Experiment: M9 no stress control; Anaerobic 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oppF and b1248 are separated by 52 nucleotides b1248 and cls are separated by 34 nucleotides cls and yciY are separated by 142 nucleotides yciY and kch are separated by 54 nucleotides
b1247: oppF - oligopeptide transporter subunit (NCBI), at 1,303,788 to 1,304,792
oppF
b1248: b1248 - orf, hypothetical protein (VIMSS), at 1,304,845 to 1,305,174
b1248
b1249: cls - cardiolipin synthetase (NCBI), at 1,305,209 to 1,306,669
cls
b4595: yciY - hypothetical protein (RefSeq), at 1,306,812 to 1,306,985
yciY
b1250: kch - voltage-gated potassium channel (NCBI), at 1,307,040 to 1,308,293
kch
Position (kb)
1305
1306
1307 Strain fitness (log2 ratio)
-1
0
1
2 at 1304.294 kb on + strand, within oppF at 1304.294 kb on + strand, within oppF at 1304.367 kb on + strand, within oppF at 1304.720 kb on + strand at 1304.780 kb on - strand at 1304.780 kb on - strand at 1305.078 kb on - strand, within b1248 at 1305.078 kb on - strand, within b1248 at 1305.176 kb on + strand at 1305.186 kb on + strand at 1305.539 kb on + strand, within cls at 1305.539 kb on + strand, within cls at 1305.576 kb on - strand, within cls at 1305.588 kb on - strand, within cls at 1305.588 kb on - strand, within cls at 1305.625 kb on - strand, within cls at 1305.641 kb on + strand, within cls at 1305.641 kb on + strand, within cls at 1305.899 kb on + strand, within cls at 1305.959 kb on + strand, within cls at 1306.053 kb on - strand, within cls at 1306.071 kb on - strand, within cls at 1306.203 kb on + strand, within cls at 1306.258 kb on - strand, within cls at 1306.303 kb on + strand, within cls at 1306.303 kb on + strand, within cls at 1306.401 kb on + strand, within cls at 1306.401 kb on + strand, within cls at 1306.625 kb on - strand at 1306.762 kb on - strand at 1306.782 kb on - strand at 1306.955 kb on + strand, within yciY at 1306.955 kb on + strand, within yciY at 1306.998 kb on + strand at 1307.001 kb on + strand at 1307.013 kb on + strand at 1307.235 kb on - strand, within kch at 1307.325 kb on - strand, within kch at 1307.331 kb on + strand, within kch at 1307.342 kb on + strand, within kch at 1307.350 kb on - strand, within kch at 1307.350 kb on - strand, within kch at 1307.350 kb on - strand, within kch at 1307.353 kb on + strand, within kch at 1307.353 kb on + strand, within kch at 1307.353 kb on + strand, within kch at 1307.387 kb on - strand, within kch at 1307.431 kb on + strand, within kch at 1307.441 kb on + strand, within kch at 1307.444 kb on + strand, within kch at 1307.510 kb on + strand, within kch at 1307.524 kb on + strand, within kch at 1307.524 kb on + strand, within kch at 1307.613 kb on + strand, within kch at 1307.613 kb on + strand, within kch at 1307.620 kb on - strand, within kch
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Anaerobic 48 hr remove 1,304,294 + oppF b1247 0.50 +0.3 1,304,294 + oppF b1247 0.50 +0.2 1,304,367 + oppF b1247 0.58 +0.0 1,304,720 + +0.0 1,304,780 - +0.3 1,304,780 - +0.2 1,305,078 - b1248 b1248 0.71 -0.6 1,305,078 - b1248 b1248 0.71 -0.3 1,305,176 + +0.3 1,305,186 + +0.2 1,305,539 + cls b1249 0.23 -0.1 1,305,539 + cls b1249 0.23 +0.6 1,305,576 - cls b1249 0.25 +1.2 1,305,588 - cls b1249 0.26 +0.6 1,305,588 - cls b1249 0.26 -0.4 1,305,625 - cls b1249 0.28 +0.1 1,305,641 + cls b1249 0.30 +0.4 1,305,641 + cls b1249 0.30 -0.1 1,305,899 + cls b1249 0.47 +0.6 1,305,959 + cls b1249 0.51 +1.4 1,306,053 - cls b1249 0.58 +0.3 1,306,071 - cls b1249 0.59 +0.6 1,306,203 + cls b1249 0.68 +0.6 1,306,258 - cls b1249 0.72 +0.7 1,306,303 + cls b1249 0.75 +0.3 1,306,303 + cls b1249 0.75 -0.1 1,306,401 + cls b1249 0.82 +0.2 1,306,401 + cls b1249 0.82 -0.2 1,306,625 - +0.7 1,306,762 - +1.5 1,306,782 - +1.1 1,306,955 + yciY b4595 0.82 +0.2 1,306,955 + yciY b4595 0.82 +0.9 1,306,998 + +2.1 1,307,001 + +0.3 1,307,013 + -0.9 1,307,235 - kch b1250 0.16 +0.6 1,307,325 - kch b1250 0.23 +0.7 1,307,331 + kch b1250 0.23 +0.8 1,307,342 + kch b1250 0.24 +1.1 1,307,350 - kch b1250 0.25 -0.1 1,307,350 - kch b1250 0.25 +0.1 1,307,350 - kch b1250 0.25 -0.9 1,307,353 + kch b1250 0.25 +0.6 1,307,353 + kch b1250 0.25 -0.1 1,307,353 + kch b1250 0.25 -0.1 1,307,387 - kch b1250 0.28 -0.2 1,307,431 + kch b1250 0.31 +1.1 1,307,441 + kch b1250 0.32 -1.5 1,307,444 + kch b1250 0.32 +0.3 1,307,510 + kch b1250 0.37 +1.1 1,307,524 + kch b1250 0.39 +0.5 1,307,524 + kch b1250 0.39 -0.6 1,307,613 + kch b1250 0.46 +0.5 1,307,613 + kch b1250 0.46 -0.1 1,307,620 - kch b1250 0.46 +1.2
Or see this region's nucleotide sequence