Experiment: M9 no stress control; Anaerobic 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gatD and gatC are separated by 47 nucleotides gatC and gatB are separated by 3 nucleotides gatB and gatA are separated by 30 nucleotides gatA and gatZ are separated by 9 nucleotides
b2091: gatD - galactitol-1-phosphate dehydrogenase, Zn-dependent and NAD(P)-binding (NCBI), at 2,169,857 to 2,170,897
gatD
b2092: gatC - galactitol-specific enzyme IIC component of PTS (NCBI), at 2,170,945 to 2,172,300
gatC
b2093: gatB - galactitol-specific enzyme IIB component of PTS (NCBI), at 2,172,304 to 2,172,588
gatB
b2094: gatA - galactitol-specific enzyme IIA component of PTS (NCBI), at 2,172,619 to 2,173,071
gatA
b2095: gatZ - D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI), at 2,173,081 to 2,174,343
gatZ
Position (kb)
2170
2171
2172
2173 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2169.996 kb on + strand, within gatD at 2170.033 kb on - strand, within gatD at 2170.231 kb on - strand, within gatD at 2170.231 kb on - strand, within gatD at 2170.265 kb on + strand, within gatD at 2170.272 kb on - strand, within gatD at 2170.273 kb on - strand, within gatD at 2170.273 kb on - strand, within gatD at 2170.342 kb on - strand, within gatD at 2170.342 kb on - strand, within gatD at 2170.361 kb on + strand, within gatD at 2170.361 kb on + strand, within gatD at 2170.364 kb on - strand, within gatD at 2170.379 kb on + strand, within gatD at 2170.379 kb on + strand, within gatD at 2170.379 kb on - strand, within gatD at 2170.379 kb on - strand, within gatD at 2170.432 kb on - strand, within gatD at 2170.452 kb on - strand, within gatD at 2170.624 kb on - strand, within gatD at 2170.624 kb on - strand, within gatD at 2170.665 kb on - strand, within gatD at 2170.668 kb on + strand, within gatD at 2170.676 kb on - strand, within gatD at 2170.676 kb on - strand at 2170.689 kb on + strand, within gatD at 2170.707 kb on - strand, within gatD at 2170.710 kb on - strand, within gatD at 2170.710 kb on - strand, within gatD at 2170.792 kb on - strand, within gatD at 2170.793 kb on - strand, within gatD at 2170.793 kb on - strand, within gatD at 2170.810 kb on + strand at 2170.810 kb on + strand at 2170.868 kb on - strand at 2170.873 kb on + strand at 2170.873 kb on - strand at 2170.912 kb on + strand at 2170.912 kb on + strand at 2171.087 kb on - strand, within gatC at 2171.226 kb on + strand, within gatC at 2171.404 kb on - strand, within gatC at 2171.562 kb on + strand, within gatC at 2171.566 kb on + strand, within gatC at 2171.583 kb on - strand, within gatC at 2171.732 kb on - strand, within gatC at 2171.762 kb on - strand, within gatC at 2171.762 kb on - strand, within gatC at 2171.771 kb on - strand, within gatC at 2171.771 kb on - strand, within gatC at 2171.871 kb on + strand, within gatC at 2171.871 kb on + strand, within gatC at 2171.879 kb on - strand, within gatC at 2171.879 kb on - strand, within gatC at 2171.960 kb on - strand, within gatC at 2171.960 kb on - strand, within gatC at 2172.073 kb on - strand, within gatC at 2172.130 kb on + strand, within gatC at 2172.137 kb on - strand, within gatC at 2172.247 kb on + strand at 2172.255 kb on + strand at 2172.285 kb on - strand at 2172.285 kb on - strand at 2172.295 kb on + strand at 2172.296 kb on - strand at 2172.303 kb on - strand at 2172.305 kb on - strand at 2172.315 kb on - strand at 2172.430 kb on - strand, within gatB at 2172.542 kb on - strand, within gatB at 2172.552 kb on - strand, within gatB at 2172.688 kb on - strand, within gatA at 2172.690 kb on - strand, within gatA at 2172.690 kb on - strand, within gatA at 2172.716 kb on - strand, within gatA at 2172.726 kb on - strand, within gatA at 2172.807 kb on - strand, within gatA at 2172.839 kb on - strand, within gatA at 2172.917 kb on - strand, within gatA at 2172.978 kb on - strand, within gatA at 2172.978 kb on - strand, within gatA at 2172.983 kb on + strand, within gatA at 2173.004 kb on - strand, within gatA at 2173.067 kb on + strand at 2173.075 kb on - strand at 2173.177 kb on - strand at 2173.177 kb on - strand at 2173.197 kb on - strand at 2173.197 kb on - strand at 2173.205 kb on - strand at 2173.205 kb on - strand at 2173.233 kb on - strand, within gatZ
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Anaerobic 48 hr remove 2,169,996 + gatD b2091 0.13 +0.4 2,170,033 - gatD b2091 0.17 -0.1 2,170,231 - gatD b2091 0.36 -0.2 2,170,231 - gatD b2091 0.36 +0.1 2,170,265 + gatD b2091 0.39 +0.3 2,170,272 - gatD b2091 0.40 -0.3 2,170,273 - gatD b2091 0.40 -0.4 2,170,273 - gatD b2091 0.40 +0.2 2,170,342 - gatD b2091 0.47 -1.0 2,170,342 - gatD b2091 0.47 -0.1 2,170,361 + gatD b2091 0.48 +0.8 2,170,361 + gatD b2091 0.48 +0.4 2,170,364 - gatD b2091 0.49 -0.1 2,170,379 + gatD b2091 0.50 -2.8 2,170,379 + gatD b2091 0.50 +2.7 2,170,379 - gatD b2091 0.50 +0.2 2,170,379 - gatD b2091 0.50 -0.3 2,170,432 - gatD b2091 0.55 -0.3 2,170,452 - gatD b2091 0.57 +0.1 2,170,624 - gatD b2091 0.74 -0.4 2,170,624 - gatD b2091 0.74 +0.5 2,170,665 - gatD b2091 0.78 +0.2 2,170,668 + gatD b2091 0.78 +0.2 2,170,676 - gatD b2091 0.79 +0.6 2,170,676 - -0.8 2,170,689 + gatD b2091 0.80 -1.2 2,170,707 - gatD b2091 0.82 -1.0 2,170,710 - gatD b2091 0.82 +0.1 2,170,710 - gatD b2091 0.82 -0.5 2,170,792 - gatD b2091 0.90 -0.4 2,170,793 - gatD b2091 0.90 -0.4 2,170,793 - gatD b2091 0.90 +0.2 2,170,810 + +1.1 2,170,810 + -0.2 2,170,868 - +0.5 2,170,873 + +0.0 2,170,873 - +0.2 2,170,912 + -0.9 2,170,912 + -1.7 2,171,087 - gatC b2092 0.10 +0.6 2,171,226 + gatC b2092 0.21 -0.9 2,171,404 - gatC b2092 0.34 +0.4 2,171,562 + gatC b2092 0.46 +0.7 2,171,566 + gatC b2092 0.46 +0.3 2,171,583 - gatC b2092 0.47 +1.1 2,171,732 - gatC b2092 0.58 +0.2 2,171,762 - gatC b2092 0.60 +0.4 2,171,762 - gatC b2092 0.60 +0.4 2,171,771 - gatC b2092 0.61 -0.5 2,171,771 - gatC b2092 0.61 +0.1 2,171,871 + gatC b2092 0.68 +2.1 2,171,871 + gatC b2092 0.68 -0.4 2,171,879 - gatC b2092 0.69 +0.8 2,171,879 - gatC b2092 0.69 +0.1 2,171,960 - gatC b2092 0.75 +0.1 2,171,960 - gatC b2092 0.75 +0.6 2,172,073 - gatC b2092 0.83 +0.3 2,172,130 + gatC b2092 0.87 +3.4 2,172,137 - gatC b2092 0.88 +0.4 2,172,247 + +0.9 2,172,255 + +0.6 2,172,285 - +0.4 2,172,285 - -0.0 2,172,295 + +0.5 2,172,296 - +0.5 2,172,303 - +0.3 2,172,305 - +0.4 2,172,315 - +0.4 2,172,430 - gatB b2093 0.44 +0.3 2,172,542 - gatB b2093 0.84 -0.0 2,172,552 - gatB b2093 0.87 +0.3 2,172,688 - gatA b2094 0.15 +0.1 2,172,690 - gatA b2094 0.16 +1.3 2,172,690 - gatA b2094 0.16 -0.2 2,172,716 - gatA b2094 0.21 +0.8 2,172,726 - gatA b2094 0.24 -0.7 2,172,807 - gatA b2094 0.42 +0.5 2,172,839 - gatA b2094 0.49 +0.1 2,172,917 - gatA b2094 0.66 -0.0 2,172,978 - gatA b2094 0.79 +0.1 2,172,978 - gatA b2094 0.79 +0.5 2,172,983 + gatA b2094 0.80 +1.1 2,173,004 - gatA b2094 0.85 -0.2 2,173,067 + -1.2 2,173,075 - +0.5 2,173,177 - +0.2 2,173,177 - +0.3 2,173,197 - -0.0 2,173,197 - +0.5 2,173,205 - -0.2 2,173,205 - -0.5 2,173,233 - gatZ b2095 0.12 +0.2
Or see this region's nucleotide sequence