Experiment: M9 no stress control; Anaerobic 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppk and ppx are separated by 4 nucleotides ppx and yfgF are separated by 38 nucleotides
b2501: ppk - polyphosphate kinase (NCBI), at 2,621,066 to 2,623,132
ppk
b2502: ppx - exopolyphosphatase (NCBI), at 2,623,137 to 2,624,678
ppx
b2503: yfgF - predicted inner membrane protein (NCBI), at 2,624,717 to 2,626,960
yfgF
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2622.137 kb on - strand, within ppk at 2622.151 kb on - strand, within ppk at 2622.151 kb on - strand, within ppk at 2622.248 kb on - strand, within ppk at 2622.268 kb on - strand, within ppk at 2622.324 kb on + strand, within ppk at 2622.329 kb on + strand, within ppk at 2622.345 kb on + strand, within ppk at 2622.388 kb on - strand, within ppk at 2622.461 kb on + strand, within ppk at 2622.461 kb on + strand, within ppk at 2622.471 kb on - strand, within ppk at 2622.550 kb on - strand, within ppk at 2622.628 kb on - strand, within ppk at 2622.647 kb on + strand, within ppk at 2622.647 kb on + strand, within ppk at 2622.667 kb on - strand, within ppk at 2622.667 kb on - strand, within ppk at 2622.702 kb on - strand, within ppk at 2622.702 kb on - strand, within ppk at 2622.720 kb on - strand, within ppk at 2622.729 kb on - strand, within ppk at 2622.782 kb on - strand, within ppk at 2622.839 kb on - strand, within ppk at 2622.897 kb on - strand, within ppk at 2622.923 kb on - strand, within ppk at 2623.010 kb on + strand at 2623.037 kb on - strand at 2623.112 kb on - strand at 2623.112 kb on - strand at 2623.135 kb on - strand at 2623.171 kb on + strand at 2623.220 kb on - strand at 2623.220 kb on - strand at 2623.304 kb on - strand, within ppx at 2623.395 kb on - strand, within ppx at 2623.398 kb on - strand, within ppx at 2623.429 kb on + strand, within ppx at 2623.532 kb on - strand, within ppx at 2623.677 kb on + strand, within ppx at 2623.677 kb on + strand, within ppx at 2623.720 kb on + strand, within ppx at 2623.720 kb on + strand, within ppx at 2623.755 kb on + strand, within ppx at 2623.755 kb on + strand, within ppx at 2623.756 kb on - strand, within ppx at 2623.769 kb on - strand, within ppx at 2623.769 kb on - strand, within ppx at 2623.941 kb on + strand, within ppx at 2623.941 kb on + strand, within ppx at 2623.942 kb on - strand, within ppx at 2623.996 kb on + strand, within ppx at 2624.019 kb on - strand, within ppx at 2624.040 kb on - strand at 2624.040 kb on - strand, within ppx at 2624.119 kb on - strand, within ppx at 2624.127 kb on + strand, within ppx at 2624.147 kb on + strand, within ppx at 2624.147 kb on + strand, within ppx at 2624.155 kb on - strand, within ppx at 2624.248 kb on + strand, within ppx at 2624.431 kb on + strand, within ppx at 2624.441 kb on + strand, within ppx at 2624.491 kb on + strand, within ppx at 2624.539 kb on - strand at 2624.539 kb on - strand at 2624.578 kb on - strand at 2624.579 kb on + strand at 2624.579 kb on + strand at 2624.601 kb on + strand at 2624.601 kb on + strand at 2624.797 kb on - strand at 2624.821 kb on - strand at 2624.821 kb on - strand at 2624.860 kb on - strand at 2625.005 kb on - strand, within yfgF at 2625.087 kb on - strand, within yfgF at 2625.092 kb on - strand, within yfgF at 2625.197 kb on + strand, within yfgF at 2625.206 kb on - strand, within yfgF at 2625.235 kb on + strand, within yfgF at 2625.412 kb on - strand, within yfgF at 2625.412 kb on - strand, within yfgF at 2625.545 kb on + strand, within yfgF at 2625.603 kb on - strand, within yfgF
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Anaerobic 48 hr remove 2,622,137 - ppk b2501 0.52 -3.2 2,622,151 - ppk b2501 0.52 -2.8 2,622,151 - ppk b2501 0.52 -0.2 2,622,248 - ppk b2501 0.57 -2.7 2,622,268 - ppk b2501 0.58 -3.4 2,622,324 + ppk b2501 0.61 -4.7 2,622,329 + ppk b2501 0.61 -2.9 2,622,345 + ppk b2501 0.62 -3.2 2,622,388 - ppk b2501 0.64 -0.8 2,622,461 + ppk b2501 0.67 -2.2 2,622,461 + ppk b2501 0.67 -1.4 2,622,471 - ppk b2501 0.68 -3.1 2,622,550 - ppk b2501 0.72 -3.5 2,622,628 - ppk b2501 0.76 -4.0 2,622,647 + ppk b2501 0.76 -0.4 2,622,647 + ppk b2501 0.76 -2.3 2,622,667 - ppk b2501 0.77 -2.8 2,622,667 - ppk b2501 0.77 -2.2 2,622,702 - ppk b2501 0.79 -2.5 2,622,702 - ppk b2501 0.79 -2.8 2,622,720 - ppk b2501 0.80 -0.4 2,622,729 - ppk b2501 0.80 -3.0 2,622,782 - ppk b2501 0.83 -3.5 2,622,839 - ppk b2501 0.86 -2.6 2,622,897 - ppk b2501 0.89 -3.1 2,622,923 - ppk b2501 0.90 -2.9 2,623,010 + -3.7 2,623,037 - -2.2 2,623,112 - +0.3 2,623,112 - -0.2 2,623,135 - -0.4 2,623,171 + +0.6 2,623,220 - +0.7 2,623,220 - +0.9 2,623,304 - ppx b2502 0.11 +1.0 2,623,395 - ppx b2502 0.17 +2.1 2,623,398 - ppx b2502 0.17 +0.3 2,623,429 + ppx b2502 0.19 +0.5 2,623,532 - ppx b2502 0.26 +0.4 2,623,677 + ppx b2502 0.35 -0.6 2,623,677 + ppx b2502 0.35 +0.1 2,623,720 + ppx b2502 0.38 +0.3 2,623,720 + ppx b2502 0.38 +0.5 2,623,755 + ppx b2502 0.40 +0.1 2,623,755 + ppx b2502 0.40 +0.4 2,623,756 - ppx b2502 0.40 +0.6 2,623,769 - ppx b2502 0.41 +0.5 2,623,769 - ppx b2502 0.41 +1.2 2,623,941 + ppx b2502 0.52 +0.1 2,623,941 + ppx b2502 0.52 +0.8 2,623,942 - ppx b2502 0.52 +0.9 2,623,996 + ppx b2502 0.56 +0.3 2,624,019 - ppx b2502 0.57 +0.6 2,624,040 - +0.5 2,624,040 - ppx b2502 0.59 +0.4 2,624,119 - ppx b2502 0.64 +0.6 2,624,127 + ppx b2502 0.64 +0.3 2,624,147 + ppx b2502 0.65 +1.0 2,624,147 + ppx b2502 0.65 +0.7 2,624,155 - ppx b2502 0.66 +0.7 2,624,248 + ppx b2502 0.72 +1.2 2,624,431 + ppx b2502 0.84 +0.1 2,624,441 + ppx b2502 0.85 +0.0 2,624,491 + ppx b2502 0.88 +0.8 2,624,539 - +0.9 2,624,539 - +0.1 2,624,578 - +0.9 2,624,579 + +0.8 2,624,579 + +0.6 2,624,601 + -0.1 2,624,601 + +0.1 2,624,797 - -0.2 2,624,821 - -0.4 2,624,821 - -0.2 2,624,860 - -0.0 2,625,005 - yfgF b2503 0.13 +0.3 2,625,087 - yfgF b2503 0.16 -0.0 2,625,092 - yfgF b2503 0.17 -0.1 2,625,197 + yfgF b2503 0.21 -0.2 2,625,206 - yfgF b2503 0.22 -1.3 2,625,235 + yfgF b2503 0.23 -0.2 2,625,412 - yfgF b2503 0.31 +0.2 2,625,412 - yfgF b2503 0.31 +0.3 2,625,545 + yfgF b2503 0.37 -0.4 2,625,603 - yfgF b2503 0.39 -0.1
Or see this region's nucleotide sequence