Experiment: M9 no stress control; Anaerobic 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxH and ydhA are separated by 58 nucleotides ydhA and ydhH are separated by 97 nucleotides ydhH and slyB are separated by 273 nucleotides slyB and slyA are separated by 46 nucleotides
b1638: pdxH - pyridoxamine 5'-phosphate oxidase (NCBI), at 1,715,375 to 1,716,031
pdxH
b1639: ydhA - orf, hypothetical protein (VIMSS), at 1,716,090 to 1,716,419
ydhA
b1640: ydhH - anhydro-N-acetylmuramic acid kinase (NCBI), at 1,716,517 to 1,717,626
ydhH
b1641: slyB - outer membrane lipoprotein (NCBI), at 1,717,900 to 1,718,367
slyB
b1642: slyA - transcriptional regulator for cryptic hemolysin (VIMSS), at 1,718,414 to 1,718,848
slyA
Position (kb)
1716
1717
1718 Strain fitness (log2 ratio)
-1
0
1 at 1716.201 kb on - strand, within ydhA at 1716.224 kb on + strand, within ydhA at 1716.226 kb on + strand, within ydhA at 1716.226 kb on + strand, within ydhA at 1716.370 kb on - strand, within ydhA at 1716.375 kb on + strand, within ydhA at 1716.375 kb on + strand, within ydhA at 1716.447 kb on + strand at 1716.447 kb on + strand at 1716.447 kb on + strand at 1716.450 kb on - strand at 1716.554 kb on - strand at 1716.810 kb on + strand, within ydhH at 1716.989 kb on - strand, within ydhH at 1717.040 kb on + strand, within ydhH at 1717.040 kb on + strand, within ydhH at 1717.318 kb on + strand, within ydhH at 1717.569 kb on + strand at 1717.569 kb on + strand at 1717.612 kb on - strand at 1717.661 kb on + strand at 1717.661 kb on + strand at 1717.696 kb on - strand at 1717.837 kb on + strand at 1717.934 kb on + strand at 1718.034 kb on - strand, within slyB
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Anaerobic 48 hr remove 1,716,201 - ydhA b1639 0.34 -0.2 1,716,224 + ydhA b1639 0.41 -0.3 1,716,226 + ydhA b1639 0.41 -0.1 1,716,226 + ydhA b1639 0.41 +0.1 1,716,370 - ydhA b1639 0.85 -0.1 1,716,375 + ydhA b1639 0.86 -0.2 1,716,375 + ydhA b1639 0.86 +0.2 1,716,447 + -0.3 1,716,447 + +0.8 1,716,447 + +0.4 1,716,450 - -0.3 1,716,554 - -0.0 1,716,810 + ydhH b1640 0.26 +0.5 1,716,989 - ydhH b1640 0.43 +0.2 1,717,040 + ydhH b1640 0.47 +0.0 1,717,040 + ydhH b1640 0.47 +0.7 1,717,318 + ydhH b1640 0.72 +0.1 1,717,569 + -0.7 1,717,569 + +0.7 1,717,612 - +0.1 1,717,661 + +0.1 1,717,661 + -0.4 1,717,696 - -0.7 1,717,837 + +0.2 1,717,934 + -0.2 1,718,034 - slyB b1641 0.29 -0.2
Or see this region's nucleotide sequence