Experiment: BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kefC and folA are separated by 191 nucleotides folA and apaH are separated by 77 nucleotides apaH and apaG are separated by 6 nucleotides apaG and ksgA are separated by 2 nucleotides
b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631
kefC
b0048: folA - dihydrofolate reductase (NCBI), at 49,823 to 50,302
folA
b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222
apaH
b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606
apaG
b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430
ksgA
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 49.409 kb on - strand, within kefC at 49.431 kb on + strand, within kefC at 49.487 kb on - strand at 49.493 kb on - strand at 49.709 kb on + strand at 50.361 kb on + strand at 50.361 kb on + strand at 50.370 kb on - strand at 50.376 kb on + strand at 50.461 kb on + strand at 50.469 kb on - strand, within apaH at 50.501 kb on + strand, within apaH at 50.507 kb on - strand, within apaH at 50.568 kb on - strand, within apaH at 50.717 kb on - strand, within apaH at 50.785 kb on - strand, within apaH at 50.791 kb on + strand, within apaH at 50.802 kb on + strand, within apaH at 50.829 kb on - strand, within apaH at 50.859 kb on - strand, within apaH at 50.877 kb on + strand, within apaH at 50.877 kb on + strand, within apaH at 51.025 kb on + strand, within apaH at 51.109 kb on - strand, within apaH at 51.177 kb on + strand at 51.177 kb on + strand at 51.185 kb on - strand at 51.190 kb on + strand at 51.190 kb on + strand at 51.240 kb on + strand at 51.240 kb on + strand at 51.311 kb on + strand, within apaG at 51.311 kb on + strand, within apaG at 51.322 kb on - strand, within apaG at 51.326 kb on - strand, within apaG at 51.334 kb on - strand, within apaG at 51.336 kb on - strand, within apaG at 51.353 kb on + strand, within apaG at 51.353 kb on + strand, within apaG at 51.353 kb on - strand, within apaG at 51.364 kb on - strand, within apaG at 51.372 kb on - strand, within apaG at 51.415 kb on + strand, within apaG at 51.423 kb on - strand, within apaG at 51.466 kb on + strand, within apaG at 51.472 kb on + strand, within apaG at 51.512 kb on - strand, within apaG at 51.514 kb on - strand, within apaG at 51.520 kb on - strand, within apaG at 51.524 kb on + strand, within apaG at 51.550 kb on - strand, within apaG at 51.560 kb on + strand, within apaG at 51.567 kb on - strand, within apaG at 51.567 kb on - strand, within apaG at 51.576 kb on + strand at 51.576 kb on + strand at 51.586 kb on - strand at 51.613 kb on + strand at 51.614 kb on - strand at 51.615 kb on + strand at 51.615 kb on + strand at 51.673 kb on - strand at 51.703 kb on + strand, within ksgA at 51.703 kb on + strand, within ksgA at 51.812 kb on - strand, within ksgA at 51.836 kb on + strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.904 kb on + strand, within ksgA at 51.906 kb on + strand, within ksgA at 51.925 kb on - strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.045 kb on + strand, within ksgA at 52.062 kb on + strand, within ksgA at 52.062 kb on + strand at 52.070 kb on - strand, within ksgA at 52.072 kb on + strand, within ksgA at 52.089 kb on - strand, within ksgA
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS no stress control; Dimethyl Sulfoxide 1 vol% 24 hr remove 49,409 - kefC b0047 0.88 -0.1 49,431 + kefC b0047 0.89 -0.1 49,487 - +0.4 49,493 - +0.1 49,709 + -0.2 50,361 + +0.0 50,361 + +0.7 50,370 - -0.4 50,376 + -0.2 50,461 + +1.0 50,469 - apaH b0049 0.11 +1.0 50,501 + apaH b0049 0.14 +1.0 50,507 - apaH b0049 0.15 -0.6 50,568 - apaH b0049 0.22 +0.0 50,717 - apaH b0049 0.40 -1.8 50,785 - apaH b0049 0.48 -0.6 50,791 + apaH b0049 0.49 +1.0 50,802 + apaH b0049 0.50 +0.0 50,829 - apaH b0049 0.53 +1.0 50,859 - apaH b0049 0.57 -1.0 50,877 + apaH b0049 0.59 +1.0 50,877 + apaH b0049 0.59 +1.0 51,025 + apaH b0049 0.77 -0.6 51,109 - apaH b0049 0.86 -1.6 51,177 + +0.0 51,177 + +0.0 51,185 - +1.0 51,190 + +0.0 51,190 + -1.3 51,240 + +0.1 51,240 + -0.5 51,311 + apaG b0050 0.22 +0.2 51,311 + apaG b0050 0.22 +1.0 51,322 - apaG b0050 0.25 +0.6 51,326 - apaG b0050 0.26 +0.5 51,334 - apaG b0050 0.28 +0.1 51,336 - apaG b0050 0.28 +1.1 51,353 + apaG b0050 0.33 -1.3 51,353 + apaG b0050 0.33 -0.4 51,353 - apaG b0050 0.33 +0.0 51,364 - apaG b0050 0.36 -0.1 51,372 - apaG b0050 0.38 +0.6 51,415 + apaG b0050 0.49 -0.5 51,423 - apaG b0050 0.51 +0.3 51,466 + apaG b0050 0.63 -0.8 51,472 + apaG b0050 0.64 -0.3 51,512 - apaG b0050 0.75 +0.1 51,514 - apaG b0050 0.75 +0.9 51,520 - apaG b0050 0.77 +0.1 51,524 + apaG b0050 0.78 +0.3 51,550 - apaG b0050 0.85 -0.5 51,560 + apaG b0050 0.88 -1.1 51,567 - apaG b0050 0.89 +0.3 51,567 - apaG b0050 0.89 +0.2 51,576 + -0.7 51,576 + -2.6 51,586 - +0.2 51,613 + -0.3 51,614 - -0.3 51,615 + +0.6 51,615 + -0.7 51,673 - -0.3 51,703 + ksgA b0051 0.11 -0.2 51,703 + ksgA b0051 0.11 -2.2 51,812 - ksgA b0051 0.25 -2.6 51,836 + ksgA b0051 0.28 -2.2 51,891 - ksgA b0051 0.34 -0.6 51,891 - ksgA b0051 0.34 -0.3 51,904 + ksgA b0051 0.36 +1.0 51,906 + ksgA b0051 0.36 -1.6 51,925 - ksgA b0051 0.38 +1.6 52,040 + ksgA b0051 0.52 -1.3 52,040 + ksgA b0051 0.52 -0.6 52,040 + ksgA b0051 0.52 -0.3 52,042 + ksgA b0051 0.53 -3.9 52,042 + ksgA b0051 0.53 -1.6 52,042 + ksgA b0051 0.53 -1.8 52,042 - ksgA b0051 0.53 -2.0 52,042 - ksgA b0051 0.53 +0.0 52,045 + ksgA b0051 0.53 -1.0 52,062 + ksgA b0051 0.55 -1.2 52,062 + -0.6 52,070 - ksgA b0051 0.56 -2.0 52,072 + ksgA b0051 0.56 +0.0 52,089 - ksgA b0051 0.58 +0.4
Or see this region's nucleotide sequence