Experiment: BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaA and moaB are separated by 21 nucleotides moaB and moaC are separated by 2 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 1 nucleotides moaE and ybhL are separated by 136 nucleotides
b0781: moaA - molybdenum cofactor biosynthesis protein A (NCBI), at 816,267 to 817,256
moaA
b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790
moaB
b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278
moaC
b0784: moaD - molybdopterin synthase, small subunit (NCBI), at 818,271 to 818,516
moaD
b0785: moaE - molybdopterin synthase, large subunit (NCBI), at 818,518 to 818,970
moaE
b0786: ybhL - predicted inner membrane protein (NCBI), at 819,107 to 819,811
ybhL
Position (kb) 817
818
819 Strain fitness (log2 ratio) -1
0
1 at 816.817 kb on + strand, within moaA at 816.912 kb on + strand, within moaA at 817.009 kb on - strand, within moaA at 817.009 kb on - strand, within moaA at 817.049 kb on - strand, within moaA at 817.085 kb on - strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.280 kb on - strand at 817.280 kb on - strand at 817.355 kb on - strand, within moaB at 817.485 kb on + strand, within moaB at 817.485 kb on + strand, within moaB at 817.818 kb on - strand at 817.818 kb on - strand at 817.852 kb on - strand, within moaC at 817.852 kb on - strand, within moaC at 817.872 kb on - strand, within moaC at 817.912 kb on + strand, within moaC at 817.912 kb on + strand, within moaC at 818.006 kb on - strand, within moaC at 818.012 kb on - strand, within moaC at 818.167 kb on - strand, within moaC at 818.211 kb on + strand, within moaC at 818.219 kb on - strand, within moaC at 818.219 kb on - strand, within moaC at 818.252 kb on + strand at 818.252 kb on + strand at 818.369 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.677 kb on - strand, within moaE at 818.687 kb on - strand, within moaE at 818.764 kb on - strand, within moaE at 818.892 kb on - strand, within moaE at 818.926 kb on + strand at 818.940 kb on - strand at 819.022 kb on + strand at 819.025 kb on - strand at 819.154 kb on + strand at 819.210 kb on + strand, within ybhL at 819.259 kb on + strand, within ybhL
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr remove 816,817 + moaA b0781 0.56 -0.8 816,912 + moaA b0781 0.65 -0.5 817,009 - moaA b0781 0.75 -0.3 817,009 - moaA b0781 0.75 -0.5 817,049 - moaA b0781 0.79 -0.6 817,085 - moaA b0781 0.83 -0.3 817,105 + moaA b0781 0.85 -0.2 817,105 + moaA b0781 0.85 -0.3 817,105 + moaA b0781 0.85 -0.4 817,280 - +0.5 817,280 - +0.5 817,355 - moaB b0782 0.15 -0.1 817,485 + moaB b0782 0.40 +0.9 817,485 + moaB b0782 0.40 +0.0 817,818 - -1.0 817,818 - -0.6 817,852 - moaC b0783 0.12 -1.4 817,852 - moaC b0783 0.12 -0.4 817,872 - moaC b0783 0.16 -1.6 817,912 + moaC b0783 0.24 -0.5 817,912 + moaC b0783 0.24 -1.7 818,006 - moaC b0783 0.44 -0.0 818,012 - moaC b0783 0.45 -1.6 818,167 - moaC b0783 0.77 -0.7 818,211 + moaC b0783 0.86 -0.6 818,219 - moaC b0783 0.88 -0.5 818,219 - moaC b0783 0.88 -0.5 818,252 + -0.4 818,252 + -0.8 818,369 + moaD b0784 0.40 -0.4 818,455 + moaD b0784 0.75 -0.1 818,455 + moaD b0784 0.75 +0.0 818,566 - moaE b0785 0.11 +0.2 818,566 - moaE b0785 0.11 -0.1 818,566 - moaE b0785 0.11 -0.0 818,677 - moaE b0785 0.35 -0.4 818,687 - moaE b0785 0.37 -1.0 818,764 - moaE b0785 0.54 -0.7 818,892 - moaE b0785 0.83 -0.3 818,926 + -0.4 818,940 - -1.1 819,022 + +0.3 819,025 - +0.3 819,154 + -0.3 819,210 + ybhL b0786 0.15 +0.5 819,259 + ybhL b0786 0.22 -0.1
Or see this region's nucleotide sequence