Strain Fitness in Escherichia coli BW25113 around b3965

Experiment: BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfabR and yijD overlap by 1 nucleotidesyijD and trmA are separated by 39 nucleotidestrmA and btuB are separated by 368 nucleotides b3963: fabR - DNA-binding transcriptional repressor (RefSeq), at 4,159,147 to 4,159,794 fabR b3964: yijD - conserved inner membrane protein (NCBI), at 4,159,794 to 4,160,153 yijD b3965: trmA - tRNA (uracil-5-)-methyltransferase (NCBI), at 4,160,193 to 4,161,293 trmA b3966: btuB - vitamin B12/cobalamin outer membrane transporter (NCBI), at 4,161,662 to 4,163,506 btuB Position (kb) 4160 4161 4162Strain fitness (log2 ratio) -2 -1 0 1 2at 4159.215 kb on - strand, within fabRat 4159.220 kb on - strand, within fabRat 4159.220 kb on - strand, within fabRat 4159.237 kb on - strand, within fabRat 4159.237 kb on - strand, within fabRat 4159.238 kb on + strand, within fabRat 4159.238 kb on + strand, within fabRat 4159.278 kb on - strand, within fabRat 4159.291 kb on - strand, within fabRat 4159.291 kb on - strand, within fabRat 4159.321 kb on + strand, within fabRat 4159.351 kb on + strand, within fabRat 4159.352 kb on + strand, within fabRat 4159.354 kb on - strand, within fabRat 4159.356 kb on + strand, within fabRat 4159.402 kb on + strand, within fabRat 4159.402 kb on + strand, within fabRat 4159.403 kb on + strand, within fabRat 4159.424 kb on + strand, within fabRat 4159.445 kb on + strand, within fabRat 4159.559 kb on + strand, within fabRat 4159.559 kb on + strand, within fabRat 4159.629 kb on + strand, within fabRat 4159.643 kb on - strand, within fabRat 4159.656 kb on - strand, within fabRat 4159.697 kb on + strand, within fabRat 4159.697 kb on + strand, within fabRat 4159.699 kb on - strand, within fabRat 4159.699 kb on - strand, within fabRat 4159.726 kb on + strand, within fabRat 4159.726 kb on + strand, within fabRat 4159.734 kb on - strandat 4159.789 kb on + strandat 4159.799 kb on - strandat 4159.811 kb on + strandat 4159.811 kb on + strandat 4159.822 kb on + strandat 4159.822 kb on + strandat 4159.824 kb on - strandat 4159.905 kb on - strand, within yijDat 4159.932 kb on + strand, within yijDat 4159.932 kb on + strand, within yijDat 4159.953 kb on + strand, within yijDat 4159.954 kb on + strand, within yijDat 4159.963 kb on + strand, within yijDat 4159.966 kb on + strand, within yijDat 4160.019 kb on + strand, within yijDat 4160.019 kb on + strand, within yijDat 4160.019 kb on + strand, within yijDat 4160.019 kb on + strand, within yijDat 4160.021 kb on + strand, within yijDat 4160.021 kb on + strand, within yijDat 4160.021 kb on + strand, within yijDat 4160.022 kb on + strand, within yijDat 4160.027 kb on - strand, within yijDat 4160.027 kb on - strand, within yijDat 4160.032 kb on - strand, within yijDat 4160.035 kb on - strand, within yijDat 4160.035 kb on - strand, within yijDat 4160.110 kb on - strand, within yijDat 4160.113 kb on + strand, within yijDat 4160.136 kb on - strandat 4160.177 kb on - strandat 4160.177 kb on - strandat 4160.190 kb on + strandat 4160.196 kb on - strandat 4160.205 kb on + strandat 4160.207 kb on + strandat 4160.207 kb on + strandat 4160.207 kb on + strandat 4160.222 kb on + strandat 4160.222 kb on + strandat 4160.229 kb on - strandat 4160.229 kb on - strandat 4160.236 kb on + strandat 4160.236 kb on + strandat 4160.241 kb on - strandat 4160.244 kb on - strandat 4160.244 kb on - strandat 4160.244 kb on - strandat 4160.244 kb on - strandat 4160.244 kb on - strandat 4160.279 kb on - strandat 4160.291 kb on - strandat 4160.313 kb on + strand, within trmAat 4160.313 kb on + strand, within trmAat 4160.352 kb on - strand, within trmAat 4160.366 kb on + strand, within trmAat 4160.371 kb on + strand, within trmAat 4160.384 kb on - strand, within trmAat 4160.391 kb on - strand, within trmAat 4160.470 kb on + strand, within trmAat 4160.530 kb on - strand, within trmAat 4160.544 kb on + strand, within trmAat 4160.547 kb on - strand, within trmAat 4160.547 kb on - strand, within trmAat 4160.549 kb on - strand, within trmAat 4160.555 kb on - strand, within trmAat 4160.568 kb on - strand, within trmAat 4160.648 kb on - strand, within trmAat 4160.673 kb on + strand, within trmAat 4160.673 kb on + strand, within trmAat 4160.686 kb on + strand, within trmAat 4160.687 kb on - strand, within trmAat 4160.687 kb on - strand, within trmAat 4160.734 kb on - strand, within trmAat 4160.751 kb on - strand, within trmAat 4160.767 kb on - strand, within trmAat 4160.767 kb on - strand, within trmAat 4160.781 kb on + strand, within trmAat 4160.781 kb on - strand, within trmAat 4160.799 kb on - strand, within trmAat 4160.877 kb on - strand, within trmAat 4160.882 kb on - strand, within trmAat 4160.914 kb on + strand, within trmAat 4160.921 kb on + strand, within trmAat 4160.921 kb on - strand, within trmAat 4160.922 kb on + strand, within trmAat 4160.930 kb on - strand, within trmAat 4160.930 kb on - strand, within trmAat 4160.952 kb on - strand, within trmAat 4160.954 kb on + strand, within trmAat 4160.954 kb on + strand, within trmAat 4160.962 kb on - strand, within trmAat 4160.969 kb on - strand, within trmAat 4160.969 kb on - strand, within trmAat 4160.983 kb on - strand, within trmAat 4160.993 kb on + strand, within trmAat 4161.011 kb on - strand, within trmAat 4161.011 kb on - strand, within trmAat 4161.013 kb on + strand, within trmAat 4161.013 kb on + strand, within trmAat 4161.021 kb on - strand, within trmAat 4161.021 kb on - strand, within trmAat 4161.055 kb on - strand, within trmAat 4161.103 kb on + strand, within trmAat 4161.139 kb on + strand, within trmAat 4161.149 kb on - strand, within trmAat 4161.161 kb on + strand, within trmAat 4161.164 kb on - strand, within trmAat 4161.215 kb on + strandat 4161.223 kb on - strandat 4161.259 kb on + strandat 4161.264 kb on - strandat 4161.327 kb on + strandat 4161.337 kb on + strandat 4161.390 kb on - strandat 4161.436 kb on - strandat 4161.446 kb on + strandat 4161.448 kb on - strandat 4161.448 kb on - strandat 4161.537 kb on - strandat 4161.537 kb on - strandat 4161.538 kb on - strandat 4161.548 kb on - strandat 4161.548 kb on - strandat 4161.564 kb on + strandat 4161.564 kb on + strandat 4161.572 kb on - strandat 4161.609 kb on + strandat 4161.618 kb on - strandat 4161.618 kb on - strandat 4161.622 kb on - strandat 4161.622 kb on - strandat 4161.722 kb on - strandat 4161.741 kb on + strandat 4161.741 kb on + strandat 4161.783 kb on - strandat 4161.783 kb on - strandat 4161.791 kb on + strandat 4161.794 kb on - strandat 4161.826 kb on - strandat 4161.826 kb on - strandat 4161.836 kb on - strandat 4161.907 kb on + strand, within btuBat 4161.949 kb on + strand, within btuBat 4161.949 kb on + strand, within btuBat 4161.994 kb on - strand, within btuBat 4161.994 kb on - strand, within btuBat 4161.996 kb on - strand, within btuBat 4162.005 kb on + strand, within btuBat 4162.013 kb on - strand, within btuBat 4162.017 kb on + strand, within btuBat 4162.017 kb on + strand, within btuBat 4162.023 kb on - strand, within btuBat 4162.028 kb on - strand, within btuBat 4162.050 kb on + strand, within btuBat 4162.053 kb on - strand, within btuBat 4162.070 kb on + strand, within btuBat 4162.078 kb on - strand, within btuBat 4162.107 kb on - strand, within btuBat 4162.145 kb on + strand, within btuBat 4162.148 kb on + strand, within btuBat 4162.174 kb on + strand, within btuBat 4162.182 kb on + strand, within btuBat 4162.182 kb on + strand, within btuBat 4162.190 kb on + strand, within btuBat 4162.190 kb on - strand, within btuBat 4162.190 kb on - strand, within btuBat 4162.202 kb on + strand, within btuBat 4162.237 kb on + strand, within btuBat 4162.265 kb on + strand, within btuBat 4162.270 kb on + strand, within btuBat 4162.278 kb on - strand, within btuB

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
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4,159,215 - fabR b3963 0.10 +0.3
4,159,220 - fabR b3963 0.11 -0.4
4,159,220 - fabR b3963 0.11 +0.0
4,159,237 - fabR b3963 0.14 -0.7
4,159,237 - fabR b3963 0.14 -0.3
4,159,238 + fabR b3963 0.14 -0.5
4,159,238 + fabR b3963 0.14 -1.3
4,159,278 - fabR b3963 0.20 -0.4
4,159,291 - fabR b3963 0.22 -0.1
4,159,291 - fabR b3963 0.22 +0.0
4,159,321 + fabR b3963 0.27 -0.4
4,159,351 + fabR b3963 0.31 -0.2
4,159,352 + fabR b3963 0.32 -0.3
4,159,354 - fabR b3963 0.32 +0.1
4,159,356 + fabR b3963 0.32 -0.1
4,159,402 + fabR b3963 0.39 -0.3
4,159,402 + fabR b3963 0.39 -0.5
4,159,403 + fabR b3963 0.40 -0.0
4,159,424 + fabR b3963 0.43 +0.0
4,159,445 + fabR b3963 0.46 +0.2
4,159,559 + fabR b3963 0.64 +0.4
4,159,559 + fabR b3963 0.64 -0.7
4,159,629 + fabR b3963 0.74 +0.3
4,159,643 - fabR b3963 0.77 +0.1
4,159,656 - fabR b3963 0.79 +0.1
4,159,697 + fabR b3963 0.85 -0.7
4,159,697 + fabR b3963 0.85 +0.1
4,159,699 - fabR b3963 0.85 +0.1
4,159,699 - fabR b3963 0.85 -0.1
4,159,726 + fabR b3963 0.89 +0.5
4,159,726 + fabR b3963 0.89 +0.0
4,159,734 - +1.1
4,159,789 + +0.2
4,159,799 - +0.2
4,159,811 + +1.0
4,159,811 + -0.0
4,159,822 + +0.0
4,159,822 + +0.1
4,159,824 - +0.6
4,159,905 - yijD b3964 0.31 +0.0
4,159,932 + yijD b3964 0.38 +0.3
4,159,932 + yijD b3964 0.38 +0.2
4,159,953 + yijD b3964 0.44 -0.2
4,159,954 + yijD b3964 0.44 -0.0
4,159,963 + yijD b3964 0.47 +0.4
4,159,966 + yijD b3964 0.48 +0.0
4,160,019 + yijD b3964 0.62 -0.3
4,160,019 + yijD b3964 0.62 +0.5
4,160,019 + yijD b3964 0.62 +0.2
4,160,019 + yijD b3964 0.62 -0.1
4,160,021 + yijD b3964 0.63 -0.1
4,160,021 + yijD b3964 0.63 -0.0
4,160,021 + yijD b3964 0.63 +0.6
4,160,022 + yijD b3964 0.63 +0.1
4,160,027 - yijD b3964 0.65 -0.5
4,160,027 - yijD b3964 0.65 -0.1
4,160,032 - yijD b3964 0.66 +2.7
4,160,035 - yijD b3964 0.67 -0.0
4,160,035 - yijD b3964 0.67 -0.2
4,160,110 - yijD b3964 0.88 -0.7
4,160,113 + yijD b3964 0.89 +0.1
4,160,136 - +0.7
4,160,177 - -0.5
4,160,177 - -0.1
4,160,190 + +0.0
4,160,196 - +0.3
4,160,205 + +0.1
4,160,207 + -0.4
4,160,207 + -0.2
4,160,207 + -1.5
4,160,222 + -0.6
4,160,222 + -0.2
4,160,229 - -1.0
4,160,229 - -0.6
4,160,236 + -0.5
4,160,236 + -0.2
4,160,241 - +0.1
4,160,244 - -1.5
4,160,244 - -0.1
4,160,244 - -0.5
4,160,244 - -1.0
4,160,244 - +0.3
4,160,279 - -0.7
4,160,291 - +0.1
4,160,313 + trmA b3965 0.11 -0.5
4,160,313 + trmA b3965 0.11 -1.3
4,160,352 - trmA b3965 0.14 -1.1
4,160,366 + trmA b3965 0.16 -0.6
4,160,371 + trmA b3965 0.16 -0.8
4,160,384 - trmA b3965 0.17 -0.9
4,160,391 - trmA b3965 0.18 -0.9
4,160,470 + trmA b3965 0.25 -0.4
4,160,530 - trmA b3965 0.31 -0.2
4,160,544 + trmA b3965 0.32 +0.6
4,160,547 - trmA b3965 0.32 -0.7
4,160,547 - trmA b3965 0.32 -1.1
4,160,549 - trmA b3965 0.32 -1.0
4,160,555 - trmA b3965 0.33 -1.1
4,160,568 - trmA b3965 0.34 +0.2
4,160,648 - trmA b3965 0.41 -1.0
4,160,673 + trmA b3965 0.44 -0.3
4,160,673 + trmA b3965 0.44 -1.3
4,160,686 + trmA b3965 0.45 -0.6
4,160,687 - trmA b3965 0.45 -1.0
4,160,687 - trmA b3965 0.45 -1.4
4,160,734 - trmA b3965 0.49 +0.0
4,160,751 - trmA b3965 0.51 -0.7
4,160,767 - trmA b3965 0.52 +0.1
4,160,767 - trmA b3965 0.52 +0.1
4,160,781 + trmA b3965 0.53 -0.8
4,160,781 - trmA b3965 0.53 -0.8
4,160,799 - trmA b3965 0.55 -1.1
4,160,877 - trmA b3965 0.62 -0.9
4,160,882 - trmA b3965 0.63 -0.4
4,160,914 + trmA b3965 0.65 -0.6
4,160,921 + trmA b3965 0.66 -0.5
4,160,921 - trmA b3965 0.66 -1.0
4,160,922 + trmA b3965 0.66 -0.6
4,160,930 - trmA b3965 0.67 +1.4
4,160,930 - trmA b3965 0.67 -0.7
4,160,952 - trmA b3965 0.69 -0.7
4,160,954 + trmA b3965 0.69 -1.0
4,160,954 + trmA b3965 0.69 -1.2
4,160,962 - trmA b3965 0.70 -1.0
4,160,969 - trmA b3965 0.70 -0.2
4,160,969 - trmA b3965 0.70 -0.0
4,160,983 - trmA b3965 0.72 -0.9
4,160,993 + trmA b3965 0.73 +0.2
4,161,011 - trmA b3965 0.74 -1.0
4,161,011 - trmA b3965 0.74 -0.8
4,161,013 + trmA b3965 0.74 -0.5
4,161,013 + trmA b3965 0.74 -0.0
4,161,021 - trmA b3965 0.75 -0.7
4,161,021 - trmA b3965 0.75 -0.9
4,161,055 - trmA b3965 0.78 -1.8
4,161,103 + trmA b3965 0.83 -0.2
4,161,139 + trmA b3965 0.86 -0.5
4,161,149 - trmA b3965 0.87 -1.2
4,161,161 + trmA b3965 0.88 -0.5
4,161,164 - trmA b3965 0.88 -0.5
4,161,215 + -0.4
4,161,223 - -0.5
4,161,259 + -0.4
4,161,264 - +0.1
4,161,327 + -0.7
4,161,337 + -0.1
4,161,390 - -0.1
4,161,436 - -0.2
4,161,446 + +0.1
4,161,448 - -0.4
4,161,448 - +1.1
4,161,537 - +0.4
4,161,537 - +0.2
4,161,538 - +0.1
4,161,548 - +1.2
4,161,548 - +0.7
4,161,564 + +0.4
4,161,564 + -0.1
4,161,572 - +0.2
4,161,609 + -0.1
4,161,618 - +0.2
4,161,618 - +0.2
4,161,622 - +0.1
4,161,622 - +0.4
4,161,722 - -0.1
4,161,741 + +1.6
4,161,741 + +0.1
4,161,783 - +0.1
4,161,783 - +0.3
4,161,791 + +0.2
4,161,794 - -0.5
4,161,826 - -0.1
4,161,826 - -0.0
4,161,836 - +0.1
4,161,907 + btuB b3966 0.13 -0.2
4,161,949 + btuB b3966 0.16 -0.2
4,161,949 + btuB b3966 0.16 -0.5
4,161,994 - btuB b3966 0.18 -0.3
4,161,994 - btuB b3966 0.18 -0.4
4,161,996 - btuB b3966 0.18 +0.7
4,162,005 + btuB b3966 0.19 +0.0
4,162,013 - btuB b3966 0.19 -0.3
4,162,017 + btuB b3966 0.19 +0.1
4,162,017 + btuB b3966 0.19 -0.0
4,162,023 - btuB b3966 0.20 -0.2
4,162,028 - btuB b3966 0.20 -0.3
4,162,050 + btuB b3966 0.21 +0.7
4,162,053 - btuB b3966 0.21 -0.2
4,162,070 + btuB b3966 0.22 -0.2
4,162,078 - btuB b3966 0.23 -0.3
4,162,107 - btuB b3966 0.24 -0.7
4,162,145 + btuB b3966 0.26 -0.2
4,162,148 + btuB b3966 0.26 -0.0
4,162,174 + btuB b3966 0.28 -0.4
4,162,182 + btuB b3966 0.28 -1.1
4,162,182 + btuB b3966 0.28 +0.6
4,162,190 + btuB b3966 0.29 +0.4
4,162,190 - btuB b3966 0.29 +0.1
4,162,190 - btuB b3966 0.29 +0.2
4,162,202 + btuB b3966 0.29 -0.0
4,162,237 + btuB b3966 0.31 +0.7
4,162,265 + btuB b3966 0.33 -0.2
4,162,270 + btuB b3966 0.33 +0.1
4,162,278 - btuB b3966 0.33 -0.0

Or see this region's nucleotide sequence