Experiment: BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiC and yifN are separated by 198 nucleotides yifN and rep are separated by 216 nucleotides rep and gpp are separated by 46 nucleotides
b3775: ppiC - peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI), at 3,957,555 to 3,957,836
ppiC
b3777: yifN - conserved protein (pseudogene) (NCBI), at 3,958,035 to 3,958,483
yifN
b3778: rep - DNA helicase and single-stranded DNA-dependent ATPase (NCBI), at 3,958,700 to 3,960,721
rep
b3779: gpp - guanosine pentaphosphatase/exopolyphosphatase (NCBI), at 3,960,768 to 3,962,252
gpp
Position (kb)
3958
3959
3960
3961 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3957.765 kb on - strand, within ppiC at 3957.767 kb on + strand, within ppiC at 3957.767 kb on + strand, within ppiC at 3957.838 kb on + strand at 3957.838 kb on + strand at 3957.842 kb on + strand at 3957.842 kb on + strand at 3957.842 kb on + strand at 3957.916 kb on - strand at 3957.916 kb on - strand at 3957.922 kb on + strand at 3957.931 kb on - strand at 3957.966 kb on - strand at 3957.966 kb on - strand at 3957.999 kb on - strand at 3958.000 kb on + strand at 3958.006 kb on - strand at 3958.053 kb on + strand at 3958.053 kb on + strand at 3958.073 kb on - strand at 3958.080 kb on + strand, within yifN at 3958.082 kb on + strand, within yifN at 3958.088 kb on - strand, within yifN at 3958.094 kb on - strand, within yifN at 3958.106 kb on - strand, within yifN at 3958.106 kb on - strand, within yifN at 3958.111 kb on + strand, within yifN at 3958.113 kb on - strand, within yifN at 3958.113 kb on - strand, within yifN at 3958.115 kb on + strand, within yifN at 3958.126 kb on + strand, within yifN at 3958.135 kb on - strand, within yifN at 3958.135 kb on - strand, within yifN at 3958.137 kb on - strand, within yifN at 3958.141 kb on + strand, within yifN at 3958.144 kb on - strand, within yifN at 3958.154 kb on - strand, within yifN at 3958.177 kb on - strand, within yifN at 3958.185 kb on - strand, within yifN at 3958.191 kb on - strand, within yifN at 3958.191 kb on - strand, within yifN at 3958.295 kb on - strand, within yifN at 3958.307 kb on - strand, within yifN at 3958.352 kb on + strand, within yifN at 3958.391 kb on - strand, within yifN at 3958.391 kb on - strand, within yifN at 3958.393 kb on - strand, within yifN at 3958.403 kb on - strand, within yifN at 3958.403 kb on - strand, within yifN at 3958.419 kb on + strand, within yifN at 3958.435 kb on - strand, within yifN at 3958.435 kb on - strand, within yifN at 3958.459 kb on + strand at 3958.483 kb on + strand at 3958.529 kb on + strand at 3958.659 kb on + strand at 3958.659 kb on + strand at 3958.705 kb on + strand at 3958.904 kb on - strand, within rep at 3958.904 kb on - strand, within rep at 3958.931 kb on + strand, within rep at 3958.942 kb on + strand, within rep at 3958.950 kb on - strand, within rep at 3958.961 kb on + strand, within rep at 3958.966 kb on + strand, within rep at 3958.970 kb on - strand, within rep at 3958.974 kb on - strand, within rep at 3959.079 kb on - strand, within rep at 3959.079 kb on - strand, within rep at 3959.093 kb on - strand, within rep at 3959.113 kb on - strand, within rep at 3959.195 kb on + strand, within rep at 3959.207 kb on - strand, within rep at 3959.207 kb on - strand, within rep at 3959.214 kb on - strand, within rep at 3959.215 kb on - strand, within rep at 3959.221 kb on - strand, within rep at 3959.222 kb on - strand, within rep at 3959.222 kb on - strand, within rep at 3959.229 kb on + strand, within rep at 3959.281 kb on - strand, within rep at 3959.351 kb on + strand, within rep at 3959.351 kb on + strand, within rep at 3959.352 kb on - strand, within rep at 3959.352 kb on - strand, within rep at 3959.361 kb on - strand, within rep at 3959.361 kb on - strand, within rep at 3959.371 kb on - strand, within rep at 3959.375 kb on + strand, within rep at 3959.376 kb on - strand, within rep at 3959.409 kb on + strand, within rep at 3959.414 kb on - strand, within rep at 3959.419 kb on + strand, within rep at 3959.454 kb on + strand, within rep at 3959.454 kb on + strand, within rep at 3959.460 kb on - strand at 3959.460 kb on - strand, within rep at 3959.509 kb on + strand, within rep at 3959.514 kb on + strand, within rep at 3959.524 kb on + strand, within rep at 3959.527 kb on - strand, within rep at 3959.541 kb on + strand, within rep at 3959.588 kb on - strand, within rep at 3959.597 kb on - strand, within rep at 3959.612 kb on + strand, within rep at 3959.625 kb on - strand, within rep at 3959.625 kb on - strand, within rep at 3959.729 kb on + strand, within rep at 3959.786 kb on + strand, within rep at 3959.865 kb on + strand, within rep at 3959.886 kb on - strand, within rep at 3959.886 kb on - strand, within rep at 3959.891 kb on - strand, within rep at 3959.940 kb on + strand, within rep at 3960.053 kb on + strand, within rep at 3960.053 kb on + strand, within rep at 3960.056 kb on - strand, within rep at 3960.056 kb on - strand, within rep at 3960.076 kb on - strand, within rep at 3960.154 kb on + strand, within rep at 3960.154 kb on + strand, within rep at 3960.243 kb on + strand at 3960.276 kb on - strand, within rep at 3960.290 kb on + strand, within rep at 3960.358 kb on + strand, within rep at 3960.366 kb on + strand, within rep at 3960.422 kb on - strand, within rep at 3960.465 kb on - strand, within rep at 3960.519 kb on - strand at 3960.530 kb on - strand at 3960.530 kb on - strand at 3960.532 kb on + strand at 3960.532 kb on + strand at 3960.532 kb on + strand at 3960.575 kb on - strand at 3960.740 kb on + strand at 3960.799 kb on - strand at 3960.837 kb on - strand at 3960.867 kb on - strand at 3960.889 kb on + strand at 3960.895 kb on + strand at 3960.895 kb on + strand at 3960.896 kb on + strand at 3960.975 kb on - strand, within gpp at 3961.019 kb on + strand, within gpp at 3961.038 kb on + strand, within gpp at 3961.042 kb on - strand, within gpp at 3961.052 kb on + strand, within gpp at 3961.052 kb on + strand, within gpp at 3961.052 kb on + strand, within gpp at 3961.055 kb on - strand, within gpp at 3961.055 kb on - strand, within gpp at 3961.115 kb on + strand, within gpp at 3961.131 kb on - strand, within gpp at 3961.151 kb on + strand, within gpp at 3961.277 kb on + strand, within gpp at 3961.299 kb on + strand, within gpp at 3961.314 kb on - strand, within gpp at 3961.324 kb on - strand, within gpp at 3961.324 kb on - strand, within gpp at 3961.324 kb on - strand, within gpp at 3961.326 kb on - strand, within gpp at 3961.327 kb on + strand, within gpp at 3961.360 kb on + strand, within gpp at 3961.360 kb on + strand, within gpp at 3961.365 kb on + strand, within gpp at 3961.365 kb on + strand, within gpp at 3961.382 kb on + strand, within gpp at 3961.405 kb on - strand, within gpp at 3961.407 kb on - strand, within gpp at 3961.407 kb on - strand, within gpp at 3961.407 kb on - strand, within gpp at 3961.451 kb on - strand, within gpp at 3961.451 kb on - strand, within gpp at 3961.453 kb on + strand, within gpp at 3961.473 kb on + strand, within gpp at 3961.473 kb on + strand, within gpp at 3961.481 kb on - strand, within gpp at 3961.481 kb on - strand, within gpp at 3961.501 kb on + strand, within gpp at 3961.501 kb on + strand, within gpp at 3961.523 kb on - strand, within gpp at 3961.526 kb on - strand, within gpp at 3961.532 kb on + strand, within gpp at 3961.566 kb on - strand, within gpp at 3961.571 kb on - strand, within gpp at 3961.605 kb on - strand, within gpp at 3961.605 kb on - strand, within gpp at 3961.615 kb on - strand, within gpp at 3961.615 kb on - strand, within gpp at 3961.620 kb on - strand, within gpp at 3961.621 kb on - strand, within gpp at 3961.671 kb on + strand, within gpp
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr remove 3,957,765 - ppiC b3775 0.74 -0.0 3,957,767 + ppiC b3775 0.75 -0.3 3,957,767 + ppiC b3775 0.75 -0.4 3,957,838 + -0.8 3,957,838 + -0.1 3,957,842 + +0.2 3,957,842 + -0.1 3,957,842 + -0.2 3,957,916 - +1.3 3,957,916 - +0.4 3,957,922 + -0.4 3,957,931 - -0.1 3,957,966 - -0.1 3,957,966 - -0.2 3,957,999 - -0.6 3,958,000 + +0.1 3,958,006 - -0.1 3,958,053 + -0.1 3,958,053 + -0.1 3,958,073 - +0.1 3,958,080 + yifN b3777 0.10 -0.2 3,958,082 + yifN b3777 0.10 +0.3 3,958,088 - yifN b3777 0.12 +0.2 3,958,094 - yifN b3777 0.13 -0.5 3,958,106 - yifN b3777 0.16 -0.4 3,958,106 - yifN b3777 0.16 +0.0 3,958,111 + yifN b3777 0.17 +0.0 3,958,113 - yifN b3777 0.17 +0.4 3,958,113 - yifN b3777 0.17 +0.1 3,958,115 + yifN b3777 0.18 +0.7 3,958,126 + yifN b3777 0.20 +0.0 3,958,135 - yifN b3777 0.22 -0.2 3,958,135 - yifN b3777 0.22 -0.4 3,958,137 - yifN b3777 0.23 +0.0 3,958,141 + yifN b3777 0.24 +0.3 3,958,144 - yifN b3777 0.24 +0.2 3,958,154 - yifN b3777 0.27 +0.3 3,958,177 - yifN b3777 0.32 +0.1 3,958,185 - yifN b3777 0.33 -0.1 3,958,191 - yifN b3777 0.35 -0.5 3,958,191 - yifN b3777 0.35 -0.0 3,958,295 - yifN b3777 0.58 +0.0 3,958,307 - yifN b3777 0.61 -0.3 3,958,352 + yifN b3777 0.71 +0.7 3,958,391 - yifN b3777 0.79 -0.0 3,958,391 - yifN b3777 0.79 -0.4 3,958,393 - yifN b3777 0.80 +0.3 3,958,403 - yifN b3777 0.82 -0.0 3,958,403 - yifN b3777 0.82 -0.3 3,958,419 + yifN b3777 0.86 -0.1 3,958,435 - yifN b3777 0.89 +0.3 3,958,435 - yifN b3777 0.89 -0.2 3,958,459 + -0.2 3,958,483 + +0.6 3,958,529 + +0.2 3,958,659 + +0.3 3,958,659 + -0.3 3,958,705 + +0.5 3,958,904 - rep b3778 0.10 -0.3 3,958,904 - rep b3778 0.10 -1.3 3,958,931 + rep b3778 0.11 +0.1 3,958,942 + rep b3778 0.12 +0.1 3,958,950 - rep b3778 0.12 -0.6 3,958,961 + rep b3778 0.13 -0.5 3,958,966 + rep b3778 0.13 -1.2 3,958,970 - rep b3778 0.13 -0.5 3,958,974 - rep b3778 0.14 -1.8 3,959,079 - rep b3778 0.19 -0.3 3,959,079 - rep b3778 0.19 -0.1 3,959,093 - rep b3778 0.19 -0.7 3,959,113 - rep b3778 0.20 -0.5 3,959,195 + rep b3778 0.24 -0.3 3,959,207 - rep b3778 0.25 +0.1 3,959,207 - rep b3778 0.25 -0.3 3,959,214 - rep b3778 0.25 +0.3 3,959,215 - rep b3778 0.25 +1.1 3,959,221 - rep b3778 0.26 -0.4 3,959,222 - rep b3778 0.26 +0.1 3,959,222 - rep b3778 0.26 +0.1 3,959,229 + rep b3778 0.26 -0.2 3,959,281 - rep b3778 0.29 +0.1 3,959,351 + rep b3778 0.32 +0.6 3,959,351 + rep b3778 0.32 +0.1 3,959,352 - rep b3778 0.32 -0.7 3,959,352 - rep b3778 0.32 -1.3 3,959,361 - rep b3778 0.33 -2.5 3,959,361 - rep b3778 0.33 +0.7 3,959,371 - rep b3778 0.33 -0.9 3,959,375 + rep b3778 0.33 +0.2 3,959,376 - rep b3778 0.33 +0.7 3,959,409 + rep b3778 0.35 +0.1 3,959,414 - rep b3778 0.35 -1.7 3,959,419 + rep b3778 0.36 +0.1 3,959,454 + rep b3778 0.37 -0.9 3,959,454 + rep b3778 0.37 -0.2 3,959,460 - +1.1 3,959,460 - rep b3778 0.38 +0.1 3,959,509 + rep b3778 0.40 -0.9 3,959,514 + rep b3778 0.40 -0.3 3,959,524 + rep b3778 0.41 -1.8 3,959,527 - rep b3778 0.41 -0.2 3,959,541 + rep b3778 0.42 +0.5 3,959,588 - rep b3778 0.44 -0.3 3,959,597 - rep b3778 0.44 -0.5 3,959,612 + rep b3778 0.45 -2.5 3,959,625 - rep b3778 0.46 +1.1 3,959,625 - rep b3778 0.46 -0.5 3,959,729 + rep b3778 0.51 -1.7 3,959,786 + rep b3778 0.54 +0.1 3,959,865 + rep b3778 0.58 -0.3 3,959,886 - rep b3778 0.59 -1.5 3,959,886 - rep b3778 0.59 -0.3 3,959,891 - rep b3778 0.59 -1.3 3,959,940 + rep b3778 0.61 -0.7 3,960,053 + rep b3778 0.67 +0.7 3,960,053 + rep b3778 0.67 -1.3 3,960,056 - rep b3778 0.67 -1.5 3,960,056 - rep b3778 0.67 -1.3 3,960,076 - rep b3778 0.68 +2.1 3,960,154 + rep b3778 0.72 +0.5 3,960,154 + rep b3778 0.72 +1.1 3,960,243 + +2.1 3,960,276 - rep b3778 0.78 -0.9 3,960,290 + rep b3778 0.79 +1.1 3,960,358 + rep b3778 0.82 +0.1 3,960,366 + rep b3778 0.82 -0.7 3,960,422 - rep b3778 0.85 -0.9 3,960,465 - rep b3778 0.87 -3.3 3,960,519 - -1.3 3,960,530 - -1.2 3,960,530 - +1.5 3,960,532 + -1.9 3,960,532 + -1.9 3,960,532 + +0.1 3,960,575 - +0.1 3,960,740 + -0.4 3,960,799 - -0.0 3,960,837 - -0.1 3,960,867 - +0.3 3,960,889 + +0.1 3,960,895 + +0.7 3,960,895 + +1.1 3,960,896 + +0.8 3,960,975 - gpp b3779 0.14 -0.4 3,961,019 + gpp b3779 0.17 +0.3 3,961,038 + gpp b3779 0.18 +0.5 3,961,042 - gpp b3779 0.18 +0.5 3,961,052 + gpp b3779 0.19 -0.4 3,961,052 + gpp b3779 0.19 +0.6 3,961,052 + gpp b3779 0.19 +0.5 3,961,055 - gpp b3779 0.19 +0.9 3,961,055 - gpp b3779 0.19 +0.4 3,961,115 + gpp b3779 0.23 +0.2 3,961,131 - gpp b3779 0.24 -0.9 3,961,151 + gpp b3779 0.26 +1.2 3,961,277 + gpp b3779 0.34 +0.4 3,961,299 + gpp b3779 0.36 -0.3 3,961,314 - gpp b3779 0.37 -0.0 3,961,324 - gpp b3779 0.37 -0.5 3,961,324 - gpp b3779 0.37 -0.0 3,961,324 - gpp b3779 0.37 +0.3 3,961,326 - gpp b3779 0.38 -0.1 3,961,327 + gpp b3779 0.38 -0.0 3,961,360 + gpp b3779 0.40 -0.1 3,961,360 + gpp b3779 0.40 +0.4 3,961,365 + gpp b3779 0.40 +0.4 3,961,365 + gpp b3779 0.40 +0.1 3,961,382 + gpp b3779 0.41 -0.1 3,961,405 - gpp b3779 0.43 +0.7 3,961,407 - gpp b3779 0.43 +0.8 3,961,407 - gpp b3779 0.43 -0.2 3,961,407 - gpp b3779 0.43 +0.7 3,961,451 - gpp b3779 0.46 +0.8 3,961,451 - gpp b3779 0.46 +0.0 3,961,453 + gpp b3779 0.46 +0.4 3,961,473 + gpp b3779 0.47 +0.5 3,961,473 + gpp b3779 0.47 +1.3 3,961,481 - gpp b3779 0.48 +0.8 3,961,481 - gpp b3779 0.48 +1.4 3,961,501 + gpp b3779 0.49 +0.4 3,961,501 + gpp b3779 0.49 +0.7 3,961,523 - gpp b3779 0.51 +0.6 3,961,526 - gpp b3779 0.51 +0.4 3,961,532 + gpp b3779 0.51 +0.9 3,961,566 - gpp b3779 0.54 +0.6 3,961,571 - gpp b3779 0.54 +0.7 3,961,605 - gpp b3779 0.56 +0.8 3,961,605 - gpp b3779 0.56 +0.8 3,961,615 - gpp b3779 0.57 +0.5 3,961,615 - gpp b3779 0.57 +0.2 3,961,620 - gpp b3779 0.57 +0.5 3,961,621 - gpp b3779 0.57 +0.6 3,961,671 + gpp b3779 0.61 +0.5
Or see this region's nucleotide sequence