Experiment: M9 no stress control; Anaerobic 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycC and hycB overlap by 4 nucleotides hycB and hycA are separated by 124 nucleotides hycA and hypA are separated by 211 nucleotides hypA and hypB are separated by 3 nucleotides
b2723: hycC - NADH dehydrogenase subunit N (NCBI), at 2,845,437 to 2,847,263
hycC
b2724: hycB - hydrogenase 3, Fe-S subunit (NCBI), at 2,847,260 to 2,847,871
hycB
b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457
hycA
b2726: hypA - protein involved in nickel insertion into hydrogenases 3 (NCBI), at 2,848,669 to 2,849,019
hypA
b2727: hypB - GTP hydrolase involved in nickel liganding into hydrogenases (NCBI), at 2,849,023 to 2,849,895
hypB
Position (kb)
2847
2848
2849 Strain fitness (log2 ratio)
-2
-1
0
1 at 2847.072 kb on - strand, within hycC at 2847.099 kb on + strand at 2847.139 kb on - strand at 2847.276 kb on - strand at 2847.353 kb on + strand, within hycB at 2847.363 kb on + strand, within hycB at 2847.421 kb on + strand, within hycB at 2847.437 kb on - strand, within hycB at 2847.455 kb on + strand, within hycB at 2847.621 kb on + strand, within hycB at 2847.621 kb on + strand, within hycB at 2847.621 kb on - strand, within hycB at 2847.644 kb on + strand, within hycB at 2847.703 kb on + strand, within hycB at 2847.711 kb on - strand, within hycB at 2847.722 kb on - strand, within hycB at 2847.761 kb on - strand, within hycB at 2847.822 kb on - strand at 2847.829 kb on + strand at 2847.829 kb on + strand at 2847.888 kb on - strand at 2847.953 kb on - strand at 2848.071 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.082 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.684 kb on - strand at 2848.684 kb on - strand at 2848.684 kb on - strand at 2848.719 kb on - strand, within hypA at 2848.850 kb on - strand, within hypA at 2848.892 kb on + strand, within hypA at 2848.912 kb on + strand, within hypA at 2848.944 kb on + strand, within hypA at 2848.965 kb on - strand, within hypA at 2848.965 kb on - strand, within hypA at 2849.025 kb on + strand at 2849.025 kb on + strand at 2849.290 kb on - strand, within hypB at 2849.290 kb on - strand, within hypB at 2849.345 kb on + strand, within hypB at 2849.390 kb on - strand, within hypB at 2849.430 kb on + strand, within hypB at 2849.430 kb on + strand, within hypB
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Anaerobic 48 hr remove 2,847,072 - hycC b2723 0.89 +0.2 2,847,099 + +0.3 2,847,139 - +0.5 2,847,276 - +0.1 2,847,353 + hycB b2724 0.15 +0.3 2,847,363 + hycB b2724 0.17 -0.0 2,847,421 + hycB b2724 0.26 -0.5 2,847,437 - hycB b2724 0.29 -1.4 2,847,455 + hycB b2724 0.32 -0.2 2,847,621 + hycB b2724 0.59 -0.2 2,847,621 + hycB b2724 0.59 +0.2 2,847,621 - hycB b2724 0.59 +0.0 2,847,644 + hycB b2724 0.63 -0.1 2,847,703 + hycB b2724 0.72 +0.2 2,847,711 - hycB b2724 0.74 +0.2 2,847,722 - hycB b2724 0.75 -0.1 2,847,761 - hycB b2724 0.82 -0.5 2,847,822 - -0.4 2,847,829 + -0.0 2,847,829 + +0.4 2,847,888 - +0.2 2,847,953 - +0.3 2,848,071 - hycA b2725 0.16 -0.7 2,848,073 - hycA b2725 0.17 +0.6 2,848,073 - hycA b2725 0.17 -0.6 2,848,082 - hycA b2725 0.19 +0.4 2,848,153 - hycA b2725 0.34 -0.3 2,848,153 - hycA b2725 0.34 +1.2 2,848,157 + hycA b2725 0.35 +1.1 2,848,157 + hycA b2725 0.35 +0.7 2,848,253 - hycA b2725 0.56 +0.9 2,848,253 - hycA b2725 0.56 -0.1 2,848,684 - -0.7 2,848,684 - +0.4 2,848,684 - -0.2 2,848,719 - hypA b2726 0.14 +0.6 2,848,850 - hypA b2726 0.52 -0.3 2,848,892 + hypA b2726 0.64 -0.5 2,848,912 + hypA b2726 0.69 -0.9 2,848,944 + hypA b2726 0.78 +0.2 2,848,965 - hypA b2726 0.84 +0.3 2,848,965 - hypA b2726 0.84 +0.2 2,849,025 + +0.0 2,849,025 + +0.2 2,849,290 - hypB b2727 0.31 +0.2 2,849,290 - hypB b2727 0.31 -0.0 2,849,345 + hypB b2727 0.37 +0.2 2,849,390 - hypB b2727 0.42 -0.1 2,849,430 + hypB b2727 0.47 -2.4 2,849,430 + hypB b2727 0.47 -0.1
Or see this region's nucleotide sequence