Experiment: M9 no stress control; Anaerobic 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yacG and yacF are separated by 9 nucleotides yacF and coaE overlap by 1 nucleotides coaE and guaC are separated by 224 nucleotides
b0101: yacG - zinc-binding protein (NCBI), at 111,649 to 111,846
yacG
b0102: yacF - hypothetical protein (NCBI), at 111,856 to 112,599
yacF
b0103: coaE - dephospho-CoA kinase (NCBI), at 112,599 to 113,219
coaE
b0104: guaC - guanosine 5'-monophosphate oxidoreductase (NCBI), at 113,444 to 114,487
guaC
Position (kb)
112
113
114 Strain fitness (log2 ratio)
-2
-1
0
1 at 111.616 kb on - strand at 111.792 kb on - strand, within yacG at 111.795 kb on + strand, within yacG at 111.795 kb on + strand, within yacG at 111.823 kb on + strand, within yacG at 111.828 kb on + strand at 111.828 kb on + strand at 111.836 kb on - strand at 111.836 kb on - strand at 111.886 kb on + strand at 111.995 kb on - strand, within yacF at 111.997 kb on + strand, within yacF at 112.005 kb on - strand at 112.005 kb on - strand, within yacF at 112.089 kb on - strand, within yacF at 112.089 kb on - strand, within yacF at 112.155 kb on + strand, within yacF at 112.188 kb on - strand, within yacF at 112.347 kb on + strand, within yacF at 112.347 kb on + strand, within yacF at 112.347 kb on + strand, within yacF at 112.366 kb on + strand, within yacF at 112.397 kb on - strand, within yacF at 112.553 kb on - strand at 112.553 kb on - strand at 113.326 kb on + strand at 113.357 kb on + strand at 113.367 kb on + strand at 113.375 kb on - strand at 113.375 kb on - strand at 113.379 kb on + strand at 113.379 kb on + strand at 113.384 kb on + strand at 113.384 kb on + strand at 113.400 kb on + strand at 113.446 kb on - strand at 113.475 kb on + strand at 113.480 kb on - strand at 113.503 kb on - strand at 113.510 kb on + strand at 113.510 kb on + strand at 113.564 kb on + strand, within guaC at 113.668 kb on + strand, within guaC at 113.668 kb on + strand, within guaC at 113.788 kb on + strand, within guaC at 113.788 kb on + strand, within guaC at 113.803 kb on + strand, within guaC at 113.803 kb on + strand, within guaC at 113.854 kb on + strand, within guaC at 114.020 kb on + strand, within guaC at 114.039 kb on + strand, within guaC at 114.075 kb on + strand, within guaC at 114.075 kb on + strand, within guaC at 114.082 kb on + strand, within guaC at 114.082 kb on + strand, within guaC at 114.121 kb on - strand, within guaC at 114.121 kb on - strand, within guaC at 114.191 kb on - strand, within guaC at 114.191 kb on - strand, within guaC at 114.217 kb on - strand, within guaC
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Anaerobic 48 hr remove 111,616 - -0.2 111,792 - yacG b0101 0.72 -0.3 111,795 + yacG b0101 0.74 -0.7 111,795 + yacG b0101 0.74 -0.3 111,823 + yacG b0101 0.88 -0.2 111,828 + -0.0 111,828 + -0.6 111,836 - +0.4 111,836 - +0.7 111,886 + -0.1 111,995 - yacF b0102 0.19 +0.1 111,997 + yacF b0102 0.19 -0.2 112,005 - -1.8 112,005 - yacF b0102 0.20 -0.1 112,089 - yacF b0102 0.31 -0.3 112,089 - yacF b0102 0.31 +0.3 112,155 + yacF b0102 0.40 +0.5 112,188 - yacF b0102 0.45 +0.1 112,347 + yacF b0102 0.66 +0.0 112,347 + yacF b0102 0.66 -0.5 112,347 + yacF b0102 0.66 -0.6 112,366 + yacF b0102 0.69 +0.7 112,397 - yacF b0102 0.73 -0.5 112,553 - -0.2 112,553 - +0.0 113,326 + -0.0 113,357 + +0.3 113,367 + +0.4 113,375 - -0.4 113,375 - +0.3 113,379 + -0.2 113,379 + -0.3 113,384 + -0.4 113,384 + -0.3 113,400 + -0.3 113,446 - +0.3 113,475 + +0.6 113,480 - +0.5 113,503 - +0.1 113,510 + +0.2 113,510 + +0.2 113,564 + guaC b0104 0.11 +0.1 113,668 + guaC b0104 0.21 +0.3 113,668 + guaC b0104 0.21 +0.5 113,788 + guaC b0104 0.33 +0.4 113,788 + guaC b0104 0.33 +0.0 113,803 + guaC b0104 0.34 -0.2 113,803 + guaC b0104 0.34 -0.4 113,854 + guaC b0104 0.39 +0.1 114,020 + guaC b0104 0.55 +0.5 114,039 + guaC b0104 0.57 +0.6 114,075 + guaC b0104 0.60 +0.4 114,075 + guaC b0104 0.60 -0.1 114,082 + guaC b0104 0.61 +0.7 114,082 + guaC b0104 0.61 +1.4 114,121 - guaC b0104 0.65 +0.1 114,121 - guaC b0104 0.65 +0.0 114,191 - guaC b0104 0.72 +0.4 114,191 - guaC b0104 0.72 +0.2 114,217 - guaC b0104 0.74 +0.7
Or see this region's nucleotide sequence