Experiment: BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fmt and rsmB are separated by 45 nucleotides rsmB and trkA are separated by 21 nucleotides
b3288: fmt - methionyl-tRNA formyltransferase (NCBI), at 3,432,236 to 3,433,183
fmt
b3289: rsmB - 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI), at 3,433,229 to 3,434,518
rsmB
b3290: trkA - potassium transporter peripheral membrane component (NCBI), at 3,434,540 to 3,435,916
trkA
Position (kb)
3433
3434
3435 Strain fitness (log2 ratio)
-2
-1
0
1 at 3432.238 kb on + strand at 3433.170 kb on + strand at 3433.177 kb on + strand at 3433.178 kb on + strand at 3433.186 kb on - strand at 3433.186 kb on - strand at 3433.193 kb on + strand at 3433.193 kb on + strand at 3433.218 kb on + strand at 3433.260 kb on - strand at 3433.293 kb on + strand at 3433.374 kb on + strand, within rsmB at 3433.374 kb on + strand, within rsmB at 3433.374 kb on + strand, within rsmB at 3433.379 kb on - strand, within rsmB at 3433.384 kb on - strand, within rsmB at 3433.392 kb on - strand, within rsmB at 3433.393 kb on + strand, within rsmB at 3433.397 kb on + strand, within rsmB at 3433.397 kb on + strand, within rsmB at 3433.397 kb on + strand, within rsmB at 3433.397 kb on + strand, within rsmB at 3433.397 kb on + strand, within rsmB at 3433.397 kb on + strand, within rsmB at 3433.421 kb on - strand, within rsmB at 3433.421 kb on - strand, within rsmB at 3433.440 kb on - strand, within rsmB at 3433.447 kb on + strand, within rsmB at 3433.464 kb on + strand, within rsmB at 3433.466 kb on - strand, within rsmB at 3433.472 kb on - strand, within rsmB at 3433.497 kb on + strand, within rsmB at 3433.504 kb on + strand, within rsmB at 3433.511 kb on - strand, within rsmB at 3433.518 kb on - strand, within rsmB at 3433.523 kb on - strand, within rsmB at 3433.523 kb on - strand, within rsmB at 3433.525 kb on + strand, within rsmB at 3433.525 kb on + strand, within rsmB at 3433.567 kb on + strand, within rsmB at 3433.589 kb on + strand, within rsmB at 3433.629 kb on - strand, within rsmB at 3433.665 kb on + strand, within rsmB at 3433.665 kb on + strand, within rsmB at 3433.665 kb on + strand, within rsmB at 3433.671 kb on - strand, within rsmB at 3433.671 kb on - strand, within rsmB at 3433.673 kb on - strand, within rsmB at 3433.698 kb on + strand, within rsmB at 3433.706 kb on - strand, within rsmB at 3433.706 kb on - strand, within rsmB at 3433.706 kb on - strand, within rsmB at 3433.758 kb on + strand, within rsmB at 3433.837 kb on + strand, within rsmB at 3433.843 kb on + strand, within rsmB at 3433.843 kb on + strand, within rsmB at 3433.845 kb on - strand, within rsmB at 3433.845 kb on - strand, within rsmB at 3433.851 kb on + strand, within rsmB at 3433.859 kb on - strand, within rsmB at 3433.864 kb on - strand, within rsmB at 3433.864 kb on - strand, within rsmB at 3433.864 kb on - strand, within rsmB at 3433.877 kb on + strand, within rsmB at 3433.936 kb on + strand, within rsmB at 3433.939 kb on - strand, within rsmB at 3433.944 kb on - strand, within rsmB at 3433.953 kb on - strand, within rsmB at 3434.019 kb on + strand, within rsmB at 3434.027 kb on - strand, within rsmB at 3434.036 kb on + strand, within rsmB at 3434.071 kb on - strand, within rsmB at 3434.071 kb on - strand, within rsmB at 3434.112 kb on - strand, within rsmB at 3434.134 kb on - strand at 3434.141 kb on - strand, within rsmB at 3434.141 kb on - strand, within rsmB at 3434.147 kb on - strand, within rsmB at 3434.147 kb on - strand, within rsmB at 3434.150 kb on + strand, within rsmB at 3434.158 kb on - strand, within rsmB at 3434.333 kb on + strand, within rsmB at 3434.339 kb on - strand, within rsmB at 3434.350 kb on - strand, within rsmB at 3434.350 kb on - strand, within rsmB at 3434.352 kb on + strand, within rsmB at 3434.357 kb on + strand, within rsmB at 3434.366 kb on + strand, within rsmB at 3434.370 kb on + strand, within rsmB at 3434.402 kb on + strand at 3434.430 kb on + strand at 3434.430 kb on + strand at 3434.432 kb on + strand at 3434.432 kb on + strand at 3434.433 kb on + strand at 3434.433 kb on + strand at 3434.471 kb on + strand at 3434.477 kb on - strand at 3434.477 kb on - strand at 3434.504 kb on - strand at 3434.504 kb on - strand at 3434.673 kb on + strand at 3434.812 kb on - strand, within trkA at 3434.850 kb on + strand, within trkA at 3434.913 kb on - strand, within trkA at 3434.918 kb on - strand, within trkA at 3434.955 kb on - strand, within trkA at 3434.955 kb on - strand, within trkA at 3435.049 kb on + strand, within trkA at 3435.185 kb on + strand, within trkA at 3435.275 kb on + strand, within trkA at 3435.314 kb on - strand, within trkA at 3435.416 kb on + strand, within trkA at 3435.474 kb on - strand, within trkA at 3435.476 kb on - strand, within trkA
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr remove 3,432,238 + +0.6 3,433,170 + -0.0 3,433,177 + -0.5 3,433,178 + +0.2 3,433,186 - -1.0 3,433,186 - +0.6 3,433,193 + -0.3 3,433,193 + +1.1 3,433,218 + -0.2 3,433,260 - -0.3 3,433,293 + -1.8 3,433,374 + rsmB b3289 0.11 -1.2 3,433,374 + rsmB b3289 0.11 -0.8 3,433,374 + rsmB b3289 0.11 -0.5 3,433,379 - rsmB b3289 0.12 +0.8 3,433,384 - rsmB b3289 0.12 -0.4 3,433,392 - rsmB b3289 0.13 +0.3 3,433,393 + rsmB b3289 0.13 -0.6 3,433,397 + rsmB b3289 0.13 -0.6 3,433,397 + rsmB b3289 0.13 +0.1 3,433,397 + rsmB b3289 0.13 -0.3 3,433,397 + rsmB b3289 0.13 -1.3 3,433,397 + rsmB b3289 0.13 -0.9 3,433,397 + rsmB b3289 0.13 -0.5 3,433,421 - rsmB b3289 0.15 -0.6 3,433,421 - rsmB b3289 0.15 -1.9 3,433,440 - rsmB b3289 0.16 -0.5 3,433,447 + rsmB b3289 0.17 -0.3 3,433,464 + rsmB b3289 0.18 -1.1 3,433,466 - rsmB b3289 0.18 -1.0 3,433,472 - rsmB b3289 0.19 -0.5 3,433,497 + rsmB b3289 0.21 +1.0 3,433,504 + rsmB b3289 0.21 -1.4 3,433,511 - rsmB b3289 0.22 -1.3 3,433,518 - rsmB b3289 0.22 -0.9 3,433,523 - rsmB b3289 0.23 +0.0 3,433,523 - rsmB b3289 0.23 -0.6 3,433,525 + rsmB b3289 0.23 +0.0 3,433,525 + rsmB b3289 0.23 -1.4 3,433,567 + rsmB b3289 0.26 -0.9 3,433,589 + rsmB b3289 0.28 -0.2 3,433,629 - rsmB b3289 0.31 -1.3 3,433,665 + rsmB b3289 0.34 -0.3 3,433,665 + rsmB b3289 0.34 +1.3 3,433,665 + rsmB b3289 0.34 -0.5 3,433,671 - rsmB b3289 0.34 -1.0 3,433,671 - rsmB b3289 0.34 -0.3 3,433,673 - rsmB b3289 0.34 -0.4 3,433,698 + rsmB b3289 0.36 +0.1 3,433,706 - rsmB b3289 0.37 -1.3 3,433,706 - rsmB b3289 0.37 -0.3 3,433,706 - rsmB b3289 0.37 -0.6 3,433,758 + rsmB b3289 0.41 -0.5 3,433,837 + rsmB b3289 0.47 -0.5 3,433,843 + rsmB b3289 0.48 +0.4 3,433,843 + rsmB b3289 0.48 -0.5 3,433,845 - rsmB b3289 0.48 -0.9 3,433,845 - rsmB b3289 0.48 +0.0 3,433,851 + rsmB b3289 0.48 +0.2 3,433,859 - rsmB b3289 0.49 -0.0 3,433,864 - rsmB b3289 0.49 +0.0 3,433,864 - rsmB b3289 0.49 -0.2 3,433,864 - rsmB b3289 0.49 -1.6 3,433,877 + rsmB b3289 0.50 -0.0 3,433,936 + rsmB b3289 0.55 -0.8 3,433,939 - rsmB b3289 0.55 -0.7 3,433,944 - rsmB b3289 0.55 -0.7 3,433,953 - rsmB b3289 0.56 -0.6 3,434,019 + rsmB b3289 0.61 -0.7 3,434,027 - rsmB b3289 0.62 -0.3 3,434,036 + rsmB b3289 0.63 -2.0 3,434,071 - rsmB b3289 0.65 -2.1 3,434,071 - rsmB b3289 0.65 -2.1 3,434,112 - rsmB b3289 0.68 +0.1 3,434,134 - -1.0 3,434,141 - rsmB b3289 0.71 -0.8 3,434,141 - rsmB b3289 0.71 -0.6 3,434,147 - rsmB b3289 0.71 -1.6 3,434,147 - rsmB b3289 0.71 -0.9 3,434,150 + rsmB b3289 0.71 -0.2 3,434,158 - rsmB b3289 0.72 -0.7 3,434,333 + rsmB b3289 0.86 +0.4 3,434,339 - rsmB b3289 0.86 -0.4 3,434,350 - rsmB b3289 0.87 -0.7 3,434,350 - rsmB b3289 0.87 -0.7 3,434,352 + rsmB b3289 0.87 +0.5 3,434,357 + rsmB b3289 0.87 -0.6 3,434,366 + rsmB b3289 0.88 -1.0 3,434,370 + rsmB b3289 0.88 -0.4 3,434,402 + -0.6 3,434,430 + -0.8 3,434,430 + +0.4 3,434,432 + -0.5 3,434,432 + -0.2 3,434,433 + -0.6 3,434,433 + -0.9 3,434,471 + -1.0 3,434,477 - -0.8 3,434,477 - +0.2 3,434,504 - +0.6 3,434,504 - -1.3 3,434,673 + +0.0 3,434,812 - trkA b3290 0.20 +1.0 3,434,850 + trkA b3290 0.23 +1.0 3,434,913 - trkA b3290 0.27 +1.0 3,434,918 - trkA b3290 0.27 +1.0 3,434,955 - trkA b3290 0.30 +0.0 3,434,955 - trkA b3290 0.30 +1.0 3,435,049 + trkA b3290 0.37 +1.0 3,435,185 + trkA b3290 0.47 +0.0 3,435,275 + trkA b3290 0.53 -1.0 3,435,314 - trkA b3290 0.56 +0.0 3,435,416 + trkA b3290 0.64 -1.0 3,435,474 - trkA b3290 0.68 +1.0 3,435,476 - trkA b3290 0.68 +1.0
Or see this region's nucleotide sequence