Experiment: BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcvP and gcvH are separated by 118 nucleotides gcvH and gcvT are separated by 23 nucleotides gcvT and visC are separated by 447 nucleotides
b2903: gcvP - glycine dehydrogenase (NCBI), at 3,044,190 to 3,047,063
gcvP
b2904: gcvH - glycine cleavage system protein H (NCBI), at 3,047,182 to 3,047,571
gcvH
b2905: gcvT - glycine cleavage system aminomethyltransferase T (NCBI), at 3,047,595 to 3,048,689
gcvT
b2906: visC - hypothetical protein (NCBI), at 3,049,137 to 3,050,339
visC
Position (kb)
3047
3048
3049 Strain fitness (log2 ratio)
-2
-1
0
1 at 3046.636 kb on + strand, within gcvP at 3046.636 kb on + strand, within gcvP at 3046.653 kb on - strand, within gcvP at 3046.659 kb on - strand, within gcvP at 3046.685 kb on - strand, within gcvP at 3046.693 kb on + strand, within gcvP at 3046.693 kb on + strand, within gcvP at 3046.693 kb on - strand, within gcvP at 3046.696 kb on - strand, within gcvP at 3046.706 kb on - strand, within gcvP at 3046.724 kb on + strand, within gcvP at 3046.724 kb on + strand, within gcvP at 3046.733 kb on + strand, within gcvP at 3046.803 kb on - strand at 3046.848 kb on + strand at 3046.854 kb on - strand at 3046.877 kb on - strand at 3047.124 kb on - strand at 3047.124 kb on - strand at 3047.214 kb on - strand at 3047.214 kb on - strand at 3047.321 kb on + strand, within gcvH at 3047.321 kb on + strand, within gcvH at 3047.468 kb on + strand, within gcvH at 3047.542 kb on + strand at 3047.542 kb on + strand at 3047.684 kb on + strand at 3047.765 kb on + strand, within gcvT at 3047.796 kb on + strand, within gcvT at 3047.865 kb on - strand, within gcvT at 3047.865 kb on - strand, within gcvT at 3047.871 kb on - strand, within gcvT at 3047.871 kb on - strand, within gcvT at 3048.013 kb on + strand, within gcvT at 3048.057 kb on - strand, within gcvT at 3048.128 kb on - strand, within gcvT at 3048.181 kb on - strand, within gcvT at 3048.181 kb on - strand, within gcvT at 3048.196 kb on - strand, within gcvT at 3048.196 kb on - strand, within gcvT at 3048.231 kb on - strand, within gcvT at 3048.231 kb on - strand, within gcvT at 3048.264 kb on - strand, within gcvT at 3048.306 kb on + strand, within gcvT at 3048.324 kb on - strand, within gcvT at 3048.339 kb on + strand, within gcvT at 3048.339 kb on + strand, within gcvT at 3048.399 kb on + strand, within gcvT at 3048.569 kb on + strand, within gcvT at 3048.569 kb on + strand, within gcvT at 3048.608 kb on - strand at 3048.624 kb on - strand at 3048.683 kb on + strand at 3048.683 kb on + strand at 3048.756 kb on + strand at 3048.756 kb on + strand at 3048.859 kb on - strand at 3048.859 kb on - strand at 3048.928 kb on - strand at 3049.195 kb on - strand at 3049.205 kb on - strand at 3049.205 kb on - strand at 3049.208 kb on - strand at 3049.208 kb on - strand at 3049.213 kb on - strand at 3049.310 kb on - strand, within visC at 3049.310 kb on - strand, within visC at 3049.315 kb on - strand, within visC at 3049.365 kb on + strand, within visC at 3049.406 kb on + strand, within visC at 3049.458 kb on + strand, within visC at 3049.474 kb on - strand, within visC at 3049.536 kb on + strand, within visC at 3049.544 kb on - strand, within visC at 3049.544 kb on - strand, within visC at 3049.546 kb on - strand, within visC at 3049.546 kb on - strand, within visC at 3049.546 kb on - strand, within visC at 3049.549 kb on + strand, within visC at 3049.563 kb on - strand, within visC at 3049.632 kb on + strand, within visC
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr remove 3,046,636 + gcvP b2903 0.85 -0.3 3,046,636 + gcvP b2903 0.85 -0.1 3,046,653 - gcvP b2903 0.86 -0.4 3,046,659 - gcvP b2903 0.86 +0.9 3,046,685 - gcvP b2903 0.87 -0.1 3,046,693 + gcvP b2903 0.87 +0.1 3,046,693 + gcvP b2903 0.87 +0.0 3,046,693 - gcvP b2903 0.87 -0.5 3,046,696 - gcvP b2903 0.87 -0.3 3,046,706 - gcvP b2903 0.88 -0.4 3,046,724 + gcvP b2903 0.88 -0.4 3,046,724 + gcvP b2903 0.88 -0.3 3,046,733 + gcvP b2903 0.88 -0.6 3,046,803 - +0.2 3,046,848 + -0.6 3,046,854 - +0.2 3,046,877 - -0.4 3,047,124 - +0.1 3,047,124 - +0.9 3,047,214 - -0.6 3,047,214 - -0.6 3,047,321 + gcvH b2904 0.36 -0.3 3,047,321 + gcvH b2904 0.36 -0.5 3,047,468 + gcvH b2904 0.73 -1.1 3,047,542 + -1.2 3,047,542 + -2.0 3,047,684 + -1.5 3,047,765 + gcvT b2905 0.16 -1.0 3,047,796 + gcvT b2905 0.18 +0.2 3,047,865 - gcvT b2905 0.25 -0.5 3,047,865 - gcvT b2905 0.25 -1.0 3,047,871 - gcvT b2905 0.25 -0.7 3,047,871 - gcvT b2905 0.25 -0.8 3,048,013 + gcvT b2905 0.38 -1.3 3,048,057 - gcvT b2905 0.42 -1.1 3,048,128 - gcvT b2905 0.49 +0.4 3,048,181 - gcvT b2905 0.54 -1.6 3,048,181 - gcvT b2905 0.54 -0.2 3,048,196 - gcvT b2905 0.55 -0.1 3,048,196 - gcvT b2905 0.55 +0.2 3,048,231 - gcvT b2905 0.58 +0.2 3,048,231 - gcvT b2905 0.58 -0.6 3,048,264 - gcvT b2905 0.61 -0.7 3,048,306 + gcvT b2905 0.65 -0.6 3,048,324 - gcvT b2905 0.67 -0.5 3,048,339 + gcvT b2905 0.68 -1.3 3,048,339 + gcvT b2905 0.68 -1.9 3,048,399 + gcvT b2905 0.73 -0.4 3,048,569 + gcvT b2905 0.89 -1.2 3,048,569 + gcvT b2905 0.89 -0.6 3,048,608 - -0.0 3,048,624 - -0.1 3,048,683 + -1.0 3,048,683 + -1.0 3,048,756 + -2.4 3,048,756 + -1.3 3,048,859 - +0.0 3,048,859 - -0.4 3,048,928 - -1.0 3,049,195 - -1.3 3,049,205 - -0.8 3,049,205 - -0.6 3,049,208 - -0.3 3,049,208 - +0.3 3,049,213 - -1.0 3,049,310 - visC b2906 0.14 -1.7 3,049,310 - visC b2906 0.14 -0.4 3,049,315 - visC b2906 0.15 -1.0 3,049,365 + visC b2906 0.19 -1.4 3,049,406 + visC b2906 0.22 -1.1 3,049,458 + visC b2906 0.27 -1.2 3,049,474 - visC b2906 0.28 -0.0 3,049,536 + visC b2906 0.33 -1.2 3,049,544 - visC b2906 0.34 -1.0 3,049,544 - visC b2906 0.34 -0.7 3,049,546 - visC b2906 0.34 -2.5 3,049,546 - visC b2906 0.34 -1.0 3,049,546 - visC b2906 0.34 +0.3 3,049,549 + visC b2906 0.34 -0.2 3,049,563 - visC b2906 0.35 -0.7 3,049,632 + visC b2906 0.41 -1.9
Or see this region's nucleotide sequence