Experiment: M9 no stress control; Anaerobic 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citC and citA are separated by 378 nucleotides citA and citB overlap by 32 nucleotides citB and dcuC are separated by 40 nucleotides
b0618: citC - citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS), at 650,021 to 651,079
citC
b0619: citA - sensory histidine kinase in two-component regulatory system with citB (NCBI), at 651,458 to 653,116
citA
b0620: citB - DNA-binding response regulator in two-component regulatory system with citA (NCBI), at 653,085 to 653,765
citB
b0621: dcuC - anaerobic C4-dicarboxylate transport (NCBI), at 653,806 to 655,191
dcuC
Position (kb) 651
652
653
654 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 650.577 kb on + strand, within citC at 650.733 kb on - strand, within citC at 650.794 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.816 kb on - strand, within citC at 650.818 kb on + strand, within citC at 650.818 kb on + strand, within citC at 650.818 kb on + strand, within citC at 650.858 kb on + strand, within citC at 650.860 kb on - strand, within citC at 650.863 kb on + strand, within citC at 650.863 kb on + strand, within citC at 650.863 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.888 kb on + strand, within citC at 650.888 kb on + strand, within citC at 650.888 kb on - strand, within citC at 650.955 kb on + strand, within citC at 650.955 kb on + strand, within citC at 650.962 kb on + strand, within citC at 650.966 kb on + strand, within citC at 650.966 kb on + strand, within citC at 650.978 kb on + strand at 650.978 kb on + strand at 650.988 kb on + strand at 650.988 kb on + strand at 650.992 kb on + strand at 650.992 kb on + strand at 651.085 kb on + strand at 651.218 kb on + strand at 651.525 kb on - strand at 651.551 kb on - strand at 651.606 kb on - strand at 651.606 kb on - strand at 651.611 kb on + strand at 651.616 kb on + strand at 651.622 kb on - strand at 651.622 kb on - strand at 651.629 kb on - strand, within citA at 651.629 kb on - strand, within citA at 651.657 kb on + strand, within citA at 651.661 kb on - strand, within citA at 651.663 kb on - strand, within citA at 651.669 kb on + strand, within citA at 651.671 kb on - strand, within citA at 651.671 kb on - strand, within citA at 651.677 kb on - strand, within citA at 651.690 kb on + strand, within citA at 651.711 kb on + strand, within citA at 651.711 kb on + strand, within citA at 651.784 kb on - strand, within citA at 651.810 kb on + strand, within citA at 651.810 kb on + strand, within citA at 651.818 kb on - strand, within citA at 651.818 kb on - strand, within citA at 651.876 kb on + strand, within citA at 651.877 kb on + strand, within citA at 652.096 kb on - strand, within citA at 652.232 kb on - strand, within citA at 652.321 kb on + strand, within citA at 652.344 kb on + strand, within citA at 652.344 kb on + strand, within citA at 652.393 kb on + strand, within citA at 652.522 kb on + strand, within citA at 652.522 kb on + strand, within citA at 652.799 kb on + strand, within citA at 652.799 kb on + strand, within citA at 652.880 kb on + strand, within citA at 652.885 kb on + strand, within citA at 652.893 kb on - strand, within citA at 652.966 kb on - strand at 652.968 kb on + strand at 652.981 kb on - strand at 653.038 kb on + strand at 653.039 kb on - strand at 653.081 kb on + strand at 653.081 kb on + strand at 653.081 kb on + strand at 653.094 kb on + strand at 653.094 kb on + strand at 653.094 kb on + strand at 653.094 kb on + strand at 653.183 kb on - strand, within citB at 653.183 kb on - strand, within citB at 653.213 kb on + strand, within citB at 653.213 kb on + strand, within citB at 653.236 kb on - strand, within citB at 653.238 kb on + strand, within citB at 653.239 kb on + strand, within citB at 653.285 kb on - strand, within citB at 653.328 kb on + strand, within citB at 653.330 kb on + strand, within citB at 653.336 kb on - strand, within citB at 653.350 kb on + strand, within citB at 653.378 kb on - strand, within citB at 653.378 kb on - strand, within citB at 653.381 kb on - strand, within citB at 653.385 kb on + strand, within citB at 653.510 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.542 kb on - strand, within citB at 653.542 kb on - strand, within citB at 653.557 kb on - strand, within citB at 653.635 kb on + strand, within citB at 653.635 kb on + strand, within citB at 653.646 kb on + strand, within citB at 653.646 kb on + strand, within citB at 653.647 kb on - strand, within citB at 653.659 kb on + strand, within citB at 653.659 kb on + strand, within citB at 653.747 kb on - strand at 653.747 kb on - strand at 653.759 kb on - strand at 653.759 kb on - strand at 653.831 kb on + strand at 653.858 kb on - strand at 653.886 kb on - strand at 653.886 kb on - strand at 653.967 kb on + strand, within dcuC
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Anaerobic 48 hr remove 650,577 + citC b0618 0.53 -0.3 650,733 - citC b0618 0.67 -0.4 650,794 + citC b0618 0.73 -0.2 650,808 + citC b0618 0.74 -0.3 650,808 + citC b0618 0.74 +0.1 650,808 + citC b0618 0.74 +0.6 650,816 - citC b0618 0.75 -0.0 650,818 + citC b0618 0.75 +0.9 650,818 + citC b0618 0.75 +0.0 650,818 + citC b0618 0.75 +2.0 650,858 + citC b0618 0.79 -0.1 650,860 - citC b0618 0.79 -2.1 650,863 + citC b0618 0.80 -0.9 650,863 + citC b0618 0.80 +0.1 650,863 + citC b0618 0.80 -0.3 650,885 + citC b0618 0.82 -0.8 650,885 + citC b0618 0.82 -1.1 650,885 + citC b0618 0.82 -1.0 650,888 + citC b0618 0.82 -0.3 650,888 + citC b0618 0.82 -0.0 650,888 - citC b0618 0.82 +1.2 650,955 + citC b0618 0.88 +0.7 650,955 + citC b0618 0.88 +1.2 650,962 + citC b0618 0.89 +2.2 650,966 + citC b0618 0.89 -0.2 650,966 + citC b0618 0.89 +0.0 650,978 + +0.8 650,978 + +1.2 650,988 + +3.0 650,988 + +0.4 650,992 + +0.8 650,992 + +1.2 651,085 + +1.2 651,218 + +0.2 651,525 - +1.8 651,551 - +1.2 651,606 - +1.2 651,606 - +1.2 651,611 + -1.1 651,616 + +2.8 651,622 - +1.4 651,622 - +0.2 651,629 - citA b0619 0.10 -0.2 651,629 - citA b0619 0.10 +0.8 651,657 + citA b0619 0.12 -1.1 651,661 - citA b0619 0.12 +0.9 651,663 - citA b0619 0.12 +0.7 651,669 + citA b0619 0.13 -0.9 651,671 - citA b0619 0.13 -0.4 651,671 - citA b0619 0.13 -2.5 651,677 - citA b0619 0.13 +1.8 651,690 + citA b0619 0.14 -0.3 651,711 + citA b0619 0.15 -1.0 651,711 + citA b0619 0.15 -0.7 651,784 - citA b0619 0.20 -0.2 651,810 + citA b0619 0.21 -2.2 651,810 + citA b0619 0.21 -1.1 651,818 - citA b0619 0.22 -0.6 651,818 - citA b0619 0.22 +0.2 651,876 + citA b0619 0.25 -1.5 651,877 + citA b0619 0.25 -1.6 652,096 - citA b0619 0.38 +0.1 652,232 - citA b0619 0.47 -0.3 652,321 + citA b0619 0.52 -2.2 652,344 + citA b0619 0.53 -2.2 652,344 + citA b0619 0.53 -1.1 652,393 + citA b0619 0.56 -2.7 652,522 + citA b0619 0.64 -4.3 652,522 + citA b0619 0.64 -2.3 652,799 + citA b0619 0.81 -3.6 652,799 + citA b0619 0.81 -1.9 652,880 + citA b0619 0.86 -2.4 652,885 + citA b0619 0.86 -2.4 652,893 - citA b0619 0.86 +0.1 652,966 - -0.1 652,968 + -2.5 652,981 - +0.1 653,038 + -3.0 653,039 - +1.0 653,081 + +0.1 653,081 + -2.6 653,081 + -2.7 653,094 + +0.2 653,094 + -0.8 653,094 + +0.4 653,094 + -0.4 653,183 - citB b0620 0.14 -0.0 653,183 - citB b0620 0.14 -0.3 653,213 + citB b0620 0.19 -0.1 653,213 + citB b0620 0.19 +0.0 653,236 - citB b0620 0.22 -0.8 653,238 + citB b0620 0.22 +0.1 653,239 + citB b0620 0.23 -0.1 653,285 - citB b0620 0.29 -0.4 653,328 + citB b0620 0.36 -0.0 653,330 + citB b0620 0.36 +0.1 653,336 - citB b0620 0.37 -0.1 653,350 + citB b0620 0.39 -0.6 653,378 - citB b0620 0.43 -1.2 653,378 - citB b0620 0.43 +0.3 653,381 - citB b0620 0.43 -0.5 653,385 + citB b0620 0.44 +0.1 653,510 + citB b0620 0.62 +0.4 653,534 + citB b0620 0.66 +0.2 653,534 + citB b0620 0.66 +0.5 653,534 + citB b0620 0.66 -0.3 653,534 + citB b0620 0.66 -0.4 653,534 + citB b0620 0.66 +0.1 653,542 - citB b0620 0.67 +0.0 653,542 - citB b0620 0.67 +0.1 653,557 - citB b0620 0.69 +0.4 653,635 + citB b0620 0.81 +0.1 653,635 + citB b0620 0.81 +0.1 653,646 + citB b0620 0.82 +0.4 653,646 + citB b0620 0.82 -0.5 653,647 - citB b0620 0.83 -1.3 653,659 + citB b0620 0.84 -1.8 653,659 + citB b0620 0.84 -0.2 653,747 - +0.2 653,747 - +1.3 653,759 - -0.0 653,759 - -0.1 653,831 + -0.0 653,858 - +0.2 653,886 - +0.2 653,886 - -0.7 653,967 + dcuC b0621 0.12 +0.4
Or see this region's nucleotide sequence