Experiment: BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pitA and yhiO are separated by 243 nucleotides yhiO and uspA are separated by 390 nucleotides
b3493: pitA - phosphate transporter, low-affinity (NCBI), at 3,635,665 to 3,637,164
pitA
b3494: yhiO - universal stress protein UspB (NCBI), at 3,637,408 to 3,637,743
yhiO
b3495: uspA - universal stress global response regulator (NCBI), at 3,638,134 to 3,638,568
uspA
Position (kb)
3637
3638 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3636.470 kb on - strand, within pitA at 3636.477 kb on + strand, within pitA at 3636.479 kb on - strand, within pitA at 3636.494 kb on + strand, within pitA at 3636.494 kb on + strand, within pitA at 3636.494 kb on + strand, within pitA at 3636.495 kb on - strand, within pitA at 3636.495 kb on - strand, within pitA at 3636.502 kb on - strand, within pitA at 3636.502 kb on - strand, within pitA at 3636.504 kb on + strand, within pitA at 3636.504 kb on + strand, within pitA at 3636.521 kb on - strand, within pitA at 3636.578 kb on + strand, within pitA at 3636.578 kb on + strand, within pitA at 3636.604 kb on + strand, within pitA at 3636.657 kb on + strand, within pitA at 3636.665 kb on - strand, within pitA at 3636.684 kb on - strand, within pitA at 3636.697 kb on - strand, within pitA at 3636.697 kb on - strand, within pitA at 3636.721 kb on - strand, within pitA at 3636.736 kb on + strand, within pitA at 3636.748 kb on - strand, within pitA at 3636.773 kb on - strand, within pitA at 3636.773 kb on - strand, within pitA at 3636.797 kb on - strand, within pitA at 3636.805 kb on - strand, within pitA at 3636.805 kb on - strand, within pitA at 3636.812 kb on - strand, within pitA at 3636.821 kb on - strand, within pitA at 3636.822 kb on - strand, within pitA at 3636.822 kb on - strand, within pitA at 3636.854 kb on + strand, within pitA at 3636.856 kb on - strand, within pitA at 3636.856 kb on - strand, within pitA at 3636.865 kb on + strand, within pitA at 3636.865 kb on + strand, within pitA at 3636.865 kb on + strand, within pitA at 3636.887 kb on - strand, within pitA at 3636.913 kb on + strand, within pitA at 3636.919 kb on + strand, within pitA at 3636.920 kb on + strand, within pitA at 3636.921 kb on - strand, within pitA at 3636.948 kb on - strand, within pitA at 3636.960 kb on + strand, within pitA at 3636.986 kb on + strand, within pitA at 3636.993 kb on + strand, within pitA at 3637.004 kb on + strand at 3637.004 kb on + strand, within pitA at 3637.004 kb on + strand, within pitA at 3637.009 kb on - strand, within pitA at 3637.011 kb on - strand, within pitA at 3637.022 kb on - strand at 3637.039 kb on + strand at 3637.039 kb on + strand at 3637.041 kb on + strand at 3637.046 kb on - strand at 3637.054 kb on + strand at 3637.054 kb on + strand at 3637.062 kb on + strand at 3637.062 kb on + strand at 3637.062 kb on - strand at 3637.070 kb on - strand at 3637.070 kb on - strand at 3637.076 kb on + strand at 3637.090 kb on + strand at 3637.092 kb on - strand at 3637.097 kb on - strand at 3637.106 kb on - strand at 3637.109 kb on + strand at 3637.135 kb on + strand at 3637.172 kb on + strand at 3637.205 kb on + strand at 3637.205 kb on + strand at 3637.215 kb on - strand at 3637.215 kb on - strand at 3637.234 kb on - strand at 3637.312 kb on - strand at 3637.312 kb on - strand at 3637.314 kb on + strand at 3637.317 kb on - strand at 3637.367 kb on - strand at 3637.372 kb on - strand at 3637.401 kb on + strand at 3637.443 kb on + strand, within yhiO at 3637.443 kb on + strand, within yhiO at 3637.443 kb on + strand, within yhiO at 3637.443 kb on + strand, within yhiO at 3637.446 kb on - strand, within yhiO at 3637.454 kb on + strand, within yhiO at 3637.454 kb on + strand, within yhiO at 3637.459 kb on - strand, within yhiO at 3637.459 kb on - strand, within yhiO at 3637.463 kb on - strand, within yhiO at 3637.468 kb on - strand, within yhiO at 3637.487 kb on - strand, within yhiO at 3637.496 kb on - strand, within yhiO at 3637.529 kb on + strand, within yhiO at 3637.539 kb on + strand, within yhiO at 3637.558 kb on - strand, within yhiO at 3637.577 kb on - strand, within yhiO at 3637.579 kb on - strand, within yhiO at 3637.614 kb on + strand, within yhiO at 3637.650 kb on - strand, within yhiO at 3637.655 kb on - strand, within yhiO at 3637.670 kb on + strand, within yhiO at 3637.715 kb on + strand at 3637.733 kb on + strand at 3637.735 kb on + strand at 3637.741 kb on - strand at 3637.741 kb on - strand at 3637.741 kb on - strand at 3637.746 kb on - strand at 3637.780 kb on - strand at 3637.783 kb on + strand at 3637.824 kb on + strand at 3637.832 kb on - strand at 3637.854 kb on + strand at 3637.854 kb on + strand at 3637.865 kb on + strand at 3637.865 kb on + strand at 3637.873 kb on - strand at 3637.875 kb on - strand at 3637.875 kb on - strand at 3637.875 kb on - strand at 3637.877 kb on + strand at 3637.877 kb on + strand at 3637.878 kb on - strand at 3637.909 kb on + strand at 3637.909 kb on + strand at 3637.926 kb on - strand at 3637.969 kb on - strand at 3638.006 kb on - strand at 3638.014 kb on + strand at 3638.033 kb on + strand at 3638.053 kb on - strand at 3638.079 kb on - strand at 3638.083 kb on + strand at 3638.090 kb on - strand at 3638.100 kb on - strand at 3638.203 kb on - strand, within uspA at 3638.203 kb on - strand, within uspA at 3638.205 kb on + strand, within uspA at 3638.207 kb on + strand, within uspA at 3638.207 kb on + strand, within uspA at 3638.211 kb on - strand, within uspA at 3638.213 kb on - strand, within uspA at 3638.215 kb on + strand, within uspA at 3638.215 kb on + strand, within uspA at 3638.225 kb on - strand, within uspA at 3638.266 kb on + strand, within uspA at 3638.266 kb on + strand, within uspA at 3638.266 kb on + strand, within uspA at 3638.266 kb on + strand, within uspA at 3638.268 kb on + strand, within uspA at 3638.274 kb on - strand, within uspA at 3638.276 kb on - strand, within uspA at 3638.318 kb on + strand, within uspA at 3638.333 kb on + strand, within uspA at 3638.333 kb on + strand, within uspA at 3638.338 kb on + strand, within uspA at 3638.345 kb on - strand, within uspA at 3638.345 kb on - strand, within uspA at 3638.383 kb on + strand, within uspA at 3638.386 kb on + strand, within uspA at 3638.386 kb on + strand, within uspA at 3638.395 kb on + strand, within uspA at 3638.395 kb on + strand, within uspA at 3638.408 kb on + strand, within uspA at 3638.408 kb on + strand, within uspA at 3638.412 kb on + strand, within uspA at 3638.412 kb on + strand, within uspA at 3638.455 kb on - strand, within uspA at 3638.495 kb on + strand, within uspA at 3638.511 kb on + strand, within uspA at 3638.511 kb on + strand, within uspA at 3638.519 kb on - strand, within uspA at 3638.530 kb on + strand at 3638.536 kb on - strand at 3638.536 kb on - strand at 3638.542 kb on + strand at 3638.550 kb on - strand at 3638.561 kb on - strand at 3638.582 kb on + strand at 3638.582 kb on + strand at 3638.630 kb on + strand at 3638.638 kb on + strand at 3638.639 kb on - strand at 3638.639 kb on - strand at 3638.649 kb on - strand at 3638.649 kb on - strand at 3638.675 kb on + strand at 3638.683 kb on - strand at 3638.683 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr remove 3,636,470 - pitA b3493 0.54 -1.7 3,636,477 + pitA b3493 0.54 -3.1 3,636,479 - pitA b3493 0.54 -2.0 3,636,494 + pitA b3493 0.55 +0.0 3,636,494 + pitA b3493 0.55 -2.3 3,636,494 + pitA b3493 0.55 -2.3 3,636,495 - pitA b3493 0.55 -1.6 3,636,495 - pitA b3493 0.55 -2.7 3,636,502 - pitA b3493 0.56 -1.8 3,636,502 - pitA b3493 0.56 +0.0 3,636,504 + pitA b3493 0.56 -0.6 3,636,504 + pitA b3493 0.56 -1.3 3,636,521 - pitA b3493 0.57 -1.0 3,636,578 + pitA b3493 0.61 -1.3 3,636,578 + pitA b3493 0.61 +0.4 3,636,604 + pitA b3493 0.63 -2.4 3,636,657 + pitA b3493 0.66 +1.0 3,636,665 - pitA b3493 0.67 -0.6 3,636,684 - pitA b3493 0.68 -1.3 3,636,697 - pitA b3493 0.69 -0.6 3,636,697 - pitA b3493 0.69 -0.8 3,636,721 - pitA b3493 0.70 +1.0 3,636,736 + pitA b3493 0.71 -2.0 3,636,748 - pitA b3493 0.72 -1.0 3,636,773 - pitA b3493 0.74 -2.1 3,636,773 - pitA b3493 0.74 -1.3 3,636,797 - pitA b3493 0.75 -1.8 3,636,805 - pitA b3493 0.76 -0.6 3,636,805 - pitA b3493 0.76 -0.8 3,636,812 - pitA b3493 0.76 -0.8 3,636,821 - pitA b3493 0.77 -0.8 3,636,822 - pitA b3493 0.77 +1.0 3,636,822 - pitA b3493 0.77 -1.6 3,636,854 + pitA b3493 0.79 -0.7 3,636,856 - pitA b3493 0.79 +1.0 3,636,856 - pitA b3493 0.79 -0.6 3,636,865 + pitA b3493 0.80 +0.0 3,636,865 + pitA b3493 0.80 -1.0 3,636,865 + pitA b3493 0.80 +0.0 3,636,887 - pitA b3493 0.81 -1.2 3,636,913 + pitA b3493 0.83 +0.0 3,636,919 + pitA b3493 0.84 -2.0 3,636,920 + pitA b3493 0.84 -1.0 3,636,921 - pitA b3493 0.84 -1.3 3,636,948 - pitA b3493 0.86 -1.0 3,636,960 + pitA b3493 0.86 -1.4 3,636,986 + pitA b3493 0.88 -1.0 3,636,993 + pitA b3493 0.89 +1.8 3,637,004 + +0.0 3,637,004 + pitA b3493 0.89 +0.0 3,637,004 + pitA b3493 0.89 -1.6 3,637,009 - pitA b3493 0.90 -0.6 3,637,011 - pitA b3493 0.90 -2.0 3,637,022 - -1.0 3,637,039 + +0.4 3,637,039 + -1.3 3,637,041 + -0.6 3,637,046 - -1.0 3,637,054 + -1.8 3,637,054 + -1.6 3,637,062 + -1.4 3,637,062 + -2.7 3,637,062 - +0.0 3,637,070 - +1.0 3,637,070 - -0.6 3,637,076 + -0.6 3,637,090 + -3.4 3,637,092 - -0.3 3,637,097 - +0.0 3,637,106 - -1.1 3,637,109 + +1.0 3,637,135 + -0.6 3,637,172 + -0.1 3,637,205 + -0.5 3,637,205 + -0.4 3,637,215 - -0.0 3,637,215 - -0.7 3,637,234 - +0.1 3,637,312 - +0.9 3,637,312 - +0.6 3,637,314 + -0.1 3,637,317 - +0.6 3,637,367 - -0.1 3,637,372 - -0.2 3,637,401 + -0.3 3,637,443 + yhiO b3494 0.10 -1.1 3,637,443 + yhiO b3494 0.10 -0.1 3,637,443 + yhiO b3494 0.10 -0.7 3,637,443 + yhiO b3494 0.10 -0.4 3,637,446 - yhiO b3494 0.11 -0.9 3,637,454 + yhiO b3494 0.14 -0.0 3,637,454 + yhiO b3494 0.14 -0.8 3,637,459 - yhiO b3494 0.15 -1.2 3,637,459 - yhiO b3494 0.15 -1.1 3,637,463 - yhiO b3494 0.16 +0.0 3,637,468 - yhiO b3494 0.18 +0.7 3,637,487 - yhiO b3494 0.24 -0.6 3,637,496 - yhiO b3494 0.26 +0.3 3,637,529 + yhiO b3494 0.36 -0.4 3,637,539 + yhiO b3494 0.39 -0.0 3,637,558 - yhiO b3494 0.45 -0.3 3,637,577 - yhiO b3494 0.50 -0.6 3,637,579 - yhiO b3494 0.51 -1.6 3,637,614 + yhiO b3494 0.61 -0.5 3,637,650 - yhiO b3494 0.72 -2.2 3,637,655 - yhiO b3494 0.74 -1.1 3,637,670 + yhiO b3494 0.78 -1.2 3,637,715 + +1.0 3,637,733 + -0.1 3,637,735 + -0.3 3,637,741 - -0.7 3,637,741 - +0.0 3,637,741 - -0.9 3,637,746 - -0.5 3,637,780 - -0.5 3,637,783 + -0.3 3,637,824 + -0.1 3,637,832 - -0.7 3,637,854 + -0.7 3,637,854 + -0.6 3,637,865 + -0.8 3,637,865 + -0.9 3,637,873 - -0.6 3,637,875 - -0.2 3,637,875 - -0.8 3,637,875 - +0.1 3,637,877 + -0.4 3,637,877 + -0.1 3,637,878 - -0.6 3,637,909 + +1.0 3,637,909 + -0.2 3,637,926 - +0.2 3,637,969 - -0.3 3,638,006 - +0.5 3,638,014 + +0.9 3,638,033 + +0.7 3,638,053 - +0.6 3,638,079 - -0.1 3,638,083 + -0.0 3,638,090 - +0.2 3,638,100 - -0.2 3,638,203 - uspA b3495 0.16 +0.6 3,638,203 - uspA b3495 0.16 -0.1 3,638,205 + uspA b3495 0.16 +0.9 3,638,207 + uspA b3495 0.17 +0.3 3,638,207 + uspA b3495 0.17 +0.4 3,638,211 - uspA b3495 0.18 +0.5 3,638,213 - uspA b3495 0.18 +0.7 3,638,215 + uspA b3495 0.19 +0.0 3,638,215 + uspA b3495 0.19 +0.0 3,638,225 - uspA b3495 0.21 +0.4 3,638,266 + uspA b3495 0.30 +0.6 3,638,266 + uspA b3495 0.30 +0.4 3,638,266 + uspA b3495 0.30 +0.5 3,638,266 + uspA b3495 0.30 +0.6 3,638,268 + uspA b3495 0.31 +0.5 3,638,274 - uspA b3495 0.32 +0.5 3,638,276 - uspA b3495 0.33 +0.4 3,638,318 + uspA b3495 0.42 +0.7 3,638,333 + uspA b3495 0.46 +0.5 3,638,333 + uspA b3495 0.46 +0.5 3,638,338 + uspA b3495 0.47 +0.5 3,638,345 - uspA b3495 0.49 +0.8 3,638,345 - uspA b3495 0.49 +0.8 3,638,383 + uspA b3495 0.57 +0.6 3,638,386 + uspA b3495 0.58 +0.5 3,638,386 + uspA b3495 0.58 +0.6 3,638,395 + uspA b3495 0.60 +0.3 3,638,395 + uspA b3495 0.60 +0.4 3,638,408 + uspA b3495 0.63 +0.5 3,638,408 + uspA b3495 0.63 +0.5 3,638,412 + uspA b3495 0.64 +0.8 3,638,412 + uspA b3495 0.64 +0.4 3,638,455 - uspA b3495 0.74 +0.3 3,638,495 + uspA b3495 0.83 +1.0 3,638,511 + uspA b3495 0.87 +1.0 3,638,511 + uspA b3495 0.87 +0.6 3,638,519 - uspA b3495 0.89 +0.6 3,638,530 + +0.7 3,638,536 - +0.3 3,638,536 - +0.4 3,638,542 + +0.2 3,638,550 - +0.7 3,638,561 - -0.5 3,638,582 + -0.4 3,638,582 + -0.3 3,638,630 + -0.6 3,638,638 + -0.1 3,638,639 - -0.2 3,638,639 - -0.0 3,638,649 - +0.2 3,638,649 - -0.2 3,638,675 + +0.7 3,638,683 - -0.5 3,638,683 - -0.8
Or see this region's nucleotide sequence