Experiment: M9 no stress control; Anaerobic 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2324 and yfcL are separated by 120 nucleotides yfcL and yfcM are separated by 33 nucleotides yfcM and yfcA overlap by 1 nucleotides yfcA and mepA overlap by 1 nucleotides
b2324: b2324 - putative peptidase (VIMSS), at 2,439,786 to 2,441,792
b2324
b2325: yfcL - hypothetical protein (NCBI), at 2,441,913 to 2,442,191
yfcL
b2326: yfcM - hypothetical protein (NCBI), at 2,442,225 to 2,442,773
yfcM
b2327: yfcA - conserved inner membrane protein (NCBI), at 2,442,773 to 2,443,582
yfcA
b2328: mepA - penicillin-insensitive murein endopeptidase (NCBI), at 2,443,582 to 2,444,406
mepA
Position (kb)
2442
2443 Strain fitness (log2 ratio)
-1
0
1
2 at 2441.329 kb on + strand, within b2324 at 2441.458 kb on + strand, within b2324 at 2441.471 kb on + strand, within b2324 at 2441.618 kb on - strand at 2441.625 kb on + strand at 2441.625 kb on + strand at 2441.751 kb on - strand at 2441.839 kb on + strand at 2441.883 kb on + strand at 2441.904 kb on - strand at 2441.966 kb on + strand, within yfcL at 2441.966 kb on + strand, within yfcL at 2442.015 kb on + strand, within yfcL at 2442.015 kb on + strand, within yfcL at 2442.033 kb on - strand, within yfcL at 2442.050 kb on + strand, within yfcL at 2442.050 kb on + strand, within yfcL at 2442.200 kb on - strand at 2442.221 kb on + strand at 2442.316 kb on - strand, within yfcM at 2442.343 kb on + strand, within yfcM at 2442.357 kb on - strand, within yfcM at 2442.440 kb on + strand, within yfcM at 2442.522 kb on + strand, within yfcM at 2442.583 kb on - strand, within yfcM at 2442.647 kb on - strand, within yfcM at 2442.647 kb on - strand, within yfcM at 2442.696 kb on - strand, within yfcM at 2442.758 kb on + strand at 2442.758 kb on + strand at 2442.758 kb on + strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.777 kb on - strand at 2442.807 kb on - strand at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.898 kb on + strand, within yfcA at 2442.906 kb on - strand, within yfcA at 2442.906 kb on - strand, within yfcA at 2442.948 kb on - strand, within yfcA at 2442.976 kb on + strand, within yfcA at 2442.985 kb on + strand, within yfcA at 2443.071 kb on - strand, within yfcA at 2443.161 kb on + strand, within yfcA at 2443.293 kb on - strand, within yfcA at 2443.330 kb on + strand, within yfcA at 2443.700 kb on - strand, within mepA at 2443.700 kb on - strand, within mepA at 2443.705 kb on + strand, within mepA at 2443.770 kb on + strand, within mepA
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Anaerobic 48 hr remove 2,441,329 + b2324 b2324 0.77 +0.9 2,441,458 + b2324 b2324 0.83 -0.4 2,441,471 + b2324 b2324 0.84 -0.2 2,441,618 - +0.6 2,441,625 + -0.6 2,441,625 + +0.1 2,441,751 - +0.2 2,441,839 + -0.6 2,441,883 + -0.6 2,441,904 - +0.4 2,441,966 + yfcL b2325 0.19 -0.2 2,441,966 + yfcL b2325 0.19 +0.7 2,442,015 + yfcL b2325 0.37 +0.3 2,442,015 + yfcL b2325 0.37 -1.2 2,442,033 - yfcL b2325 0.43 -0.7 2,442,050 + yfcL b2325 0.49 -0.6 2,442,050 + yfcL b2325 0.49 -1.5 2,442,200 - -1.1 2,442,221 + +0.3 2,442,316 - yfcM b2326 0.17 +0.4 2,442,343 + yfcM b2326 0.21 +1.8 2,442,357 - yfcM b2326 0.24 -0.0 2,442,440 + yfcM b2326 0.39 +0.4 2,442,522 + yfcM b2326 0.54 -0.2 2,442,583 - yfcM b2326 0.65 +0.0 2,442,647 - yfcM b2326 0.77 +0.1 2,442,647 - yfcM b2326 0.77 -0.2 2,442,696 - yfcM b2326 0.86 +0.6 2,442,758 + -0.0 2,442,758 + +0.7 2,442,758 + -0.7 2,442,766 - -0.3 2,442,766 - -0.6 2,442,766 - -0.6 2,442,766 - +0.0 2,442,777 - +0.1 2,442,807 - +0.3 2,442,887 + yfcA b2327 0.14 -0.7 2,442,887 + yfcA b2327 0.14 +0.9 2,442,887 + yfcA b2327 0.14 +0.2 2,442,887 + yfcA b2327 0.14 -0.2 2,442,898 + yfcA b2327 0.15 +0.4 2,442,906 - yfcA b2327 0.16 +0.0 2,442,906 - yfcA b2327 0.16 -0.7 2,442,948 - yfcA b2327 0.22 -1.2 2,442,976 + yfcA b2327 0.25 +0.1 2,442,985 + yfcA b2327 0.26 -0.8 2,443,071 - yfcA b2327 0.37 -1.1 2,443,161 + yfcA b2327 0.48 -0.1 2,443,293 - yfcA b2327 0.64 -0.1 2,443,330 + yfcA b2327 0.69 -0.5 2,443,700 - mepA b2328 0.14 -0.0 2,443,700 - mepA b2328 0.14 +0.2 2,443,705 + mepA b2328 0.15 -1.1 2,443,770 + mepA b2328 0.23 +0.4
Or see this region's nucleotide sequence