlpA at 664.174 kb on + strand, within rlpA
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 24 hr remove 661,084 - lipB b0630 0.35 +0.2 661,146 + lipB b0630 0.45 +1.2 661,264 + lipB b0630 0.63 -0.4 661,272 - lipB b0630 0.64 -1.4 661,509 + -0.4 661,519 - -0.1 661,957 - -0.4 661,957 - +0.2 662,131 + dacA b0632 0.13 -0.4 662,153 - dacA b0632 0.15 -1.8 662,155 + dacA b0632 0.15 +1.2 662,160 + dacA b0632 0.15 -0.4 662,160 + dacA b0632 0.15 +0.6 662,255 + dacA b0632 0.23 -2.2 662,380 - dacA b0632 0.33 -1.6 662,380 - dacA b0632 0.33 -2.0 662,462 - dacA b0632 0.40 -2.6 662,462 - dacA b0632 0.40 -2.5 662,609 - dacA b0632 0.52 -2.7 662,609 - dacA b0632 0.52 -1.7 662,618 + dacA b0632 0.53 -2.5 662,618 + dacA b0632 0.53 -2.7 662,618 + dacA b0632 0.53 -2.1 662,618 + dacA b0632 0.53 -2.9 662,618 + dacA b0632 0.53 -1.2 662,620 + dacA b0632 0.53 -2.2 662,644 + dacA b0632 0.55 -1.1 662,644 + dacA b0632 0.55 -1.4 662,644 + dacA b0632 0.55 +0.6 662,644 + dacA b0632 0.55 -0.4 662,652 - dacA b0632 0.56 -2.4 662,652 - dacA b0632 0.56 -1.2 662,864 + dacA b0632 0.73 -2.4 662,867 - dacA b0632 0.74 -1.2 662,867 - dacA b0632 0.74 -2.0 662,889 - dacA b0632 0.75 -2.1 662,889 - dacA b0632 0.75 -1.4 663,038 + dacA b0632 0.88 -4.0 663,053 - dacA b0632 0.89 -1.3 663,060 + dacA b0632 0.90 -0.8 663,096 - -1.7 663,096 - -2.7 663,123 - -2.2 663,140 - -1.5 663,147 - -2.8 663,147 - -0.7 663,165 - +0.3 663,236 - +0.3 663,236 - -0.9 663,325 - +0.4 663,391 + -0.4 663,391 + +0.1 663,394 - -1.3 663,427 - +0.2 663,465 - rlpA b0633 0.13 +0.8 663,465 - rlpA b0633 0.13 +0.2 663,475 - rlpA b0633 0.14 -0.3 663,532 - rlpA b0633 0.19 -0.5 663,581 + rlpA b0633 0.24 +0.0 663,581 + rlpA b0633 0.24 +0.5 663,620 + rlpA b0633 0.27 -0.3 663,697 - rlpA b0633 0.34 +0.1 663,770 - rlpA b0633 0.41 -0.7 663,775 - rlpA b0633 0.41 +0.2 663,775 - rlpA b0633 0.41 +0.3 663,844 - rlpA b0633 0.48 -0.2 663,849 - rlpA b0633 0.48 +0.4 663,849 - rlpA b0633 0.48 -0.0 663,860 + rlpA b0633 0.49 -0.3 663,865 + rlpA b0633 0.50 -0.3 663,865 + rlpA b0633 0.50 -1.5 663,867 + rlpA b0633 0.50 -0.8 663,873 - rlpA b0633 0.50 -0.5 663,894 + rlpA b0633 0.52 -0.1 663,900 - rlpA b0633 0.53 -0.7 663,900 - rlpA b0633 0.53 -0.3 663,941 - rlpA b0633 0.57 -0.1 664,174 + rlpA b0633 0.78 -0.4
Or see this region's nucleotide sequence